Summary
Binding of the EBNA-1 to ori-P was studied by affinity column chromatography followed by immunoblotting with anti EBNA-1 serum. Treatment of Raj i cells with phorbol ester, which activates the EBV replicative life cycle, induced the production of two cellular competitors which upon competition uncoupled the binding of EBNA-1 to ori-P. Gel retardation assay, DNase I footprinting and methylation interference analysis showed that the two cellular competitors bound specifically to the 20x 30 bp tandem repeat clusters in the EBV ori-P.
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© 1989 The Humana Press Inc.
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Wen, L.T., Tanaka, A., Nonoyama, M. (1989). Anti EBNA-1 Protein Induced with TPA. In: Ablashi, D.V., Faggioni, A., Krueger, G.R.F., Pagano, J.S., Pearson, G.R. (eds) Epstein-Barr Virus and Human Disease • 1988. Experimental Biology and Medicine, vol 20. Humana Press. https://doi.org/10.1007/978-1-4612-4508-7_15
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DOI: https://doi.org/10.1007/978-1-4612-4508-7_15
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