Immunocytochemical Localization of Ionotropic Glutamate Receptors (GluRs) in Neural Circuits

  • Ronald S. Petralia
Part of the The Receptors book series (REC)


The overall distribution of ionotropic and metabotropic GluRs in the nervous system has been determined through numerous studies using ligand binding, in situ hybridization, and immunocytochemistry, as has been described in several recent reviews (Nakanishi, 1992, 1994; Seeburg, 1993; Henley, 1994; Hollmann and Heinemann, 1994; Conn and Patel, 1994; Petralia and Wenthold, 1995). In addition, several chapters in this book describe the ionotropic GluR distribution, determined through binding studies and/or in situ hybridization (see Chapters 7, 8, and 14). This chapter concentrates on the functional role that GluRs, particularly the ionotropic types, play in neural circuitry, and stresses the evidence provided by immunocytochemical studies at both light and electron microscopic levels. It does not include comprehensive descriptions of distributions of ionotropic GluR antibody immunostaining, since this has been covered in major papers (α-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid [AMPA]—Petralia and Wenthold, 1992; Martin et al., 1993b; Tachibana et al., 1994; kainate—Petralia et al., 1994a; N-methyl-D-aspartate [NMDA]—Petralia et al., 1994b,c; delta—Mayat et al., 1995).


NMDA Receptor Purkinje Cell Glutamate Receptor Metabotropic Glutamate Receptor Postsynaptic Density 
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© Humana Press Inc. 1997

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  • Ronald S. Petralia

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