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EBNA-1 Binds Specifically to Plasmids Containing a Synthetic 29 BP Binding Site

  • Gregory Milman
Part of the Experimental Biology and Medicine book series (EBAM, volume 15)

Abstract

The carboxyl-third of EBNA-1 encoded in the Epstein-Barr virus (EBV) BamH1 K-restriction fragment was synthesized in bacteria (28K-EBNA). This bacterially synthesized peptide specifically binds to clustered EBV DNA sequence repeats in the Ori-P region required for plasmid maintenance [1]. To elucidate the binding properties, a synthetic DNA binding site
  • 5′ GATCTAGGATAGCAT|ATGCTACCCCGGGG 3′

  • 3′ ATCCTATCGTA|TACGATGGGGCCCCCTAG 5′

was cloned as a monomer, dimer, or trimer into the BamH1 site of plasmid pUC8 creating Plasmids pR1, pR2, and pR3.

Keywords

BamH1 Site Sequence Specific Binding Relative Intensi Single Binding Site Dyad Symmetry 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. [1]
    Rawlins et al. Cell 42:859 (1985)PubMedCrossRefGoogle Scholar

Copyright information

© The Humana Press Inc. 1987

Authors and Affiliations

  • Gregory Milman
    • 1
  1. 1.School of Hygiene and Public HealthThe Johns Hopkins UniversityBaltimoreUSA

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