Skip to main content
SpringerLink
Log in

Viral Replication, Translation, and Assembly of Nepoviruses

  • Chapter
Current Topics in Vector Research

Part of the book series: Current Topics in Vector Research ((VECTOR,volume 3))

  • 84 Accesses

Abstract

The first recognition that nepoviruses are multicomponent was by Stace-Smith et al. (40). Partially purified tobacco ringspot virus (TRSV) was shown to have three zones in sucrose density gradients (SDG), with S values of 53, 94, and 128, respectively, for top (T), middle (M), and bottom (B) components. These components were shown to be serologically identical. Electron microscopy revealed that all components were spherical, ~28 mm in diameter, and that the T particles were empty shells. Further, the B component was shown to contain 42% RNA and was infectious. Then, in 1966, Diener and Schneider (6) were the first to recognize the existence of two species of RNA in TRSV virions (RNA-1 and RNA-2), by separating them in SDG. They found that the single RNA species from M was noninfectious and that the two RNA species from the B component contained an infectious RNA (32S) and a noninfectious RNA (24S) (RNA-1 and RNA-2), with the noninfectious RNA being in much greater proportion than the infectious RNA. The noninfectious RNA from B sedimented at the same rate as that from the M component. They hypothesized that two smaller, noninfectious RNAs might join to form the longer, infectious RNA. In 1972, Murant et al. (20) demonstrated that raspberry ringspot virus (RRV) (a nepovirus of European origin) had T, M, and B components with S values of 52, 92, and 130, respectively, and containing 0, 30, and 44% single-stranded RNA.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 39.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 54.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Brawerman, G., 1974, Eukaryotic messenger RNA, Annu. Rev. Biochem. 43:621–642.

    Article  PubMed  CAS  Google Scholar 

  2. Bruening, G., 1977, Plant covirus systems: Two component systems, in: H. Fraenkel-Conrat and R. R. Wagner (eds.), Comprehensive Virology, Vol. II Plenum Press, New York, pp. 55–141.

    Google Scholar 

  3. Chu, P.W.G., and Francki, R.LB., 1979, The chemical subunit of tobacco ringspot virus coat protein, Virology 93:398–412.

    Article  PubMed  CAS  Google Scholar 

  4. Davies, D.L., and Clark, M.F., 1983, A satellite-like nucleic acid of arabis mosaic virus associated with hop nettlehead disease, Ann. Appl. Bioi. 103:439–448.

    Article  Google Scholar 

  5. Davis, J.W. and Hull, R., 1982, Genome expression of plant positive-strand RNA viruses, J. Gen. Virol. 61:1–14.

    Article  Google Scholar 

  6. Diener, T.O., and Schneider, I.R., 1966, The two components of tobacco ringspot virus nucleic acid: Origin and properties. Virology 29:100–105.

    Article  PubMed  CAS  Google Scholar 

  7. Doel, T.R., 1975, Comparative properties of type, nasturtium ringspot and petunia ringspot strains of broad bean wilt virus, J. Gen. Virol. 26:95–108.

    Article  Google Scholar 

  8. Fritsch, C., Mayo, M.A., and Murant, A.F., 1978, Translation of satellite RNA of tomato black ring virus in vitro and in tobacco protoplasts, J. Gen.Virol. 40:587–593.

    Article  CAS  Google Scholar 

  9. Fritsch, C., Mayo, M.A., and Murant, A.F., 1980, Translation products of genome and satellite RNAs of tomato black ring virus, J. Gen. Virol. 46:381–390.

    Article  CAS  Google Scholar 

  10. Gergerich, R.C., Asher, Jr., J.H., and Ramsdell, D.C., 1983, A comparison of some serological and biological properties of seven isolates of tobacco ringspot virus, Phytopathol. Z. 107:292–300.

    Article  Google Scholar 

  11. Harrison, B.D., and Barker, H., 1978, Protease-sensitive structure needed for infectivity of nepovirus RNA, J. Gen. Virol. 40:711–715.

