Capillary Electrophoresis for Quantitative Genetic Analysis

  • Jill M. Kolesar
  • John G. Kuhn
Part of the Advanced Biomedical Technologies book series (ABT)

Abstract

Electrophoresis was introduced by Tiselius in 1937. He received the Nobel Prize for his work that demonstrated that protein solutions migrate in a direction and at a rate determined by their charge and mobility when an electrical current is applied. Slab gel electrophoresis (SGE) is an adaptation of this technique and has been used for decades as the primary tool for purification and analysis of nucleic acids and proteins. Gels are typically made of cross—linked polyacrylamide or agarose, and the separations are carried out in low electric fields. While many slab gel techniques are well established and effective in the analysis of nucleic acids, accurate quantitation and ease of automation remain obstacles.

Keywords

Migration High Performance Liquid Chromatography Urea Codon Argon 

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Copyright information

© Birkhäuser Boston 1998

Authors and Affiliations

  • Jill M. Kolesar
    • 1
  • John G. Kuhn
    • 2
  1. 1.School of PharmacyUniversity of Wisconsin-MadisonMadisonUSA
  2. 2.Department of PharmacologyThe University of Texas Health Science Center San AntonioSan AntonioUSA

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