Skip to main content
SpringerLink
Log in

Pharmacology of Recombinant NMDA Receptors

Possible Mechanisms for NMDA Receptor Heterogeneity

  • Chapter
The Ionotropic Glutamate Receptors

Part of the book series: The Receptors ((REC))

Abstract

Glutamate acts postsynaptically at a variety of receptors, including ionotropic and metabotropic types (Choi, 1988; Collingridge and Lester, 1989; Lipton, 1993). The ionotropic receptors are separated by their prototypic agonists into α-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA) receptors, kainate receptors, and N-methyl-D-aspartate (NMDA) receptors. The first two types are glutamate-gated cation channels that are relatively impermeable to calcium, whereas the NMDA receptor is a glutamate-gated cation channel with a high permeability to calcium.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 129.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 169.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Anand, R., Conroy, W. G., Schoepfer, R., Whiting, P., and Lindstrom, J. (1991) Neuronal nicotinic acetylcholine receptors expressed in Xenopus oocytes have a pentameric quarternary structure. J. Biol. Chem. 266, 11,192–11,198.

    Google Scholar 

  • Anantharam, V., Panchal, R. G., Wilson, A., Kolchine, V. V., Treistman, S. N., and Bayley, H. (1992) Combinatorial RNA splicing alters the surface charge on the NMDA receptor. FEBS Lett. 305, 27–30.

    Article  PubMed  CAS  Google Scholar 

  • Anegawa, N. J., Lynch, D. R., and Pritchett, D. L. (1994) Characterization of 3H dichlorokynurenic acid binding in cloned NMDA receptors expressed in transfected cells. Neurosci. Abst. 20, 469.

    Google Scholar 

  • Anegawa, N. J., Lynch, D. R., Verdoorn, T., and Pritchett, D. L. (1995) Transfection of 293 cells with NMDA receptor subunits leads to cell death. J. Neurochem. 64, 2005–2012.

    Google Scholar 

  • Beaton, J. A., Stemsrud, K., and Monaghan, D. T. (1992) Identification of a novel N-methyl-D-aspartate receptor population in the rat medial thalamus. J. Neurochem. 59, 754–757.

    Article  PubMed  CAS  Google Scholar 

  • Benveniste, M. and Mayer, M. L. (1993) Multiple effects of spermine on N-methyl-D-aspartate responses of rat cultured hippocampal neurons. J. Physiol. 464, 131–163.

    PubMed  CAS  Google Scholar 

  • Bernard, A., Rafiki, A., Ben-Ari, Y., and Khrestrchatisky, M. (1994) Molecular characterization of novel NR2C subunits of the NMDA receptor, generated by alternative splicing during rat cerebellar development. Neurosci. Abst. 20, 214.10.

    Google Scholar 

  • Buller, A. L., Larson, H. C., Schneider, B. E., Beaton, J. A., Morrisett, R.A., and Monaghan, D. T. (1994) The molecular basis of NMDA receptor subtypes: native receptor diversity is predicted by subunit composition. J. Neurosci. 14, 5471–5484.

    PubMed  CAS  Google Scholar 

  • Burnashev, N., Schoepfer, R., Monyer, H., Rupperberg, J. P., Gunther, W., Seeburg, P. H., and Sakmann, B. (1992) Control by asparagine residues of calcium permeability, and magnesium block in NMDA receptors. Science 257, 1415–1419.

    Article  PubMed  CAS  Google Scholar 

  • Chazot, P. L., Cik, M., and Stephenson, F. A. (1992) Immunological detection of the NMDAR1 glutamate receptor subunit expressed in embryonic kidney 293 cells and in rat brain. J. Neurochem. 59, 1176–1178.

    Article  PubMed  CAS  Google Scholar 

  • Chazot, P. L., Coleman, S. K., Cik, M., and Stephenson, F. A. (1994) Molecular characterization of N-methyl-D-aspartate receptors expressed in mammalian cells yields evidence for the coexistence of three subunit types within a discrete receptor molecule. J. Biol. Chem. 269, 24,403–24,409.

