Branched DNA (bDNA) Technology for Direct Quantification of Nucleic Acids: Research and Clinical Applications
provides direct quantification of nucleic acid molecules at physiological levels. Since bDNA assays do not require highly purified nucleic acid preparations, inhibitors of enzyme-dependent amplification techniques are of no concern.
allows a wide diversity of specimen types to be used.
can be used with reference standards to ensure accuracy for applications requiring absolute quantification, or without standards for applications requiring only assessment of relative changes in nucleic acid concentrations.
exhibits a high level of sensitivity (moderately expressed genes can be detected using as few as 50–100 cells). The level of sensitivity can be modulated by altering the number of target-specific extenders to accommodate specific applications.
is thoroughly tested for within-lot and between-lot precision to ensure reproducibility (2.2- to 3-fold changes can be discerned as statistically significant).
KeywordsHuman Immunodeficiency Virus Type Direct Quantification Nucleic Acid Molecule Cytokine mRNA Level bDNA Assay
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