Endothelial-Dependent Binding of [125I]-Bolton-Hunter Substance P in Blood Vessels
Substance P is one of the most potent vasodilators known  and the vasodilatation in some dog, cat and rabbit arteries is endothelium-dependent . Endothelium-dependent relaxation by Substance P has since been shown in rings of dog common carotid arteries , pig and dog coronary arteries  and in vivo in dog femoral artery . This vasodilatation may be mediated by release of endothelium-derived relaxing factor (EDRF) . In the study described here, we have used the techniques of in vitro radioligand binding and autoradiography  to detect the localization of [125I] Bolton-Hunter substance P (BHSP) to sections of blood vessels with the endothelium intact and to similar preparations with the endothelium removed.
KeywordsMethylene Blue Intact Endothelium Autoradiographic Localization Bovine Aorta Light Microscopic Autoradiography
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