Role of Glycosylation Inhibiting Factor (GIF), a Phospholipase Inhibitory Protein, in the Generation of Antigen-Specific Suppressor T Cells
Previous experiments on the isotype-specific regulation of the IgE antibody response revealed T cell factors which have affinity for IgE and either enhance or suppress the IgE response (1). The major differences between the IgE-potentiating factors (PF) and IgE-suppressive factors (SF) are carbohydrate moieties in the molecules. The IgE-potentiating factors bind to lentil lectin and Con A, suggesting that the factors contain N-linked, mannose-rich oligosaccharide. In contrast, IgE-suppressive factors have no affinity for these lectins but bound to peanut agglutinin (2). Recent experiments in collaboration with Drs. Moore and Martens in DNAX Institute of Molecular Biology indicate that IgE-PF and IgE-SF share a common structural gene (3). Transfection of COS 7 monkey kidney cells with the cDNA clone 8.3, which encodes the rodent IgE-binding factor (4) resulted in the formation of IgE-PF, whereas the transfection of the same cells with the same cDNA clone in the presence of tunicamycin resulted in the formation of IgE-SF.
KeywordsHPLC Filtration Carbohydrate Lipase Polypeptide
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