Abstract
Research in angiogenesis has flourished in recent years thanks to ground-breaking discoveries in cell and molecular biology. Experimental studies have opened the field to clinical applications raising new hopes for patients with angiogenesis-dependent disorders. Several clinical trials with inhibitors of angiogenesis are now in progress while scores of academic laboratories and pharmaceutical companies are trying to identify key molecular events in the angiogenic process for targeted therapeutic intervention (Folkman, 1996).
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Auerbach R., Auerbach W., Polakowski I. (1991): Assays of angiogenesis: a review. Pharmac Ther 51:1–11.
Bagavandoss P., Wilks J.W. (1990): Specific inhibition of endothelial cell proliferation by thrombospondin. Biochem Biophys Res. Commun 170:867–872.
Ben Ezra D., Griffin B.W., Maftzir G., Aharonov O. (1993): Thrombospondin and in vivo angiogenesis induced by basic fibroblast growth factor or lipopolysaccharide. Invest Ophthalmol Sci 34:3601–3608.
Bowersox J.C., Sorgente N. (1982): Chemotaxis of aortic endothelial cells in response to fibronectin. Cancer Res 42:2547–2551.
Brooks PC., Clark R.A., Cheresh D.A. (1994): Requirement of vascular integrin avb3 for angiogenesis. Science 264:569–571.
Cozzolino F., Torcia M., Aldinucci D., Ziche M., Almerigogna F., Bani D., Stern D.M. (1990): Interleukin 1 as autocrine regulator of endothelial cell growth. Proc Natl Acad Sci USA 87:6487–6491.
Diglio C.A., Grammas P., Giacomelli F., Wiener J. (1989): Angiogenesis in rat aorta ring explant cultures. Lab Invest 60:523–531.
Elsdale T., Bard J. (1972): Collagen substrata for studies of cell behavior. J Cell Biol 54:626–637.
Folkman J. (1995): Clinical applications of research on angiogenesis. New Eng J Med 333(26): 1757–1763.
Folkman J. (1996): Fighting cancer by attacking its blood supply: What you need to know about cancer. Scientific Am 275:150–154.
Good D.J., Polverini P.J., Rastinejad F., LeBeau M.M., Lemons R.S., Frazier W.A., Bouck N.P. (1990): A tumor-suppressor dependent inhibitor of angiogenesis is immunologically and functionally indistinguishable from a fragment of thrombospondin. Proc Natl Acad Sci USA 87:6624–6628.
Hayman E.G., Pierschbacher M.D., Ruoslahti E. (1985): Detachment of cells from culture substrate with soluble fibronectin peptides. J Cell Biol 100:1948–1954.
Ingber D., Folkman J. (1988): Inhibition of angiogenesis through modulation of collagen metabolism. Lab Invest 59:44–51.
Ingber D.E., Madri J.A., Folkman J. (1986): A possible mechanism for inhibition of angiogenesis by angiostatic steroids: induction of capillary basement membrane dissolution. Endocrinology 119:1768–1774.
Iruela-Arispe M.L., Bornstein P., Sage H. (1990): Thrombospondin exerts an anti-angiogenic effect on cord formation by endothelial cells in vitro. Proc Natl Acad Sci USA 88: 5026–5030.
Jackson C.J., Jenkins K.L. (1991): Type I collagen fibrils promote rapid vascular tube formation upon contact with the apical side of cultured endothelium. Exp Cell Res 192:319–323.
Kawasaki S., Mori M., Awai M. (1989) Capillary growth of rat aortic segments cultured in collagen without serum. Acta Pathol Japonica 39(11):712–718.
Knedler A., Ham R. (1987): Optimized medium for clonal growth of human microvascular endothelial cells with minimal serum. In Vitro Cell Dev Biol 23:481–91.
Kubota Y., Kleinman H.K., Martin G.R., et al. (1988): Role of laminin and basement membrane in the morphological differentiation of human endothelial cells into capillary-like structures. J Cell Biol 107:1589–1598.
Leung D.W., Cachianes G., Kuang W.J., Goeddel D.V., Ferrara N. (1989): Vascular endothelial growth factor is a secreted angiogenic mitogen. Science 246:1306–1309.
Madri J.A., Williams S.K. (1983): Capillary endothelial cell cultures: phenotypic modulation by matrix components. J Cell Biol 97:153–165.
Montesano R., Orci L., Vassalli R (1983): In vitro rapid organization of endothelial cells into capillary-like networks is promoted by collagen matrices. J Cell Biol 97:1648–1652.
Nicosia R.F., Tchao R., Leighton J. (1982): Histotypic angiogenesis in vitro: light microscopic, ultrastructural, and radioautographic studies. In Vitro 18:538–549.
Nicosia R.F., Tchao R., Leighton J. (1983): Angiogenesis-dependent tumor spread in reinforced fibrin clot culture. Cancer Res 43:2159–2166.
Nicosia R.F., Madri, J.A. (1987): The microvascular extracellular matrix: developmental changes during angiogenesis in the aortic-ring plasma clot model. Am J Pathol 128:78–90.
Nicosia R.F., Ottinetti A. (1990a): Modulation of microvascular growth and morphogenesis by reconstituted basement membrane-like gel in three-dimensional culture of rat aorta: a comparative study of angiogenesis in matrigel, collagen, fibrin, and plasma clot. In Vitro Cell Dev Biol 26:119–128.
