Abstract
EBV DNA becomes increasingly methylated with time following cell transformation (1) while hypomethylation has been associated with expression of viral genes (2). S-adenosylhomocysteine (SAH), a product of the S-adenosylmethionine transmethylation reaction, acts as a competitive inhibitor of DNA methyltransferases (3). In the present study SAH and analogs, sinefungin (SF) and 5′-deoxy-5′-S-isobutyladenosine (SIBA), as well as 5-azacytidine, which inhibits methylation of nascent DNA (4), were utilized in studies to determine the role of DNA methylation in the regulation of Epstein-Barr virus (EBV) functions such as transformation of human peripheral blood lymphocytes (PBL) and gene expression in lymphoblastoid cell lines. SF and SIBA, but not SAH, inhibited EBV-induced transformation of human PBLs. Indirect immunofluorescence and flow cytometric analyses revealed a significant increase in viral capsid antigen expression in lymphoblastoid cell lines following exposure to the analogs SF and SIBA, but not SAH.
Keywords
- Lymphoblastoid Cell Line
- Human Peripheral Blood Lymphocyte
- Transmethylation Reaction
- Human PBLs
- HpaII Digestion
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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© 1987 The Humana Press Inc.
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Fronko, G., Henderson, E., Wu, B., Long, W. (1987). Effects of S-adenosylhomocysteine and Analogs on Epstein-Barr Virus (EBV)-Induced Transformation, EBV DNA Methylation and Gene Expression. In: Levine, P.H., Ablashi, D.V., Nonoyama, M., Pearson, G.R., Glaser, R. (eds) Epstein-Barr Virus and Human Disease. Experimental Biology and Medicine, vol 15. Humana Press. https://doi.org/10.1007/978-1-4612-4590-2_32
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DOI: https://doi.org/10.1007/978-1-4612-4590-2_32
Publisher Name: Humana Press
Print ISBN: 978-1-4612-8940-1
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