Characterization of the EBV Alkaline Exonuclease Expressed in a Recombinant Prokaryotic System

  • E. Littler
  • S. A. Baylis
  • D. J. Purifoy
Part of the Experimental Biology and Medicine book series (EBAM, volume 20)


Epstein-Barr virus (EBV) is a human herpesvirus which is the cause of infectious mononucleosis and is associated with two malignancies, Burkitt’s lymphoma and nasopharyngeal carcinoma (NPC). One of the major constraints on studying EBV is the lack of a fully permissive tissue culture system, which has limited both the quality and quantity of EBV proteins available for analysis. However, despite this limitation the EBV genome has been shown to encode a number of open reading frames (orfs), the protein products of which are likely to be involved in DNA replication and metabolism. These proteins include a DNA polymerase; a major DNA binding protein; a ribonucleotide reductase; a thymidine kinase; a dUTPase and an alkaline DNase (1,6,9,10).


Thymidine Kinase Ribonucleotide Reductase Infectious Mononucleosis DNase Activity Parental Plasmid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Baer et al (1984). Nature 310, 207–211.PubMedCrossRefGoogle Scholar
  2. 2.
    Chen et al (1985). J. Med. Virol. 17, 47–49.PubMedCrossRefGoogle Scholar
  3. 3.
    Chen et al (1987). J. Med. Virol. 23, 11–21.PubMedCrossRefGoogle Scholar
  4. 4.
    Clough (1979). Biochemistry 18, 4517–4521.PubMedCrossRefGoogle Scholar
  5. 5.
    Clough (1980). Proc. Natl. Acad. Sci. 77, 6194–6198.Google Scholar
  6. 6.
    Littler et al (1986). EMBO J. 5, 1959–1969.PubMedGoogle Scholar
  7. 7.
    Littler et al (1988). J. Gen. Virol. 69, 2021–2031.PubMedCrossRefGoogle Scholar
  8. 8.
    Liu et al (1984). Chinese J. of Microbiol. Immunol. 17, 21–130.Google Scholar
  9. 9.
    McGeoch et al (1986). Nuc. Acid. Res. 14, 3435–3447.CrossRefGoogle Scholar
  10. 10.
    Quinn and McGeoch (1985). Nuc. Acid Res. 13, 8143–8163.CrossRefGoogle Scholar
  11. 11.
    Tan et al (1982). J. Virol. 44, 893–899.PubMedGoogle Scholar

Copyright information

© The Humana Press Inc. 1989

Authors and Affiliations

  • E. Littler
    • 1
  • S. A. Baylis
    • 1
  • D. J. Purifoy
    • 2
  1. 1.Dept. of Molecular Biology, Paterson Institute For Cancer ResearchChristie Hospital and Holt Radium InstituteManchesterUK
  2. 2.Dept. of Molecular SciencesWelcome Research LaboratoriesKentUK

Personalised recommendations