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Polyclonal Activation of B Lymphocytes after EB Virus Infection

  • T. Azim
  • J. Golay
  • K. Lam
  • D. H. Crawford
Part of the Experimental Biology and Medicine book series (EBAM, volume 15)

Abstract

CD23 (Blast-2) is a 45k protein which is a B cell activation antigen appearing early after EB virus infection1 It is then shed from the surface as a 33k protein (s-Blast-2) which is thought to act as a growth factor for activated B cells.2 Separation of CD23+ and CD23- B cell fractions shortly after infection gives a population of immortalised cells (CD23+) and a non-immortalised population (CD23-)3. CD 20 is a 35k protein present on all B cells. Anti-CD20-antibody, B1, inhibits activation of B cells by pokeweed mitogen but not by the phorbol ester, TPA4. We have studied the effect of anti-CD23 antibody (MHM6) and anti-CD20-antibody, (B1) on B cell activation, immortalisation and immunoglobulin (Ig) production by EB virus.

Keywords

Cell Fraction Phorbol Ester Negative Cell Graduate Medical School Pokeweed Mitogen 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. 1.
    Thorley-Lawson D.A., et al. J.Immunol. 134, No.5 3007 (1985).PubMedGoogle Scholar
  2. 2.
    Swendeman S.L. and Thorley-Lawson D.A. Abstract at the 11th INTERNATIONAL HERPES VIRUS MEETING, LEEDS, JULY 1985.Google Scholar
  3. 3.
    Thorley-Lawson D.A., et al. J.Exp.Med. 162, 45 (1985).PubMedCrossRefGoogle Scholar
  4. 4.
    Tedder T.F., et al. Eur.J.Immunol. 16:881 (1986).PubMedCrossRefGoogle Scholar

Copyright information

© The Humana Press Inc. 1987

Authors and Affiliations

  • T. Azim
    • 1
  • J. Golay
    • 2
  • K. Lam
    • 2
  • D. H. Crawford
    • 2
  1. 1.Dept. of HaematologyUCL Medical SchoolLondonUK
  2. 2.Dept. of VirologyRoyal Post-Graduate Medical SchoolLondonUK

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