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Endothelial-Dependent Binding of [125I]-Bolton-Hunter Substance P in Blood Vessels

  • J. A. Stephenson
  • R. J. Summers
  • E. Burcher
Conference paper

Abstract

Substance P is one of the most potent vasodilators known [1] and the vasodilatation in some dog, cat and rabbit arteries is endothelium-dependent [2]. Endothelium-dependent relaxation by Substance P has since been shown in rings of dog common carotid arteries [3], pig and dog coronary arteries [4] and in vivo in dog femoral artery [5]. This vasodilatation may be mediated by release of endothelium-derived relaxing factor (EDRF) [2]. In the study described here, we have used the techniques of in vitro radioligand binding and autoradiography [6] to detect the localization of [125I] Bolton-Hunter substance P (BHSP) to sections of blood vessels with the endothelium intact and to similar preparations with the endothelium removed.

Keywords

Methylene Blue Intact Endothelium Autoradiographic Localization Bovine Aorta Light Microscopic Autoradiography 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag New York Inc. 1987

Authors and Affiliations

  • J. A. Stephenson
    • 1
  • R. J. Summers
    • 1
  • E. Burcher
    • 2
  1. 1.Department of PharmacologyUniversity of MelbourneVictoriaUSA
  2. 2.Department of Biological and Health SciencesDeakin UniversityVictoriaAustralia

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