    Article  Google Scholar 

  12. Harrison, B.D., and Murant, A.F., 1977, Nepovirus group, CMI/AAB Descriptions of Plant Viruses, No. 185, Commonwealth Mycological Institute/Association of Applied Biologists, Kew, Surrey, England.

    Google Scholar 

  13. Harrison, B.D., Murant, A.F., and Mayo, M.A., 1972, Evidence for two functional RNA species in raspberry ringspot virus, J. Gen. Virol. 16:339–348.

    Article  CAS  Google Scholar 

  14. Kiefer, M.C., Daubert, S.D., Schneider, I.R., and Bruening, G., 1982, Multimeric forms of satellite of tobacco ringspot virus RNA, Virology 121:262–273.

    Article  PubMed  CAS  Google Scholar 

  15. Mayo, M.A., Murant, A.F., and Harrison, B.D., 1971, New evidence for the structure of nepoviruses, J. Gen. Virol. 12:175–178.

    Article  PubMed  CAS  Google Scholar 

  16. Mayo, M.A., Barker, H., and Harrison, B.D., 1979a, Polyadenylate in the RNA of five nepoviruses, J. Gen. Virol. 43:603–610.

    Article  CAS  Google Scholar 

  17. Mayo, M.A., Barker, H., and Harrison, B.D., 1979b, Evidence for a protein co-valently linked to tobacco ringspot virus RNA, J. Gen. Virol. 43:735–740.

    Article  CAS  Google Scholar 

  18. Mayo, M.A., Barker, H., and Harrison, B.D., 1982, Specificity and properties of the genome-linked proteins ofnepoviruses, J. Gen. Virol 59:149–162.

    Article  CAS  Google Scholar 

  19. Murant, A.F., and Mayo, M.A., 1982, Satellites of plant viruses, Annu. Rev. Phytopathol. 20:49–70.

    Article  CAS  Google Scholar 

  20. Murant, A.F., Mayo, M.A., Harrison, B.D., and Goold, R.A., 1972, Properties of virus and RNA components of raspberry ringspot virus, J. Gen. Virol. 16:327–338.

    Article  CAS  Google Scholar 

  21. Murant, A.F., Taylor, M., Duncan, G.H., and Raschke, J.H., 1981, Improved estimates of molecular weight of plant virus RNA by agarose gel electrophoresis and electron microscopy after denaturation with glyoxol, J. Gen. Virol. 53:321–332.

    Article  CAS  Google Scholar 

  22. Owens, R.A., and Diener, T.O., 1982, RNA intermediates in potato spindle tuber viroid replication, Proc. Natl. Acad. Sci. USA Cell Biol. 79:113–117.

    Article  CAS  Google Scholar 

  23. Peden, K.W.C., May, J.T., and Symons, R.H., 1972, A comparison of two plant virus-induced RNA polymerases, Virology 47:498–501.

    Article  PubMed  CAS  Google Scholar 

  24. Pelham, H., 1979, Synthesis and proteolytic processing of cowpea mosaic virus proteins in reticulocyte lysates, Virology 96:463–477.

    Article  PubMed  CAS  Google Scholar 

  25. Randles, J.W., Harrison, B.D., Murant, A.F., and Mayo, M.A., 1977, Packaging and biological activity of the two essential RNA species of tomato black ring virus, J. Gen. Virol. 36:187–194.

    Article  Google Scholar 

  26. Rezaian, M.A., and Francki, R.I.E., 1973, Replication of tobacco ringspot virus. I. Detection of a low molecular weight double stranded RNA from infected plants, Virology 56:238–249.

    Article  PubMed  CAS  Google Scholar 

  27. Rezaian, M.A., and Francki, R.I.E., 1974, Replication of tobacco ringspot virus. II. Differences in nucleotide sequences between the viral RNA components, Virology 59:275–280.

