Polyclonal Activation of B Lymphocytes after EB Virus Infection
CD23 (Blast-2) is a 45k protein which is a B cell activation antigen appearing early after EB virus infection1 It is then shed from the surface as a 33k protein (s-Blast-2) which is thought to act as a growth factor for activated B cells.2 Separation of CD23+ and CD23- B cell fractions shortly after infection gives a population of immortalised cells (CD23+) and a non-immortalised population (CD23-)3. CD 20 is a 35k protein present on all B cells. Anti-CD20-antibody, B1, inhibits activation of B cells by pokeweed mitogen but not by the phorbol ester, TPA4. We have studied the effect of anti-CD23 antibody (MHM6) and anti-CD20-antibody, (B1) on B cell activation, immortalisation and immunoglobulin (Ig) production by EB virus.
KeywordsCell Fraction Phorbol Ester Negative Cell Graduate Medical School Pokeweed Mitogen
- 2.Swendeman S.L. and Thorley-Lawson D.A. Abstract at the 11th INTERNATIONAL HERPES VIRUS MEETING, LEEDS, JULY 1985.Google Scholar