Skip to main content
SpringerLink
Log in

Cell Cultures as a Model for Alcohol Research

  • Chapter
Molecular Mechanisms of Alcohol

Part of the book series: Experimental Biology and Medicine ((EBAM,volume 21))

Abstract

Mammalian cells have been maintained and propagated in vitro for more than 50 years. Cell tissue cultures also have also been an important tool in neurobiological research for many years. However, the application of cell cultures for evaluating the effects of ethanol on the nervous system is a much more recent advancement. The number of publications addressing this approach do not exceed 50, but the field is growing rapidly. The rationale for the use of cultured cells for analyzing the harmful effect of ethanol on the nervous system rests on the fact that actions produced by ethanol are ultimately exerted at a cellular level. It is a logical postulate that nerve cell cultures allow a more precise delineation of the mechanisms of alcohol effects than can be accomplished by studies in animals. The variety of cells that constitute the central nervous system can be studied individually, thereby enabling a more accurate prediction of ethanol effects than can be achieved on brain slices or animal experiments. Of special importance is the freedom from organismic influences produced during ethanol oxidation in the liver. Also hormonal and nutritional influences induced by systemic alcohol exposure can be excluded or studied separately.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 169.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 219.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Alling, C., Gustavsson, L., Hansson, E. and Rönnbäck, L (1986) Drug Alc. Dep. 18, 115–126.

    Article  CAS  Google Scholar 

  2. Anano, T., Richelson, E. and Nirenberg, M. (1972) Natl. Acad. Sci. USA, 69, 258–263.

    Article  Google Scholar 

  3. Bourre, J.M., Pascal, G., Furand, G., Masson, M., Dumont, O. and Piciotti, M. (1984) J. Neurochem. 43, 342–348.

    Article  PubMed  CAS  Google Scholar 

  4. Carbonetto, S., Gruver, M.M. and Turner, D.C. (1983) J. Neurosci. 3, 2324–2335.

    PubMed  CAS  Google Scholar 

  5. Charness, M.E., Gordon, A.S. and Diamond, I. (1983) Science 222, 1246–1248.

    Article  PubMed  CAS  Google Scholar 

  6. Chamess, M.E., Querimit, L.A. and Diamond, I. (1986) J. Biol. Chem. 261, 3164–3169.

    Google Scholar 

  7. Chamess, M.E., Wuerimit, L.A. and Henteleff, M. (1988) Biochem. Biophys. Res. Comm. 155, 138–143.

    Article  Google Scholar 

  8. Clos, J., Favre, C., Selme-Matrat, M. and Le Grand, T. (1977) Brain Res. 123, 13–16.

    Article  PubMed  CAS  Google Scholar 

  9. Cook, H.W. and Spence, M.W. (1987) Biochim. Biophys. Acta. 918, 217–229.

    PubMed  CAS  Google Scholar 

  10. Cook, H.W. and Spence, M.W. (1987) Lipids, 22, 613–619.

    Article  PubMed  CAS  Google Scholar 

  11. Davies, D.L. and Vernadakis, A. (1984) Develop. Brain Res. 16, 27–35.

    Article  CAS  Google Scholar 

  12. Faivre-Baumon, A., Puymirat, J., Loudes, C., Barret, A. and Tixer-Vidal, A. (1984) Neurosci. Lett. 44, 83–89.

    Article  Google Scholar 

  13. Gordon, AS., Wrubel, B., Collier, K., Estrin, W. and Diamond, I. (1987) in: Alcohol and the Cell (E. Rubin, ed.). The New York Academy of Sciences, NY pp. 367–374.

    Google Scholar 

  14. Grishan, J.W. and Smith, G.J. (1984) Pharmacol. Reviews. 36, 151–171.

    Google Scholar 

  15. Gundersen, R.W. and Barett, J.N. (1984) Res. 314, 21–26.

    CAS  Google Scholar 

  16. Hamprecht, B., Glaser, T., Reiser, G., Bayer, E. and Propst, F. (1977) Methods in Enzymology 109, 316–341.

    Article  Google Scholar 

  17. Hansson, E., Rönnbäck, L., Persson, L.I., Lowenthal, A., Noppe, M., Alling, C. and Karlsson, B. (1984) Brain Res. 300, 9–18.

    Article  PubMed  CAS  Google Scholar 

  18. Hansson, E. (1984) Neurochem. Res. 9, 153–172.

    Article  PubMed  CAS  Google Scholar 

  19. Hansson, E., Simosson, P. and Alling, C. (1987) Neuropharmacol. 26, 1377–1382.

    Article  CAS  Google Scholar 

  20. Hansson, E., Simonsson, P. and Alling, C. (1989) Eur. J. Pharm. Submitted.

    Google Scholar 

  21. Higashida, H., Streaty, R.A., Klee, W. and Nirenberg, M. (1986) Proc. Natl. Acad. Sci., USA 83, 942–946.

    Article  PubMed  CAS  Google Scholar 

  22. Kahn, D.J., Mitrius, J.C. and U’Pichard, D.C. (1981) Molecul. Pharmacol. 21, 17–26.

    Google Scholar 

  23. Kennedy, L.A. and Mukerji, S. (1986) Neurobehav. Toxicol. Teratol. 8, 11–15.

    PubMed  CAS  Google Scholar 

  24. Kim, H-S. (1987) Chung-Ang J. Med. 12, 551–563.

    CAS  Google Scholar 

  25. Kuriyama, K., Ohkuma, S., Tomono, S. and Hirouchi, M. (1987) Alcohol and Alcoholism, suppl. 1, 685–689.

    CAS  Google Scholar 

  26. Lacal, J.C. de la Pena, P., Moscat, J., Garcia-Barreno, P., Anderson, P.S. and Aaronson, S.A. (1987) Science 238, 533–536.

