Its Purification, Biological Properties, and Mechanism of Action
  • Craig W. Reynolds
  • Pierre Henkart
Part of the Contemporary Biomedicine book series (CB, volume 8)


The mechanism of lymphocyte cytotoxicity has been the subject of investigation for well over ten years. In spite of this intensive effort, the exact mechanism(s) involved in the destruction of target cells by lymphocytes remains controversial. Over the past few years, however, several groups have been able to demonstrate a number of common features for the cytolytic process by different lymphocyte populations, including T-cells and natural killer (NK) cells. These common features have led to the suggestion that lymphocyte-mediated cytotoxicity proceeds in a series of stages, ultimately resulting in the secretion of cytolytic molecules leading to target cell lysis. These distinct stages include: (1) the binding of effector and target cell via a specific receptor-antigen interaction, (2) a calcium- and energy-requiring rearrangement of cytoplasm and secretion of cytotoxic material into the intercellular space, (3) the interaction of the cytolytic material with target-cell membranes, leading to (4) lysis of the target cell.


Natural Killer Natural Killer Cell Target Cell Lysosomal Enzyme Cytolytic Activity 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Henkart, M. P. and Henkart, P. A., in Mechanisms in Cell Mediated Cytotoxicity (Clark, W. R. and Goldstein, P., eds.) Plenum, New York (1982).Google Scholar
  2. 2.
    Carpen, O., Virtanen, I., and Saksela, E. J. Immunol. 128, 2691–2697 (1982).PubMedGoogle Scholar
  3. 3.
    Haliotis, T., Roder, J., Klein, M., Ortaldo, J. R., Fauci, A. S., and Herberman, R. B. J. Exp. Med. 151, 1039–1048 (1980).PubMedCrossRefGoogle Scholar
  4. 4.
    Katz, P., Zaytoun, A. M., and Facui, A. S. J. Clin. Invest. 69, 1231–1238 (1982).PubMedCrossRefGoogle Scholar
  5. 5.
    Abo, T., Cooper, M. D., and Balch, C. M. J. Clin. Invest. 70, 193–197 (1982).PubMedCrossRefGoogle Scholar
  6. 6.
    Roder, J. C. and Duwe, A. K. Nature 278, 451–453 (1979).PubMedCrossRefGoogle Scholar
  7. 7.
    Itoh, K., Suzuki, R., Umezu, Y., Hanaumi, K., and Kumagai, K. J. Immunol. 129, 395–400 (1982).PubMedGoogle Scholar
  8. 8.
    Neighbour, P. A. and Huberman, H. S. J. Immunol. 128, 1236–1240 (1982).PubMedGoogle Scholar
  9. 9.
    Neighbour, P. A., Huberman, H. S., and Kress, Y. Eur. J. Immunol. 12, 588–595 (1982).PubMedCrossRefGoogle Scholar
  10. 10.
    Hoffman, T., Hirata, F., Bougnoux, P., Fraser, B. A., Goldfarb, R. H., Herberman, R. B., and Axelrod, J. Proc. Natl. Acad. Sci. USA 78, 3839–3843 (1981).PubMedCrossRefGoogle Scholar
  11. 11.
    Hattori, T., Hirata, F., Hoffman, T., Hizuta, A., and Herberman, R. B. J. Immunol. 131, 662–665 (1983).PubMedGoogle Scholar
  12. 12.
    Verhoef, J. and Sharma, S. D. J. Immunol. 131, 125–131 (1983).PubMedGoogle Scholar
  13. 13.
    Reynolds, C. W., Timonen, T., and Herberman, R. B. J. Immunol. 127, 282–287 (1981).PubMedGoogle Scholar
  14. 14.
    Reynolds, C. W., Timonen, T. T., Holden, H. T., Hansen, C. T., and Herberman, R. B. Eur. J. Immunol. 12, 577–582 (1982).PubMedCrossRefGoogle Scholar
