Advertisement

Application of DNA Sequence and Clone Data

  • David W. Burden
  • Donald B. Whitney

Overview

The sequence of nucleotides within a DNA molecule can yield a wealth of information. Important biological features such as amino acid sequence, exact amino acid composition, and gene structure are contained within sequences. The data can be used for highly specific manipulations of cloned DNA, such as changing a single nucleotide, substituting promoters between genes, and fusing of genes to yield hybrid (heterologous) proteins. Probes can also be designed for a variety of uses, including the detection of genetic diseases and pathogenic microbes.

The nucleotide sequence of a clone is fundamental. From this data, all known restriction sites, subsequences, and coding regions can be determined. The comparison of sequences allows for the elucidation of important biological motifs, such as the Pribnow box, the alteration of sequences through site-directed mutagenesis, and gene fusion methodologies. The synthesis of homologous oligonucleotides allows for the design of highly specific probes (or primers). These oligonucleotides, or primers, have led to the development of an extremely powerful technique, the polymerase chain reaction (PCR). Based on the specificity of primers, PCR allows for the in vitro replication of small regions of DNA.

The objective of the preceding twelve chapters was to gather data from a protein and clone its associated gene. Whether you were successful in this endeavor or simply followed the process, that objective has been achieved. In reality, the means by which you cloned the MEL1 gene, however, is not important. It is the gene itself, including its nucleotide sequence, that is important.

Now that you have a gene and its sequence, what do you do with it? This chapter will focus on the analysis of DNA sequence data and on one method for its application.

Keywords

Restriction Endonuclease Recognition Site Clone Data Promoter Consensus Sequence Histidine Biosynthetic Enzyme 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Further Readings

  1. Erlich H, ed. (1989): PCR Technology. New York: Stockton PressGoogle Scholar
  2. Innis M, Gelfand D, Sninsky J, White T, eds. (1990): PCR Protocols. San Diego: Academic PressGoogle Scholar
  3. Reznikoff W, Gold L, eds. (1986): Maximizing Gene Expression. Boston: ButterworthGoogle Scholar

References

  1. Sumner-Smith M, Bozzato M, Skipper N, Davies R, Hopper J (1985): Analysis of the inducible MEL1 gene of Saccharomyces carlsbergensis and its secreted product, oc-galactosidase (melibiase). Gene 36:333–340PubMedCrossRefGoogle Scholar
  2. von Heijne G (1987): Sequence Analysis in Molecular Biology—Treasure Trove or Trivial Pursuit. San Diego: Academic PressGoogle Scholar

Copyright information

© Birkhäuser Boston 1995

Authors and Affiliations

  • David W. Burden
    • 1
  • Donald B. Whitney
    • 1
  1. 1.Biotechnology Training & Consulting, Inc.LebanonUSA

Personalised recommendations