Expression and Analysis of EBV gp350 in the Yeast SaccharomycesCerevisiae
Epstein-Barr Virus (EBV) causes infectious mononucleosis and has been implicated strongly, along with essential cofactors, as a causative agent for the development of nasopharyngeal carcinoma, Burkitt’s lymphoma, and B-cell malignancies in immune-deficient patients. The two major glycoprotein components of the virion are gp350 and gp220, which are encoded by the same gene (1). They are both capable of eliciting neutralizing antibodies in vitro (2). In addition, recent data suggest that viral gp350 can be protective in a primate model (cottontop marmoset) of EBV infection (3). Thus, gp350 may be a useful immunogen for eliciting protective immunity against EBV infections. In this report, we describe the expression in yeast (Saccharomyces cerevisiae) of the secretable form of gp350, minus the membrane anchor sequence. The yeast-derived gp375 shows structural and immunological similarities to the viral gp350.
KeywordsInfectious Mononucleosis Viral Gp350 Gp350 Antibody Elicit Neutralize Antibody Immunological Similarity
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