    Google Scholar 

  • Chen, C. and Okayama, H. (1987) High efficiency transfection of mammalian cells by plasmid DNA. Mol. Cell Biol. 7, 2745–2752.

    PubMed  CAS  Google Scholar 

  • Choi, D. (1988) Calcium mediated neurotoxicity: relationship to specific channel types and role in ischemic damage. Trends Neurosci. 7, 357–368.

    Google Scholar 

  • Cik, M., Chazot, P. L., and Stephenson, F. A. (1993) Optimal expression of cloned NMDAR1/NMDA R2A heteromeric glutamate receptors: a biochemical characterization. Biochem. J. 296, 877–883.

    PubMed  CAS  Google Scholar 

  • Cik, M., Chazot, P. L., and Stephenson, F. A. (1994) Expression of NMDAR1–1a (N598Q)/NMDAR2A receptors results in decreased cell mortality. Eur. J. Pharmacol. 266, 1–3.

    Article  Google Scholar 

  • Collingridge, G. L. and Lester, R. A. J. (1989) Excitatory amino acid receptors in the vertebrate central nervous system. Pharmacol. Rev. 41, 143–210.

    PubMed  CAS  Google Scholar 

  • Durand, G. ML, Gregor, P., Zheng, X., Bennett, M. V., Uhl, G. R., and Zukin, R. S. (1992) Cloning of an apparent splice variant of the rat N-methyl-D-aspartate receptor NMDAR1 with altered sensitivity to polyamines and activators of protein kinase C. Proc. Natl. Acad. Sci. USA 89, 9359–9363.

    Article  PubMed  CAS  Google Scholar 

  • Fagg, G. E. (1987) Phencyclidine and related drugs bind to the activated N-methyl-D-aspartate receptor-channel complex in rat brain membranes. Neurosci. Lett. 76, 221–227.

    Article  PubMed  CAS  Google Scholar 

  • Farrant, M., Feldmeyer, D., Takahashi, T., and Cull-Candy, S. G. (1993) NMDA receptor diversity in the developing cerebellum. Nature 368, 335–338.

    Article  Google Scholar 

  • Fletcher, E. J. and MacDonald, J. F. (1993) Haloperidol interacts with the strychnine-insensitive glycine site at the NMDA receptor in cultured mouse hippocampal neurones. Eur. J. Pharmacol. 235, 291–295.

    Article  PubMed  CAS  Google Scholar 

  • Frandsen, A. and Schousboe, A. (1991) Dantrolene prevents glutamate cytotoxicity and Ca++ release from intracellular stores in cultured cerebral cortical neurons. J. Neurochem. 56, 1075–1078.

    Article  PubMed  CAS  Google Scholar 

  • Franklin, P. H. and Murray, T. F. (1992) High-affinity [3H]dextrorphan binding in rat brain is localized to a noncompetitive antagonist site of the activated N-methyl-D-aspartate receptor cation channel. Mol. Pharmacol. 41, 134–146.

    PubMed  CAS  Google Scholar 

  • Gallagher, M. J., Pritchett, D. B., and Lynch, D. R. (1996) Separation of ifenprodil and polyamine effects at recombinant NMDA receptors. J. Biol. Chem. 271, 9603–9611.

    Article  PubMed  CAS  Google Scholar 

  • Grimwood, S., LeBourdelles, B., and Whiting, P. J. (1995) Recombinant human NMDA homomeric NR1 receptors expressed in mammalian cells. J. Neurochem. 64, 525–530.

    Article  PubMed  CAS  Google Scholar 

  • Hirai, H., Kirsch, J., Laube, B., Betz, H., and Kuhse, J. (1996) The glycine binding site of the N-methyl D-aspartate receptor subunit NR 1—identification of novel coagonist potentiation in the extracellular M3-M4 loop region. Proc. Natl. Acad. Sci. USA 93, 6031–6036.

    Article  PubMed  CAS  Google Scholar 

  • Hollmann, M., Boulter, J., Maron, C., Beasley, L., Sullivan, J., Pecht, G., and Heinemann, S. (1993) Zinc potentiates agonist-induced currents at certain splice variants of the NMDA receptor. Neuron 10, 943–954.

    Article  PubMed  CAS  Google Scholar 

  • Hollmann, M., Maron, C., and Heinemann, S. (1994) N-glycosylation site tagging suggests a three transmembrane domain topology for the glutamate receptor GluR1. Neuron 13, 1331–1343.

    Article  PubMed  CAS  Google Scholar 

  • Huettner, J. E. and Bean, B. P. (1988) Block of N-methyl-D-aspartate-activated current by the anticonvulsant MK 801: selective binding to open-channels. Proc. Natl. Acad. Sci. USA 85, 1307–1311.

    Article  PubMed  CAS  Google Scholar 

  • Ilvin, V., Guastella, J., Cai, S. X., Weber, E., and Woodward, R. M. (1994) Subunit specific inhibition of cloned NMDA receptors by haloperidol. Neurosci. Abst. 20, 1143.

    Google Scholar 

  • Ishii, T., Moriyoshi, K., Sugihara, H., Sakurada, K., Kadotani, H., Yokoi, M., Akazawa, C., Shigemoto, R., Mizuno, N., Masu, M., and Nakanishi S. (1993) Molecular characterization of a family of N-methyl-D-aspartate receptor subunits. J. Biol. Chem. 268, 2836–2843.

    PubMed  CAS  Google Scholar 

  • Kashiwagi, K., Fukuchi, J., Chao, J., Igarashi, K., and Williams, K. (1996) An aspartate residue in the extracellular loop of the N-methyl D-aspartate receptor controls sensitivity to spermine and protons. Mol. Pharmacol. 49, 1131–1141.

    PubMed  CAS  Google Scholar 

  • Kleckner, N. W. and Dingledine, R. (1988). Requirement for glycine in activation of NMDA receptors expressed in Xenopus oocytes. Science 241, 835–837.

    Article  PubMed  CAS  Google Scholar 

  • Kohr, G., Eckardt, S., Luddens, H., Monyer, H., and Seeburg, P.H. (1994) NMDA receptor channels: subunit-specific potentiation by reducing agents. Neuron 12, 1031–1040.

    Article  PubMed  CAS  Google Scholar 

  • Kuryatov, A., Laube, B., Betz, H., and Kuhse, J. (1994) Mutational analysis of the glycine-binding site of the NMDA receptor: structural similarity with bacterial amino acid-binding proteins. Neuron 12, 1291–1300.

    Article  PubMed  CAS  Google Scholar 

  • Laube, B., Kuryatov, A., Kuhse, J., and Betz, H. (1993) Glycine—glutamate interactions at the NMDA receptor: role of cysteine residues. FEBS Lett. 335, 331–334.

    Article  PubMed  CAS  Google Scholar 

  • Laurie, D. J. and Seeburg, P. H. (1994a) Ligand affinities at recombinant N-methyl-D-aspartate receptors depend on subunit composition. Eur. J. Pharmacol. 268, 335–345.

    Article  PubMed  CAS  Google Scholar 

  • Laurie, D. J. and Seeburg, P. H. (1994b) Regional and developmental heterogeneity in splicing of the rat brain NMDAR1 mRNA. J. Neurosci. 14, 3180–3194.

    PubMed  CAS  Google Scholar 

  • Lipton, S. (1993) Prospects for clinically tolerated NMDA antagonists: open-channel blockers and alternative redox states of nitric oxide. Trends Neurosci. 16, 527–532.

    Article  PubMed  CAS  Google Scholar 

  • Lipton, S. and Rosenberg, P. A. (1994) Excitatory amino acids as a final common pathway for neurologic disorders. N. Engl. J. Med. 330, 613–622.

