Isolation, Purification and Characterization of a Diuretic Peptide (AP-I) from the House Cricket, Acheta Domesticus
Insect diuretic peptides differ chemically and, even within a single species, several forms of diuretic peptide may be present (Wheeler and Coast, 1989). Thus, in Locusta migratoria, diuretic peptides have been described as AVP-like, ACTH-like, and as novel peptides. Only two insect diuretic peptides have been fully characterised, one from Locusta (Proux et al., 1987), and the other from Manduca sexta (Kataoka et al., 1989). They have no structural analogy to each other, but each has similarities with some vertebrate peptides. Fractionation of aqueous extracts of the corpora cardiaca of Acheta domesticus yields a number of peptides which stimulate fluid secretion by isolated Malpighian tubules (Coast and Wheeler, 1989). One peptide, Acheta peptide-I (AP-I), is associated with a major UV absorbing peak, and was selected for sequence analysis.
KeywordsHPLC Tyrosine Citrate Acetonitrile Amidated
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