Transition of Chromatin from the “10 nm” Lower Order Structure, to the “30 nm” Higher Order Structure, as Followed by Small Angle X-Ray Scattering

  • K. O. Greulich
  • E. Wachtel
  • J. Ausio
  • D. Seger
  • H. Eisenberg
Chapter
Part of the Experimental Biology and Medicine book series (EBAM, volume 19)

Abstract

Double helical Watson-Crick B DNA is a stiff macro-molecule which, in accordance with its basic structure (1), should proceed in free space in strictly linear fashion. Though abnormally migrating DNA has recently been discovered (2), and naturally curved DNA can presently be considered a highly fashionable topic of investigation (3), it must be considered an important exception, rather than the general rule. Still, even unrestricted in dilute solution, DNA is less rigid than could be gathered from its multiply fixed hydrogen bonded structure, and bends considerably as a result of disordering due to thermal Brownian motion. To describe the coiling behavior of DNA quantitatively the persistent chain or wormlike coil of Kratky and Porod (4) has been introduced. In this description the persistence length a equals about 40 nm in 0.2M NaCl (5), corresponding to a curvature radius of this order. Short DNA chains are almost rodlike in solution.

Keywords

Sucrose EDTA Sedimentation MgCl2 Fractionation 

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References

  1. 1.
    Watson, J.D. and Crick, F.H.C. (1953) Nature 171, 737–738.PubMedCrossRefGoogle Scholar
  2. 2.
    Simpson, L., Simpson, A.M., Kidane, G., Livingston, L. and Spithill, T.W. (1980) Am. J. Trop. Med. Hyg. 29(5) Suppl., 1053–1063.PubMedGoogle Scholar
  3. 3.
    Eisenberg, H. (1986) TIBS 11, 350–351.Google Scholar
  4. 4.
    Kratky, O. and Porod, G. (1949) Rec. Trav. Chim. Pays-Bas 68, 1106–1122.CrossRefGoogle Scholar
  5. 5.
    Kam, Z., Borochov, N. and Eisenberg, H. (1981) Biopolymers 20, 2671–2690.PubMedCrossRefGoogle Scholar
  6. 6.
    Chan, P.T., Ohmori, H., Tomizawa, J. and Lebowitz, J. (1985) J. Biol. Chem. 260, 8925–8935.PubMedGoogle Scholar
  7. 7.
    Richmond, T.J., Finch, J.T., Rushton, B., Rhodes, D. and Klug, A. (1984) Nature (London) 311, 532–537.CrossRefGoogle Scholar
  8. 8.
    Kornberg, A. (1980) DNA Replication, W.H. Freeman and Company, San Francisco, CA.Google Scholar
  9. 9.
    Bradbury, E.M., Maclean, N. and Matthews, H.R. (1981) DNA, Chromatin and Chromosomes. John Wiley and Sons, Inc., New York.Google Scholar
  10. 10.
    Finch, J.T. and Klug, A. (1976) Proc. Natl. Acad. Sci, USA 73, 1897–1901.PubMedCrossRefGoogle Scholar
  11. 11.
    Staynov, D.Z. (1983) Int. J. Biol. Macromol. (, 3–9.Google Scholar
  12. 12.
    Felsenfeld, G. and McGhee, J.D. (1986) Cell 44, 375–377.PubMedCrossRefGoogle Scholar
  13. 13.
    Widom, J. and Klug, A. (1985) Cell 43, 207–213.PubMedCrossRefGoogle Scholar
  14. 14.
    Widom, J., Finch, J.T. and Thomas, J.O. (1985) EMB0 J. 4, 3189–3194.Google Scholar
  15. 15.
    Williams, S.P., Athey, B.D., Muglia, L.J., Schappe, R.S., Gough, A.H. and Langmore, J.P. (1986) Biophys. J. 49, 233–248.PubMedCrossRefGoogle Scholar
  16. 16.
    Perez-Grau, L., Bordas, T. and Koch, M.H.J. (1984) Nucl. Acids Res. 12, 2987–2996.PubMedCrossRefGoogle Scholar
