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Detection of EBV DNA in Nude Mouse Passaged Nasopharyngeal Carcinoma (NPC) Tissues

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Epstein-Barr Virus and Human Disease

Part of the book series: Experimental Biology and Medicine ((EBAM,volume 15))

Abstract

DNA extracted from two series of nude mouse passaged nasopharyngeal carcinoma tissues was digested with restriction erdonuclease Bam HI, then electrophoresed on agarose gel and analysed for the EBV DNA fragments by Southern hybridization with twenty-seven radioactive Bam HI DNA fragments from cloned EBV DNA of B95–8 cell line. Constant hybridization patterns were revealed in these experiments and the results suggest a stable existence of EBV DNA in these tissues even after a long time of passage. When EBV DNA Bam HI F, H, I, W and Y fragments were used as probes, extra hybridization bands were found in the autoradiographs. The significance of these divergencies is discussed.

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Author information

Authors and Affiliations

  1. Departments of Bacteriology and Pathology, College of Medicine, National Taiwan University, Taipei, Taiwan, ROC

    Jen-Yang Chen, Yueh-Hsing Ou, Tswey-Ying Hsu, Mei-Ying Liu, Teh-Shiou Huang, Lei-Ron Jan, Sheau-Wei Jang, Chun-Huey Chang & Czau-Siung Yang

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  1. Jen-Yang Chen
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  2. Yueh-Hsing Ou
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  3. Tswey-Ying Hsu
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  4. Mei-Ying Liu
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  5. Teh-Shiou Huang
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  6. Lei-Ron Jan
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  7. Sheau-Wei Jang
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  8. Chun-Huey Chang
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  9. Czau-Siung Yang
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Editor information

Editors and Affiliations

  1. National Cancer Institute, Bethesda, Maryland, USA

    P. H. Levine  & D. V. Ablashi  & 

  2. Showa University Research Institute, St. Petersburg, Florida, USA

    M. Nonoyama

  3. Georgetown University Medical Center, Washington, D C, USA

    G. R. Pearson

  4. The Ohio State University, Columbus, Ohio, USA

    R. Glaser

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© 1987 The Humana Press Inc.

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Chen, JY. et al. (1987). Detection of EBV DNA in Nude Mouse Passaged Nasopharyngeal Carcinoma (NPC) Tissues. In: Levine, P.H., Ablashi, D.V., Nonoyama, M., Pearson, G.R., Glaser, R. (eds) Epstein-Barr Virus and Human Disease. Experimental Biology and Medicine, vol 15. Humana Press. https://doi.org/10.1007/978-1-4612-4590-2_40

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  • DOI: https://doi.org/10.1007/978-1-4612-4590-2_40

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-4612-8940-1

  • Online ISBN: 978-1-4612-4590-2

  • eBook Packages: Springer Book Archive

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