  15. 15.
    Luini, W., Boraschi, D., Alberti, S., Aleotti, A., and Tagliabue, A. Immunology 43, 663–668 (1981).PubMedGoogle Scholar
  16. 16.
    Kumagai, K., Itoh, K., Suzuki, R., Hinuma, S., and Saitoh, F. J. Immunol. 129, 388–394 (1982).PubMedGoogle Scholar
  17. 17.
    Timonen, T., Ortaldo, J. R., and Herberman, R. B. J. Exp. Med. 153, 569–582 (1981).PubMedCrossRefGoogle Scholar
  18. 18.
    Millard, P., Henkart, M. P., Reynolds, C. W., and Henkart, P. A. J. Immunol. 132, 3197–3204 (1984).PubMedGoogle Scholar
  19. 19.
    Henkart, P. A., Millard, P. J., Reynolds, C. W., and Henkart, M. P. J. Exp. Med. 160, 75–93 (1984).CrossRefGoogle Scholar
  20. 20.
    Ward, J. M. and Reynolds, C. W. Am. J. Pathol. 111, 1–10 (1983).PubMedGoogle Scholar
  21. 21.
    Reynolds, C. W., Bere, E. W., and Ward, J. M. J. Immunol. 132, 534–540 (1984).PubMedGoogle Scholar
  22. 22.
    Podack, E. R. and Konigsberg, P. I. J. Exp. Med. 160, 695–710 (1984).PubMedCrossRefGoogle Scholar
  23. 23.
    Criado, M., Lindstrom, J. M., Anderson, C. G., and Dennert, G. J. Immunol. 135, 4245–4251 (1985).PubMedGoogle Scholar
  24. 24.
    Roder, J. C, Argov, S., Klein, M., Peterson, C, Kiessling, R., Anderson, K., and Hanson, M. Immunoogy 40, 107–116 (1980).Google Scholar
  25. 25.
    Zucker-Franklin, D., Grusky, G., and Yang, J-S. Proc. Natl. Acad. Sci. USA 80, 6977–6981 (1983).PubMedCrossRefGoogle Scholar
  26. 26.
    Zagury, D., in Mechanisms of Cell-Mediated Cytotoxicity (Clark, W. R. and Goldstein, P., eds.) Plenum, New York (1982).Google Scholar
  27. 27.
    Butterworth, A. E., Vadas, M. A., Wassom, D. L., Dessein, A., Hogan, M., Sherry, B., Gleich, G. J., and David. J. R. J. Exp. Med. 150, 1456–1471 (1979).PubMedCrossRefGoogle Scholar
  28. 28.
    Schwartz, L. B., Austen, K. F., and Wasserman, S. I. J. Immunol. 123, 1445–1450 (1979).PubMedGoogle Scholar
  29. 29.
    Mayer, M. M. Adv. Exp. Med. Biol. 146, 193–216 (1982).PubMedGoogle Scholar
  30. 30.
    Callewaert, D. M. and Makle, N. H., in Mechanisms of Cytotoxicity by NK Cells (Herberman, R. B. and Callewaert, D. M., eds.) Academic, New York (1985).Google Scholar
  31. 31.
    Reynolds, C. W., Reichardt, D., Henkart, M., Millard, P., and Henkart, P. (submitted for publication).Google Scholar
  32. 32.
    Yu, C. C, Reynolds, C. W., and Henkart, P. A. (submitted for publication).Google Scholar
  33. 33.
    Simone, C. B. and Henkart, P. J. Immunol. 124, 954–963 (1980).PubMedGoogle Scholar
  34. 34.
    Dourmashkin, R. R., Deteix, P., Simone, C. B., and Henkart, P. A. Clin. Exp. Immunol. 43, 554–650 (1980).Google Scholar
  35. 35.
    Podack, E. R. and Dennert, G. Nature (Lond.) 302, 442–445 (1983).PubMedCrossRefGoogle Scholar
  36. 36.
    Henkart, M. P. and Henkart, P. A. Adv. Exp. Med. Biol. 146, 227–242 (1982).PubMedGoogle Scholar
  37. 37.
    Blumenthal, R., Millard, P. J., Henkart, M. P., Reynolds, C. W., and Henkart, P. A. Proc. Natl. Acad. Sci. USA 81, 5551–5555 (1984).PubMedCrossRefGoogle Scholar

Copyright information

© The Humana Press Inc. 1988

Authors and Affiliations

  • Craig W. Reynolds
  • Pierre Henkart

There are no affiliations available

Personalised recommendations