  17. 17.
    Bordas, J., Perez-Grau, L., Koch, M.H.J., Vega, M.C. and Nave, C. (1986) Eur. Biophys. J. 13, 157–174.PubMedCrossRefGoogle Scholar
  18. 18.
    Bordas, J., Perez-Grau, L., Koch, M.H.J., Vega, M.C. and Nave C. (1986) Eur. Biophys. J. 13, 175–186.PubMedCrossRefGoogle Scholar
  19. 19.
    Ausio, J., Borochov, N., Seger, D. and Eisenberg, H. (1984) J. Mol. Biol. 177, 373–398.PubMedCrossRefGoogle Scholar
  20. 20.
    Suau, P., Bradbury, E.M. and Baldwin, J.P. (1979) Eur. J. Biochem. 97, 593–602.PubMedCrossRefGoogle Scholar
  21. 21.
    Sperling, L. and Klug, A. (1977) J. Mol. Biol. 112, 253–263.PubMedCrossRefGoogle Scholar
  22. 22.
    Fulmer, A.W. and Bloornfield, V.A. (1982) Biochemistry 21, 985–992.PubMedCrossRefGoogle Scholar
  23. 23.
    Compton, J.L., Bellard, M. and Chambon, P. (1976) Proc. Natl. Acad. Sci. USA 73, 4382–4386.PubMedCrossRefGoogle Scholar
  24. 24.
    Greulich, K.O., Wachtel, E., Ausio, J., Seger, D. and Eisenberg, H. (1987) J. Mol. Biol, in press.Google Scholar
  25. 25.
    Borochov, N., Ausio, J. and Eisenberg, H. (1984) Nucl. Acids Res. 12, 3089–3096.PubMedCrossRefGoogle Scholar
  26. 26.
    Sanders, M.M. (1978) J. Cell. Biol. 79, 97–109.PubMedCrossRefGoogle Scholar
  27. 27.
    Hollandt, H., Notbohm, H., Riedel, F. and Harbers, E. (1979) Nucl. Acids Res. 6, 2017, 2027.CrossRefGoogle Scholar
  28. 28.
    Damaschun, H., Damaschun, G., Pospelov, V.A. and Vorob’ev, V.I. (1980) Molec. Biol. Rep. 6, 185–191.CrossRefGoogle Scholar
  29. 29.
    Brust, R. and Harbers, E. (1981) Eur. J. Biochem. 117, 609–614.PubMedCrossRefGoogle Scholar
  30. 30.
    Reich, M.H. (1982) Ph.D. thesis, The Weizmann Institute of Science, Rehovot, Israel.Google Scholar
  31. 31.
    McGhee, J.D., Rau, D.C., Charney, E. and Felsenfeld, G. (1980) Cell 22, 87–96.PubMedCrossRefGoogle Scholar
  32. 32.
    Allan, J., Staynov, D.Z. and Gould, H. (1980) Proc. Natl. Acad. Sci. USA 77, 885–889.PubMedCrossRefGoogle Scholar
  33. 33.
    Sperling, L. and Tardieu, A. (1976) FEBS Letters 64, 89–91.PubMedCrossRefGoogle Scholar
  34. 34.
    Thoma, F., Koller, Th. and Klug, A. (1979) J. Cell. Biol. 83, 403–427.PubMedCrossRefGoogle Scholar
  35. 35.
    Woodcock, C.L.F., Frado, L.L.Y. and Rattner, J.B. (1984) J. Cell. Biol. 99, 42–52.PubMedCrossRefGoogle Scholar
  36. 36.
    Yamakawa, H. and Fujii, M. (1973) Macromolecules 6, 407–415.CrossRefGoogle Scholar
  37. 37.
    Timasheff, S.N., Witz, J. and Luzzati, V. (1981) Biophys. J. 1, 525–537.CrossRefGoogle Scholar
  38. 38.
    Glatter, O. (1979) J. App. Crystallogr. 12, 166–175.CrossRefGoogle Scholar
  39. 39.
    Kirste, R.G. (1967) in: Small Angle X-Ray Scattering (Brumberger, H. ed.) Gordon and Breach, New York, pp. 33–61.Google Scholar
  40. 40.
    Kirste, R.G. and Oberthür, R.C. (1982) in: Small Angle X-Ray Scattering (Glatter, O. and Kratky, O., eds.) Academic Press, London, pp. 387–431.Google Scholar

Copyright information

© The Humana Press Inc. 1987

Authors and Affiliations

  • K. O. Greulich
    • 1
  • E. Wachtel
    • 1
  • J. Ausio
    • 1
  • D. Seger
    • 1
  • H. Eisenberg
    • 1
  1. 1.Polymer DepartmentThe Weizmann Institute of ScienceRehovotIsrael

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