Characterization of the EBV Alkaline Exonuclease Expressed in a Recombinant Prokaryotic System

  • E. Littler
  • S. A. Baylis
  • D. J. Purifoy
Chapter
Part of the Experimental Biology and Medicine book series (EBAM, volume 20)

Abstract

Epstein-Barr virus (EBV) is a human herpesvirus which is the cause of infectious mononucleosis and is associated with two malignancies, Burkitt’s lymphoma and nasopharyngeal carcinoma (NPC). One of the major constraints on studying EBV is the lack of a fully permissive tissue culture system, which has limited both the quality and quantity of EBV proteins available for analysis. However, despite this limitation the EBV genome has been shown to encode a number of open reading frames (orfs), the protein products of which are likely to be involved in DNA replication and metabolism. These proteins include a DNA polymerase; a major DNA binding protein; a ribonucleotide reductase; a thymidine kinase; a dUTPase and an alkaline DNase (1,6,9,10).

Keywords

Zinc Magnesium Lymphoma Manganese Radium 

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Copyright information

© The Humana Press Inc. 1989

Authors and Affiliations

  • E. Littler
    • 1
  • S. A. Baylis
    • 1
  • D. J. Purifoy
    • 2
  1. 1.Dept. of Molecular Biology, Paterson Institute For Cancer ResearchChristie Hospital and Holt Radium InstituteManchesterUK
  2. 2.Dept. of Molecular SciencesWelcome Research LaboratoriesKentUK

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