Nicosia R.F., Ottinetti A. (1990b): Growth of microvessels in serum-free matrix culture of rat aorta: a quantitative assay of angiogenesis in vitro. Lab Invest 63:115–122.
Nicosia R.F., Bonanno E. (1991): Inhibition of angiogenesis in vitro by arg-gly-asp-containing synthetic peptide. Am J Pathol 138:829–833.
Nicosia R.F., Belser P., Bonanno E., Diven J. (1991): Regulation of angiogenesis in vitro by collagen metabolism. In Mtro Cell Dev Biol 27A:961–966.
Nicosia R.F., Bonanno E., Villaschi S. (1992): Large-vessel endothelium switches to a microvascular phenotype during angiogenesis in collagen gel culture of rat aorta. Atherosclerosis 95:191–199.
Nicosia R.F., Bonanno E., Smith M. (1993): Fibronectin promotes the elongation of microvessels during angiogenesis in vitro. J Cell Physiol 154:654–661.
Nicosia R.F., Tuszynski G. (1994): Matrix-bound thrombospondin promotes angiogenesis in vitro. J Cell Biol 124:183–193.
Nicosia R.F., Villaschi S., Smith M. (1994a): Isolation and characterization of vasoformative endothelial cells from the rat aorta. In Mtro Cell Dev Biol 30A:394–399.
Nicosia R.F., Bonanno E., Yurchenco P. (1994b): Modulation of angiogenesis in vitro by lamininentactin complex. Dev Biol 164:197–206.
Nicosia R.F., Nicosia S.V., Smith M. (1994c): Vascular endothelial growth factor, platelet derived growth factor, and insulin-like growth factor-1 promote rat aortic angiogenesis in vitro. Am J Pathol 145:1023–1029.
Nicosia R.F., Villaschi S. (1995): Rat aortic smooth muscle cells become pericytes during angiogenesis in vitro. Lab Invest 73(5):658–666.
Nicosia R.F., Lin Y.J., Hazelton D., Quian X. (1996): Role of vascular endothelial growth factor in the rat aorta model of angiogenesis. J Vise Res (Abstracts IX International Vascular Biology Meeting) 33(S1):73.
Nissanov J., Tuman R.W., Graver L.M., Fortunato J.M. (1995): Automatic vessel segmentation and quantification of the rat aortic ring assay of angiogenesis. Lab Invest 73:734–739.
Pepper M.S., Vassalli J.D., Orci L., Montesano R. (1993): Biphasic effect of transforming growth factor beta 1 on in vitro angiogenesis. Exp Cell Res 204:356–363.
Piershbacher M.D., Ruoslahti E. (1984): Cell attachment activity can be duplicated by small synthetic fragments of the molecule. Nature 309:30–33.
RayChaudhury A., D’Amore P.A. (1991): Endothelial cell regulation by transforming growth factor beta. J Cell Biochem 47:224–229.
Sato N., Tsuroka N., Yamamoto M., Nishihara T., Goto T. (1991): Identification of non-heparin binding endothelial cell growth factor from rat myofibroblasts. EXS 61:179–187.
Sato N., Beitz J.G., Kato J., Yamamoto M., Clark J.W., Calabresi P., Frackelton R., Jr. (1993): Platelet-derived growth factor indirectly stimulates angiogenesis in vitro. Am J Pathol 4:1119–1130.
Taraboletti G., Belotti D., Giavazzi R. (1992): Thrombospondin modulates basic fibroblast growth factor activities on endothelial cells. EXS 61:210–213.
Villaschi S., Nicosia R.F. (1993): Angiogenic role of basic fibroblast growth factor released by rat aorta after injury. Am J Pathol 143:182–190.
Villaschi S., Nicosia R.F., Smith M. (1994a): Isolation of a morphologically and functionally distinct muscle cell type from the intimal aspect of the normal rat aorta. Evidence for smooth muscle cell heterogeneity. In Mtro Cell Dev Biol 30A:589–595.
Villaschi S., Nicosia R.F. (1994b): Paracrine interactions between fibroblasts and endothelial cells in a serum-free co-culture model: modulation of angiogenesis and collagen gel contraction. Lab Invest 71:291–299.
Vukicevic S., Kleinman H.K., Luyten F.P., Roberts A.B., Roche N.S., Reddi A.H. (1992): Identification of multiple active growth factors in basement membrane Matrigel suggests caution in interpretation of cellular activity related to extracellular matrix components. Exp Cell Res 202:1–8.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1996 Birkhäuser
About this chapter
Cite this chapter
Nicosia, R.F. (1996). The Rat Aorta Model of Angiogenesis and Its Applications. In: Little, C.D., Mironov, V., Sage, E.H. (eds) Vascular Morphogenesis: In Vivo, In Vitro, In Mente. Cardiovascular Molecular Morphogenesis. Birkhäuser Boston. https://doi.org/10.1007/978-1-4612-4156-0_9
Download citation
DOI: https://doi.org/10.1007/978-1-4612-4156-0_9
Publisher Name: Birkhäuser Boston
Print ISBN: 978-1-4612-8678-3
Online ISBN: 978-1-4612-4156-0
eBook Packages: Springer Book Archive