    Article  PubMed  CAS  Google Scholar 

  27. Laerum, O.D., Steinsvåg, S. and Bjerkvig, R. (1985) Acta. Neurol. Scand., 72, 529–549.

    Article  PubMed  CAS  Google Scholar 

  28. Law, P-Y., Koehler, J.E. and Loh, H.H. (1981) Molecular Pharmacol. 21, 483–491.

    Google Scholar 

  29. Law, P-Y., Horn, D.S. and Loh, H.H. (1983) Biochem. Pharmacol. 259, 4096–4104.

    Google Scholar 

  30. Ledig, M., Pillement, P., Devilliers, G. and Mandel, P. (1984) Substance Alcohol Acitons/Misuse 5, 159–168.

    CAS  Google Scholar 

  31. MacDermot, J., Higashida, H., Wilson, S.P., Matsuzawa, H., Minna, J. and Nirenberg, M. (1979) Proc. NatlAcad. Sci., USA 76, 1135–1139.

    Article  CAS  Google Scholar 

  32. Mochley-Rosen, D., Chang, F-H., Cheever, L., Kim, M., Diamond, I. and Gordon, A.S. (1988) Nature 333, 48–50.

    Google Scholar 

  33. Morrison, M., Wilce, P.A. and Shanley, B.C. (1984) Biochem. Biophys. Res. Comm. 122, 516–521.

    Article  Google Scholar 

  34. Muller, H.W., Beckh, S. and Seifert, W. (1984) Natl. Acad. Sci. USA 81, 1248–1252.

    Article  CAS  Google Scholar 

  35. Murphy, M.G., Moak, C.M. and Rao, B.G. (1987) Biochem. Pharmacol. 36, 4079–4084.

    Article  PubMed  CAS  Google Scholar 

  36. Riopelle, R.J., Dow, K.E. and Eisenhauer, E. (1984) Can. J. Physiol. Pharmacol. 62, 1032–1036.

    Article  PubMed  CAS  Google Scholar 

  37. Ritchie, T., Kim, H-S., Cole, R., deVellis, J. and Noble, E.P. (1988) Alcohol. 5, 183–187.

    Article  PubMed  CAS  Google Scholar 

  38. Robert, J., Rebel, G. and Mandel, P. (1977) Biochimie. 59, 417–423.

    Article  PubMed  CAS  Google Scholar 

  39. Robert, J., Rebel, G. and Mandel, P. (1978) J. Neurochem. 30, 543–548.

    Article  PubMed  CAS  Google Scholar 

  40. Rönnbäck, L., Hansson, E. and Alling, C. (1988) Alcohol and Alcoholism 23, 465–475.

    PubMed  Google Scholar 

  41. Simonsson, P., Hansson, E. and Alling, C. (1989a) Biochem. Pharm. Accepted.

    Google Scholar 

  42. Simonsson, P., Sun, G., Vecsei, L. and Alling, C. (1989b) Ethanol effects on bradykinin-stimulated phosphoinositide hydrolysis in NG 108–15 neuroblastoma-glioma cells. (In press)

    Google Scholar 

  43. Söderbäck, M., Hansson, E. Tottmar, O. and Rönnbäck, L. (1989) Cell Molec. Biol. (In press)

    Google Scholar 

  44. Sun, G.Y., Aradottir, S., Gustavsson, L. and Alling, C. (1989) J. Neurochem. R. (submitted)

    Google Scholar 

  45. Tabakoff, B., Nobel, E.G. and Warren, K.R. (1979) in: Nutrition and the Brain (R.J. Wurtman and J.J. Wurtman, eds). Raven Press, New York, Vol 4 pp. 159–213.

    Google Scholar 

  46. Volpe, J.J., lomori, Y., Haven, G.G. and Goldber, R.R. (1986) J. Neurochem. 46, 475–482.

    Article  PubMed  CAS  Google Scholar 

  47. Wickramasinghe, S.N. (1987) Alcoholism: Clin. Exp. Res. 11, 234–237.

    Article  CAS  Google Scholar 

  48. Yano, K., Higashida, H., Inoue, R. and Nozawa, Y. (1984) J. Biol. Chem. 259, 10201–10207.

    PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Psychiatry and Neurochemistry, Lund University, Sweden

    Christer Alling, Elisabeth Hansson & Per Simonsson

  2. Institute of Neurobiology, University of Göteborg, Sweden

    Elisabeth Hansson & Per Simonsson

Authors
  1. Christer Alling
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Elisabeth Hansson
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Per Simonsson
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Sinclair Research Farm, University of Missouri, Columbia, MO, USA

    Grace Y. Sun  & Albert Y. Sun  & 

  2. Department of Anatomy and Neurobiology, University of Missouri, Columbia, MO, USA

    P. Kevin Rudeen

  3. VA Medical Center and Department of Pharmacology, University of Minnesota School of Medicine, Minneapolis, MN, USA

    W. Gibson Wood

  4. Department of Biochemistry, National Yang-Ming Medical College, Taipei, Taiwan, ROC

    Yau-Huei Wei

Rights and permissions

Reprints and permissions

Copyright information

© 1989 The Humana Press Inc.

About this chapter

Cite this chapter

Alling, C., Hansson, E., Simonsson, P. (1989). Cell Cultures as a Model for Alcohol Research. In: Sun, G.Y., Rudeen, P.K., Wood, W.G., Wei, YH., Sun, A.Y. (eds) Molecular Mechanisms of Alcohol. Experimental Biology and Medicine, vol 21. Humana Press. https://doi.org/10.1007/978-1-4612-4514-8_14

Download citation

  • DOI: https://doi.org/10.1007/978-1-4612-4514-8_14

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-4612-8855-8

  • Online ISBN: 978-1-4612-4514-8

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation