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Electric Field Modulation of Electron Transfer in Bacterial Photosynthetic Reaction Centers

  • Z. Popovic
  • G. Kovacs
  • P. Vincett
  • G. Alegria
  • P. L. Dutton
Part of the Proceedings in Life Sciences book series (LIFE SCIENCES)

Abstract

Multilayer Langmuir-Blodgett (LB) films of reaction centers from the photosynthetic bacterium Rhodopseudomonas sphaeroides have been fabricated with partial net orientation. From measurements of the light induced electron transfer reactions in reaction center films, we have succeeded in quantitating the electric field dependence of (1) the quantum yield of charge separation and (2) the kinetics of charge recombination.

Keywords

Quantum Yield Reaction Center Charge Separation Probe Beam Photosynthetic Bacterium 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Clayton, R.K. and Sistrom, W.R. (1978) “The Photosynthetic Bacteria”, Plenum Press, New York.Google Scholar
  2. 2.
    Kaufman, K.J., Dutton, P.L., Netzel, T.L. and Rentzepis, P.M. (1975) Science 188, 1301–1304.ADSCrossRefGoogle Scholar
  3. 3.
    Pockley, M.G., Windsor, M.W., Cogdell, R.J. and Parson, W.W. (1975) Proc. Natl. Acad. Sci. 72, 2251–2259.ADSCrossRefGoogle Scholar
  4. 4.
    Shuralow, V.A., Klenvanik, A.V., Sharkov, A.V., Matveetz, Y.A. and Krukov, P.G. (1978) FEBS Letts. 91, 135–139.CrossRefGoogle Scholar
  5. 5.
    Holten, D., Windsor, M.W., Parson, W.W. and Thornber, J.P. (1978) Biochim. Biophys. Acta 501, 112–126.CrossRefGoogle Scholar
  6. 6.
    Kaufman, K.J., Petty, K.M., Dutton, P.L. and Rentzepis, P.M. (1976) Biochim. Biophys. Res. Comm. 70, 839–845.CrossRefGoogle Scholar
  7. 7.
    Cogdell, R.J., Monger, T.G. and Parson, W.W. (1975) Biochim. Biophys. Acta 408, 189–199.CrossRefGoogle Scholar
  8. 8.
    Parson, W.W., Clayton, R.K. and Cogdell, R.J. (1975) Biochim. Biophys. Acta 387, 286–278.CrossRefGoogle Scholar
  9. 9.
    Parson, W.W. and Monger, T.G. (1977) Brookhaven Symp. Biol. 28, 195–211.Google Scholar
  10. 10.
    Holten, D., Windsor, M.W., Parson, W.W. and Thornber, J.P. (1978b) Biochim. Biophys. Acta 501, 112–126.CrossRefGoogle Scholar
  11. 11.
    Woodbury, N.T. and Parson, W.W. (1984) Biochim. Biophys. Acta 767, 345–361.CrossRefGoogle Scholar
  12. 12.
    Schenk, C.C., Blankenship, R.E. and Parson, W.W. (1982) Biochim. Biophys. Acta 680, 44–59.CrossRefGoogle Scholar
  13. 13.
    Prince, R.C. and Dutton, P.L. (1978) in “The Photosynthetic Bacteria” (Clayton, R.K. and Sistrom, W.R., eds.) pp. 440–453 Plenum Press, New York.Google Scholar
  14. 14.
    Prince, R.C. and Dutton, P.L. (1976) Arch. Biochem. Biophys. 172, 329–334.CrossRefGoogle Scholar
  15. 15.
    Dutton, P.L. Leigh, J.S. (1973) Biochim. Biophys. Acta 292, 654–664.CrossRefGoogle Scholar
  16. 16.
    Blasie, J.K., Pachene, J.M., Tavormina, A., Dutton, P.L., Stamatoff, J., Eisenberger, P. and Brown, G. (1983) Biochim. Biophys. Acta 723, 350–357.CrossRefGoogle Scholar
  17. 17.
    Govindjee, (1982) “Photosynthesis: Energy Conversion by Plants and Bacteria”, Academic Press New York.Google Scholar
  18. 18.
    Valkires, G.E. and Feher, G. (1976) J. Cell Biol. 95, 179–188.CrossRefGoogle Scholar
  19. 19.
    Pachence, J.M., Dutton, P.L. and Blasie, J.K. (1979) Biochim. Biophys. Acta 548, 348–373.CrossRefGoogle Scholar
  20. 20.
    Bachman, R.C., Gillies, K. and Takamoto, J.M. (1981) Biochemistry 20, 4590–4596.CrossRefGoogle Scholar
  21. 21.
    Jackson, J.B. and Crofts, A.R. (1969) FEBS Letts. 4, 185–189.CrossRefGoogle Scholar
  22. 22.
    Jackson, J.B. and Dutton, P.L. (1973) Biochim. Biophys. Acta 325, 102–113.CrossRefGoogle Scholar
  23. 23.
    Evans, E.H. and Crofts, A.R. (1974) Biochim. Biophys. Acta 333, 44–51.CrossRefGoogle Scholar
  24. 24.
    Takamiya, K. and Dutton, P.L. (1977) FEBS Letts. 80, 279–284.CrossRefGoogle Scholar
  25. 25.
    Schoenfeld, M., Montal, M. and Feher, G. (1979) Proc. Natl. Acad. Sci. USA 76, 6351–6355.ADSCrossRefGoogle Scholar
  26. 26.
    Packham, N.K., Dutton, P.L. and Mueller, P. (1980) FEBS Letts. 110, 101–106.CrossRefGoogle Scholar
  27. 27.
