Induction of Differentiation of 3T3-L1 Fibroblasts to Adipocytes by 3-Deazaadenosine and Insulin

  • Peter K. Chiang
  • Nesbitt D. Brown
  • Felipe N. Padilla
  • Richard K. Gordon
Part of the Experimental Biology and Medicine book series (EBAM, volume 17)


3-Deazaadenosine (dzAdo), an analog of adenosine, is a potent proximal inhibitor of S-adenosylmethionine-dependent transmethylation reactions (1–3). A variety of transmethylation reactions have been shown to be inhibited by dzAdo because of its ability to act as an alternative substrate and also as an inhibitor of S-adenosylhomocysteine hydrolase, which hydrolyzes S-adenosylhomocysteine to adenosine and L-homocysteine. A unique feature of S-adenosylhomocysteine hydrolase is that it can synthesize a novel S-nucleosidylhomocysteine analog by condensing a nucleoside with L-homocysteine because the equilibrium of the reaction favors the synthetic direction (1–5). When dzAdo is administered to cells or animals, the net result is the accumulation of S-adenosylhomocysteine and S-3-deazaadenosylhomocysteine, both of which are known potent inhibitors of transmethylation reactions (6–11).


Epidermal Growth Factor Protein Glycosylation Alternative Substrate Total Triglyceride Fibroblast Differentiation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© The Humana Press Inc. 1987

Authors and Affiliations

  • Peter K. Chiang
    • 1
  • Nesbitt D. Brown
    • 1
  • Felipe N. Padilla
    • 1
  • Richard K. Gordon
    • 1
  1. 1.Department of Applied BiochemistryWalter Reed Army Institute of ResearchUSA

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