Abstract
Antigen-antibody interactions such as they occur in the course of adaptive immune responses in vertebrates are highly specific. Since proteins are amongst the most frequent and diverse antigens encountered by immune systems, a large number of immunochemical studies have been made using model proteins to characterize the precise interactions between antigens and antibodies in the course of immune responses (reviewed in Benjamin et al., 1). With the aim of attaining atomic resolution in the description of those interactions, we have chosen hen egg-white lysozyme (HEL) as an antigen. HEL is a small, thoroughly characterized protein, easy to obtain and purify and very immunogenic in the BALB/c strain of mice used in this work. HEL variants, with amino acid sequence differences scattered all along their sequences occur in the eggs of different gallinaceous birds. The three-dimensional structure of HEL has been determined (2) as well as those of turkey (3), human (4) and phage T4 lysozyme (5).
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Benjamin, D.C., Berzofsky, J.A., East, I.J., Gurd, F. R.N., Hannum, C., Leach, S.J., Margoliash, E., Michael, J.G., Miller, A., Prager, E.M., Reichlin, M., Sercarz, E.E., Smith-Gill, S.J., Todd, P.E. and Wilson, A.C. (1984) Ann. Rev. Immunol., 2, 67–101.
Blake, C.C.F., Mair, G.A., North, A.C.T., Phillip, D. C. and Sarma, V.R. (1967) Proc. Roy. Soc. London B, 167, 365–377.
Sarma, R. and Bott, R. (1977) J. Mol. Biol., 113, 555–565.
Artymiuk, P.J. and Blake C.C.F. (1981) J. Mol. Biol.152, 737–762.
Matthews, B.W., Grutter, M.G., Anderson, W.F. and Remington, S.J. (1981) Nature 290, 334–335.
Köhler, G. and Milstein, C. (1975) Nature 256, 495–497.
Harper, M., Lema, F., Boulot, G. and Poljak, R.J. (1986) Mol. Immunol., in press.
Mariuzza, R.A., Jankovic, D.L.J., Boulot, G., Amit, A.G., Saludjian, P., Le Guern, A., Mazié, J.C. and Poljak, R.R. (1983) J. Mol. Biol. 170, 1055–1058.
Jones, T.A. (1978) J. App. Cryst. 11, 268–272.
Verhoeyen, M., Berek, C., Jarvis, J.M., Winter, G. (1986) Personal communication.
Luzzati, V. (1952) Acta Cryst. 5, 802–808.
Amit, A.G., Mariuzza, R.A., Phillips, S.E.V. and Poljak, R.J. (1986) Science 233, 747–753.
Amit, A.G., Mariuzza, R.A., Phillips, S.E.V. and Poljak, R.J. (1985) Nature 313, 156–158.
Chothia, A.M., Lesk, A.M., Levitt, M., Amit, A.G., Mariuzza, R.A., Phillips, S.E.V. and Poljak, R.J. ((1986) Science 233, 755–757.
Kabat, E.A., Wu, T.T., Bilofsky, H., Reid-Miller, M. and Perry, H. (1983) in: Sequences of Proteins of Immunological Interest. U.S. Department of Health and Public Services: 1.
Tonegawa, S. (1983) Nature 302, 575–581.
Honjo, T. (1983) Ann. Rev. Immunol. 1, 499–528.
Lee, B. and Richards, F.M. (1971) J. Mol. Biol. 55,379–400.
Chothia, C. and Janin, J. (1975) Nature 256, 705–708.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1987 The Humana Press Inc.
About this chapter
Cite this chapter
Amit, A.G., Harper, M., Lema, F., Mariuzza, R.A., Phillips, S.E.V., Poljak, R.J. (1987). Fine Specificity of Monoclonal Anti-Lysozyme Antibodies and the Three-Dimensional Structure of a Lysozyme-Antibody Complex. In: Chaiken, I., Chiancone, E., Fontana, A., Neri, P. (eds) Macromolecular Biorecognition. Experimental Biology and Medicine, vol 19. Humana Press. https://doi.org/10.1007/978-1-4612-4600-8_18
Download citation
DOI: https://doi.org/10.1007/978-1-4612-4600-8_18
Publisher Name: Humana Press
Print ISBN: 978-1-4612-8944-9
Online ISBN: 978-1-4612-4600-8
eBook Packages: Springer Book Archive