Polyclonal Activation of B Lymphocytes after EB Virus Infection

  • T. Azim
  • J. Golay
  • K. Lam
  • D. H. Crawford
Part of the Experimental Biology and Medicine book series (EBAM, volume 15)

Abstract

CD23 (Blast-2) is a 45k protein which is a B cell activation antigen appearing early after EB virus infection1 It is then shed from the surface as a 33k protein (s-Blast-2) which is thought to act as a growth factor for activated B cells.2 Separation of CD23+ and CD23- B cell fractions shortly after infection gives a population of immortalised cells (CD23+) and a non-immortalised population (CD23-)3. CD 20 is a 35k protein present on all B cells. Anti-CD20-antibody, B1, inhibits activation of B cells by pokeweed mitogen but not by the phorbol ester, TPA4. We have studied the effect of anti-CD23 antibody (MHM6) and anti-CD20-antibody, (B1) on B cell activation, immortalisation and immunoglobulin (Ig) production by EB virus.

Keywords

Thymidine Phorbol 

References

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    Thorley-Lawson D.A., et al. J.Immunol. 134, No.5 3007 (1985).PubMedGoogle Scholar
  2. 2.
    Swendeman S.L. and Thorley-Lawson D.A. Abstract at the 11th INTERNATIONAL HERPES VIRUS MEETING, LEEDS, JULY 1985.Google Scholar
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    Thorley-Lawson D.A., et al. J.Exp.Med. 162, 45 (1985).PubMedCrossRefGoogle Scholar
  4. 4.
    Tedder T.F., et al. Eur.J.Immunol. 16:881 (1986).PubMedCrossRefGoogle Scholar

Copyright information

© The Humana Press Inc. 1987

Authors and Affiliations

  • T. Azim
    • 1
  • J. Golay
    • 2
  • K. Lam
    • 2
  • D. H. Crawford
    • 2
  1. 1.Dept. of HaematologyUCL Medical SchoolLondonUK
  2. 2.Dept. of VirologyRoyal Post-Graduate Medical SchoolLondonUK

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