EBNA-1 Binds Specifically to Plasmids Containing a Synthetic 29 BP Binding Site

  • Gregory Milman
Part of the Experimental Biology and Medicine book series (EBAM, volume 15)

Abstract

The carboxyl-third of EBNA-1 encoded in the Epstein-Barr virus (EBV) BamH1 K-restriction fragment was synthesized in bacteria (28K-EBNA). This bacterially synthesized peptide specifically binds to clustered EBV DNA sequence repeats in the Ori-P region required for plasmid maintenance [1]. To elucidate the binding properties, a synthetic DNA binding site
  • 5′ GATCTAGGATAGCAT|ATGCTACCCCGGGG 3′

  • 3′ ATCCTATCGTA|TACGATGGGGCCCCCTAG 5′

was cloned as a monomer, dimer, or trimer into the BamH1 site of plasmid pUC8 creating Plasmids pR1, pR2, and pR3.

Keywords

Agarose 

References

  1. [1]
    Rawlins et al. Cell 42:859 (1985)PubMedCrossRefGoogle Scholar

Copyright information

© The Humana Press Inc. 1987

Authors and Affiliations

  • Gregory Milman
    • 1
  1. 1.School of Hygiene and Public HealthThe Johns Hopkins UniversityBaltimoreUSA

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