    Article  PubMed  CAS  Google Scholar 

  28. Rezaian, M.A., Francki, R.I.B., Chu, P.W.G., and Hatta, T., 1976, Replication of tobacco ringspot virus. III.Site of virus synthesis in cucumber cotyledons, Virology 74:481–488.

    Article  PubMed  CAS  Google Scholar 

  29. Robinson, D.J., Basker, H., Harrison, B.D., and Mayo, M.A., 1980, Replication of RNA-l of tomato black ring virus independently of RNA-2, J. Gen. Virol. 51:317–326.

    CAS  Google Scholar 

  30. Schneider, I.R., 1970, Interaction between several viruses and the satellitelike virus of tobacco ringspot virus, Phytopathology 60: 1312 (abstract).

    Article  Google Scholar 

  31. Schneider, I.R., 1971, Characteristics ofa satellite-like virus of tobacco ringspot virus, Virology 45:108–122.

    Article  PubMed  CAS  Google Scholar 

  32. Schneider, I.R., 1978, Defective plant viruses, in: J.A. Romberger et al. (eds.), Beltsville Symposia in Agricultural Research Allanheld, Osmun, Montclair, New Jersey, pp. 201–219.

    Google Scholar 

  33. Schneider, I.R., and Thompson, S.M., 1977, Double-stranded nucleic acids found in tissue infected with the satellite of tobacco ringspot virus, Virology 78:453–462.

    Article  PubMed  CAS  Google Scholar 

  34. Schneider, I.R., and White, R.M., 1976, Tobacco ringspot virus codes for the coat protein of its satellite, Virology 70:244–246.

    Article  PubMed  CAS  Google Scholar 

  35. Schneider, I.R., Hull, R., and Markham, R., 1972, Multidense satellite of tobacco ringspot virus: A regular series of components of different densities, Virology 47:320–330.

    Article  PubMed  CAS  Google Scholar 

  36. Schneider, I.R., White, R.M., and Civerolo, E.R., 1974a, Two nucleic acid-containing components of tomato ringspot virus, Virology 57:139–146.

    Article  PubMed  CAS  Google Scholar 

  37. Schneider, I.R., White, R.M., and Thompson, S.M., 1974, High molecular weight double-stranded nucleic acids from tobacco ringspot virus infected plants, Proc. Am. Phytopathol. Soc. 1:82.

    Google Scholar 

  38. Sogo, J.M., and Schneider, I.R., 1982, Electron microscopy of double stranded nucleic acids in tissue infected with the satellite tobacco ringspot virus, Virology 117:401–415.

    Article  PubMed  CAS  Google Scholar 

  39. Sogo, J.M., Schneider, I.R., and Koller, T., 1974, Size determination by electron microscopy of the RNA of tobacco ringspot satellite virus, Virology 57:459–466.

    Article  PubMed  CAS  Google Scholar 

  40. Stace-Smith, R., Reichmann, M.E., and Wright, N.S., 1965, Purification and properties of tobacco ringspot virus and two RNA-deficient components, Virology 25:487–494.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Botany and Plant Pathology, Michigan State University, 48824, East Lansing, Michigan, USA

    Donald C. Ramsdell

Authors
  1. Donald C. Ramsdell
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Virus-Vector Laboratory, Department of Entomology, Texas A&M University, 77843, College Station, Texas, USA

    Kerry F. Harris

Rights and permissions

Reprints and permissions

Copyright information

© 1987 Springer-Verlag New York Inc.

About this chapter

Cite this chapter

Ramsdell, D.C. (1987). Viral Replication, Translation, and Assembly of Nepoviruses. In: Harris, K.F. (eds) Current Topics in Vector Research. Current Topics in Vector Research, vol 3. Springer, New York, NY. https://doi.org/10.1007/978-1-4612-4688-6_6

Download citation

  • DOI: https://doi.org/10.1007/978-1-4612-4688-6_6

  • Publisher Name: Springer, New York, NY

  • Print ISBN: 978-1-4612-9115-2

  • Online ISBN: 978-1-4612-4688-6

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation