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Isolation, Purification and Characterization of a Diuretic Peptide (AP-I) from the House Cricket, Acheta Domesticus

  • Geoffrey M. Coast
  • Colin H. Wheeler
  • Nicholas F. Totty
  • Robin J. Philp
  • Graham J. Goldsworthy
Part of the Experimental and Clinical Neuroscience book series (ECN)

Abstract

Insect diuretic peptides differ chemically and, even within a single species, several forms of diuretic peptide may be present (Wheeler and Coast, 1989). Thus, in Locusta migratoria, diuretic peptides have been described as AVP-like, ACTH-like, and as novel peptides. Only two insect diuretic peptides have been fully characterised, one from Locusta (Proux et al., 1987), and the other from Manduca sexta (Kataoka et al., 1989). They have no structural analogy to each other, but each has similarities with some vertebrate peptides. Fractionation of aqueous extracts of the corpora cardiaca of Acheta domesticus yields a number of peptides which stimulate fluid secretion by isolated Malpighian tubules (Coast and Wheeler, 1989). One peptide, Acheta peptide-I (AP-I), is associated with a major UV absorbing peak, and was selected for sequence analysis.

Keywords

Synthetic Peptide Malpighian Tubule Diuretic Activity Fast Atom Bombardment Mass Spectrometry Prolidase Activity 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Coast G.M. and Wheeler C.H. (1989) The distribution and relative potency of diuretic peptides in the house cricket, Acheta domesticus. Physiol. Entomol., In press.Google Scholar
  2. Kataoka H., Troetschler R.G., Li J.P., Kramer S.J., Carney R.L. and Schooley D.A. (1989) Isolation and identification of a diuretic peptide from the tobacco hornworm, Manduca sexta. Proc. Nat. Acad. Sci. USA, 86, 2976–2980.PubMedCrossRefGoogle Scholar
  3. Proux J. P., Miller C.A., Li J.P., Carney R.L., Girardie A., Delaage M. and Schooley D.A. (1987) Identification of an arginine vasopressin-like diuretic hormone from Locusta migratoria. Biochem. Biophys. Res. Commun., 149, 180–186.PubMedCrossRefGoogle Scholar
  4. Tarr, G.E. (1986) Manual Edman sequencing system. In Methods of protein microcharacterization (Ed. By Shively, J.E. ), pp. 162–163, Humana press, New York.Google Scholar
  5. Wheeler C.H. and Coast G.M. (1989) Assay and characterisation of diuretic factors in insects. J. Insect Physiol., In press.Google Scholar

Copyright information

© The Humana Press Inc. 1990

Authors and Affiliations

  • Geoffrey M. Coast
    • 1
  • Colin H. Wheeler
    • 1
  • Nicholas F. Totty
    • 2
  • Robin J. Philp
    • 2
  • Graham J. Goldsworthy
    • 1
  1. 1.Birkbeck CollegeLondonUK
  2. 2.Ludwig Institute for Cancer ResearchLondonUK

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