Abstract
During the past few years, natural killer (NK) cells have attracted considerable attention because of their potential role in antitumor defenses in humans and animals (1). Recently, the cells responsible for NK activity in rodents and humans have been shown to be a subset of lymphoid cells termed large granular lymphocytes (LGL) (2,3). The mechanism of their lytic activity is still not thoroughly understood, however. Studies of the effects of metabolic inhibitors on NK activity support the stimulation-secretion model originally postulated (4,5) to explain the mechanism of killing by cytotoxic T lymphocytes (CTL) (6). In addition, Wright and Bonavida (7) have recently described a soluble factor that is produced during the interaction of mouse spleen cells and human peripheral blood lymphocytes (7–10) and that selectively lyses NK-susceptible target cells. They termed this NK cytotoxic factor (NKCF). Although several lines of correlative evidence suggest a role for NKCF in the mechanism of NK cellmediated cytolysis, a variety of important issues still remain to be settled. In the present report, NKCF was utilized in an attempt to dissect the various steps that have been postulated to be involved in NK cell-mediated lysis. Studies were performed to examine agents that have been previously demonstrated to inhibit LGL-mediated cytolysis and their effect on NKCF production by LGL and NKCF lysis of tumor target cells.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Herberman, R. B., ed. NK Cells and Other Natural Effector Cells Academic, New York (1982).
Timonen, T., Ortaldo, J. R., and Herberman, R. B. J. Exp. Med. 153, 569–582 (1981).
Reynolds, C. W., Timonen, T., and Herberman, R. B. J. Immunol. 127, 282–287 (1981).
Roder, J. C. and Haliotis, T., in Natural Cell-Mediated Immunity Against Tumors (Herberman, R. B., ed.) pp. 379–390, Academic, New York (1980).
Quan, P. C., Ishizaka, T., and Bloom, B. R. J. Immunol. 128, 1786–1791 (1982).
Henney, C. S. Transplant. Rev. 17, 37–52 (1973).
Wright, S. C. and Bonavida, B. J. Immunol. 126, 1516–1521 (1981).
Wright, S. C. and Bonavida, B. J. Immunol. 129, 433–439 (1982).
Wright, S. C., Weitzen, M. L., Kahle, R., Granger, G. A., and Bonavida, B. J. Immunol 130, 2479–2483 (1983).
Farram, E. and Targan, S. R. J. Immunol. 130, 1252–1261 (1983).
Wright, S. and Bonavida, B. Proc. Natl. Acad. Sci. USA 80(6), 1688–1692 (1983).
Bianca, I., Herberman, R. B., and Ortaldo, J. R. Natl. Immun. Cell Growth Reg. 4, 48–59 (1985).
Ortaldo, J. R., Bianca, I., and Herberman, R. B., in Mechanisms of Cell-Mediated Cytotoxicity (Henkart, P. and Martz, E., eds.) pp. 203–218, Plenum, New York (1985).
Wright, S. C. and Bonavida, B. J. Immunol. 130, 2960–2964 (1983).
Wright, S. C., Wilber, S. M., and Bonavida, B., in Mechanisms of Cell-Mediated Cytotoxicity vol. II (Henkart, P. and Martz, E., eds.) pp. 179–200, Plenum, New York (1985).
Reynolds, C. W., Reichardt, D., Henkart, M., Millard, P., and Henkart, P. J. Exp. Med. (submitted).
Deem, R. L. and Targan, S. R. J. Immunol. 133, 72–77 (1984).
Hiserodt, J. C., Britvan, L. J., and Targan, S. R. J. Immunol. 131, 2710–2713 (1983).
Granger, G. A., Shacks, S. J., Williams, T. W., and Kolb, W. P. Nature 221, 1155–1161 (1969).
Mathews, N. and Watkins, J. F. B. J. Cancer 38, 302–309 (1978).
Carswell, E. A., Old, L. J., Kassel, R. L., Green, S., Fiore, N., and Williamson, B. Proc. Natl. Acad. Sci. USA 72, 3666–3670 (1975).
Aggarwal, B. B., Kohr. Hass, P. E., Moffat, B., Spencer, S. A., Henzel, W. J., Bringman, T. S., Hedwin, G. E., Goeddel, D. V., andHarkins, R. N. J. Biol. Chem. 260, 2345–2354 (1985).
Sayers, T. J., Ransom, J. R., Denn, A. C., III, Herberman, R. B., and Ortaldo, J. R. J. Immunol. 137, 385–390 (1986).
Ransom, J. H., Evans, C. H., McCabe, R. P., Pomato, N., Heinbaugh, J. A., Chin, M., and Hanna, M. G., Jr. Cancer Res. 45, 851–862 (1985).
Forbes, J. T. and Oeltman, T. N., in NK Cells and Other Natural Effector Cells (Herberman, R. B., ed.) pp. 977–982, Academic, New York (1982).
Ortaldo, J. R., Timonen, T. T., and Herberman, R. B. Clin. Immunol. Immunopathol. 31(3), 439–444 (1984).
Stutman, O., Dien, P., Wisum, E., and Lattime, E. C. Proc. Natl. Acad. Sci. USA 77, 2895–2898 (1980).
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1988 The Humana Press Inc.
About this chapter
Cite this chapter
Ortaldo, J.R. (1988). Human Natural Killer Cytotoxic Factor (NKCF). In: Ransom, J.H., Ortaldo, J.R. (eds) Leukolysins and Cancer. Contemporary Biomedicine, vol 8. Humana Press. https://doi.org/10.1007/978-1-4612-4586-5_8
Download citation
DOI: https://doi.org/10.1007/978-1-4612-4586-5_8
Publisher Name: Humana Press
Print ISBN: 978-1-4612-8938-8
Online ISBN: 978-1-4612-4586-5
eBook Packages: Springer Book Archive