    Packham, N.K., Dutton, P.L. and Mueller, P. (1982) Biophys. J. 37, 465–473.ADSCrossRefGoogle Scholar
  28. 28.
    Gopher, A., Blatt, Y., Okamura, M.Y. and Feher, G. (1983) Biophys. J. 41, 212a.Google Scholar
  29. 29.
    Feher, G., Okamura, M.Y. (1984) in “Advances in Photosynthesis Research”. Edited by C. Sybesma (Kliner Academic Publishers. The Hague) 155–164.CrossRefGoogle Scholar
  30. 30.
    Gopher, A., Blatt, Y., Schoenfeld, M., Okamura, M.Y., Feher, G. and Montal, M., Biophys. J. in press.Google Scholar
  31. 31.
    Tiede, D.M., Mueller, P. and Dutton, P.L. (1982) Biochim. Biophys. Acta 681, 191–201.CrossRefGoogle Scholar
  32. 32.
    Trissl, H.W. (1983) Proc. Natl. Acad. Sci. USA 80, 7173–7177.ADSCrossRefGoogle Scholar
  33. 33.
    Tiede, D.M. (1985) Biochim. Biophys. Acta Reviews, in press.Google Scholar
  34. 34.
    Popovic, Z.D., Kovacs, G.J., Vincett, P.S. and Dutton, P.L. (1985) Chem. Phys. Lett. 116, 405–410.ADSCrossRefGoogle Scholar
  35. 35.
    Loach, P.A. and Sekura, D.L. (1968) Biochemistry 7, 2642–2649.CrossRefGoogle Scholar
  36. 36.
    Wraight, C.A. and Clayton, R.K. (1973) Biochim. Biophys. Acta 333, 246–260.CrossRefGoogle Scholar
  37. 37.
    Cho, H.M., Mancino, L.J. and Blakenship, R.E. (1984) Biophys. J. 45, 455–461.CrossRefGoogle Scholar
  38. 38.
    Clayton, R.K. and Wang, T.R., (1971) Methods Enzymol. 23A, 696–704.Google Scholar
  39. 39.
    Okamura, L., Sterner, A. and Feher, G. (1974) Biochemistry 13, 1394–1403.CrossRefGoogle Scholar
  40. 40.
    Okamura, M.Y., Debris, R.J., Kleinfeld, D. and Feher, G. (1982) in “Function of Quinones in Energy Conserving Systems” (Trumpower, B.L., ed.) 299–317.Google Scholar
  41. 41.
    Popovic, Z.D., Kovacs, G.J., Vincett, P.S., Alegria, G., and Dutton, P.L., to be published.Google Scholar
  42. 42.
    Yang, C.C., Josefowicz, J.Y. and Alexandru, L. (1980) Thin Solid Films 74, 117–127.ADSCrossRefGoogle Scholar
  43. 43.
    Popovic, Z.D. (1983) J. Chem. Phys. 78, 1552–1558.ADSCrossRefGoogle Scholar
  44. 44.
    Momany, F.A., McGuire, R.F., Burgess, A.W. and Scheraga, H.A. (1975) J. Phys. Chem. 79, 2361–2381.CrossRefGoogle Scholar
  45. 45.
    Van Krevelen, D.W. and Hoftyzer, P.J. (1976) “Properties of Polymers”, 2nd Edition. Elsevier, Amsterdam, p. 231.Google Scholar
  46. 46.
    Popovic, Z.D., Kovacs, G.J., Vincett, P.S., Alegria, G., and Dutton, P.L., to be published.Google Scholar
  47. 47.
    Gunner, M.R., Woodbury, N., Parson, W.W., Dutton, P.L. Conference on Protein Structure posterbook (1985) p. 11.Google Scholar
  48. 48.
    Tiede, D.M. and Dutton, P.L. (1981) Biochim. Biophys. Acta 637, 278–290.CrossRefGoogle Scholar
  49. 49.
    Redi, M. and Hopfield, J.J. (1980) J. Chem. Phys. 72, 6651.ADSCrossRefGoogle Scholar
  50. 50.
    Jortner, J. (1980) J. Am. Chem. Soc. 102, 6676–6686.CrossRefGoogle Scholar
  51. 51.
    Rackovsky, S. and Scher, H. (1982) Biochim. Biophys. Acta 681, 152–160.CrossRefGoogle Scholar
  52. 52.
    Marcus, R.A. (1964) Annu. Rev. Phys. Chem. 15, 155–196.ADSCrossRefGoogle Scholar
  53. 53.
    Beitz, J.V. and Miller, J.R. (1979) J. Chem. Phys. 71, 4579–4595.ADSCrossRefGoogle Scholar
  54. 54.
    Gunner, M.R., Liang, Y., Nagus, D.K., Hochstrasser, R.M. and Dutton, P.L. (1982) Biophys. J. 37, 226a.Google Scholar
  55. 55.
    Gunner, M.R., Tiede, D.M., Prince, R.C. and Dutton, P.L. (1982) in “Functions of Quinones in Energy Conversion Systems”, B.L. Trumpower, ed., Academic Press, New York and London, 271–276.Google Scholar
  56. 56.
    Clayton, R.K. and Devault, D. (1972) Photochem. Photobiol. 15, 165–175.CrossRefGoogle Scholar
  57. 57.
    D. Kleinfeld (1984), Ph.D. thesis, University of California, San Diego, pp. 238–246.Google Scholar

Copyright information

© Springer Science+Business Media New York 1987

Authors and Affiliations

  • Z. Popovic
    • 1
  • G. Kovacs
    • 1
  • P. Vincett
    • 1
  • G. Alegria
    • 2
  • P. L. Dutton
    • 2
  1. 1.Xerox Research Centre of CanadaMississaugaCanada
  2. 2.Department of Biochemistry and BiophysicsUniversity of PennsylvaniaPhiladelphiaUSA

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