EBNA-1 Binds Specifically to Plasmids Containing a Synthetic 29 BP Binding Site
Part of the Experimental Biology and Medicine book series (EBAM, volume 15)
The carboxyl-third of EBNA-1 encoded in the Epstein-Barr virus (EBV) BamH1 K-restriction fragment was synthesized in bacteria (28K-EBNA). This bacterially synthesized peptide specifically binds to clustered EBV DNA sequence repeats in the Ori-P region required for plasmid maintenance . To elucidate the binding properties, a synthetic DNA binding site
was cloned as a monomer, dimer, or trimer into the BamH1 site of plasmid pUC8 creating Plasmids pR1, pR2, and pR3.
5′ GATCTAGGATAGCAT|ATGCTACCCCGGGG 3′
3′ ATCCTATCGTA|TACGATGGGGCCCCCTAG 5′
© The Humana Press Inc. 1987