    Article  PubMed  CAS  Google Scholar 

  • Lynch, D. R., Anegawa, N. J., Verdoorn, T., and Pritchett, D. B. (1994) N-methyl-D-aspartate receptors: different subunit requirement for binding of glutamate antagonists, glycine antagonists, and channel-blocking agents. Mol. Pharm. 45, 540–545.

    CAS  Google Scholar 

  • Lynch, D. R. and Gallagher, M. (1996) Interactions of haloperidol with the NMDA receptor: pharmacology and developmental regulation. J. Pharmacol. Exp. Ther. (in press).

    Google Scholar 

  • Lynch, D. R., Lawrence, J. J., Lenz, S., Anegawa, N. J., Dichter, M., and Pritchett, D. B. (1995) Pharmacological characterization of heterodimeric NMDA receptors composed of NR1a and 2B subunits: differences with receptors formed from NR1a and 2A. J. Neurochem. 64, 1462–1468.

    Article  PubMed  CAS  Google Scholar 

  • Maragos, W. F., Penney, J. B., and Young, A. B. (1988) Anatomic correlation of NMDA and [3H]TCP labeled receptors in rat brain. J. Neurosci. 8, 493–501.

    PubMed  CAS  Google Scholar 

  • Marti, T., Benke, D., Mertens, S., Heckendorn, R., Pozza, M., Allgeier, H., Angst, C., Laurie, D., Seeburg, P., and Mohler, H. (1993) Molecular distinction of three N-methyl-D-aspartate-receptor subtypes in situ and developmental receptor maturation demonstrated with the photoaffinity ligand 125I-labeled CGP 55802A. Proc. Natl. Acad. Sci. USA 90, 8434–8438.

    Article  PubMed  CAS  Google Scholar 

  • Mcdonald, J. W., Penney, J. B., Johnston, M. V., and Young, A. B. (1990) Characterization and regional distribution of strychnine-insensitive [3H]glycine binding sites in rat brain by quantitative receptor autoradiography. Neuroscience 35, 653–668.

    Article  PubMed  CAS  Google Scholar 

  • McKernan, R. M., Quirk, K., Prince, R., Cox, P. A., Gillard, N. P., Ragan, C. I., and Whiting, P. (1991) GABA-A receptor subtypes immunopurified from rat brain with alpha subunit-specific antibodies have unique pharmacological properties. Neuron 7, 667–676.

    Article  PubMed  CAS  Google Scholar 

  • Meguro, H., Mori, H., Araki, K., Kushiya, E., Kutsuwada, T., Yamazaki, M., Kumanishi, T., Arakawa, M., Sakimura, K., and Mishina, M. (1992) Functional characterization of a heteromeric NMDA receptor channel expressed from cloned cDNAs. Nature 357, 70–74.

    Article  PubMed  CAS  Google Scholar 

  • Monaghan, D. T. and Beaton, J. A. (1991) Quinolinate differentiates between forebrain and cerebellar NMDA receptors. Eur. J. Pharmacol. 194, 123–125.

    Article  PubMed  CAS  Google Scholar 

  • Monaghan, D. T., Olverman, H. J., Nguyen, L., Watkins, J. C., and Cotman, C. W. (1988) Two classes of N-methyl-D-aspartate recognition sites: differential distribution and differential regulation by glycine. Proc. Natl. Acad. Sci. 85, 9836–9840.

    Article  PubMed  CAS  Google Scholar 

  • Monyer, H., Burnashev, N., Laurie, D. J., Sakmann, B., and Seeburg, P. H. (1994) Developmental and regional expression in the rat brain and functional properties of four NMDA receptors. Neuron 12, 529–540.

    Article  PubMed  CAS  Google Scholar 

  • Monyer, H., Sprengel, R., Schoepfer, R., Herb, A., Higuchi, M., Lomeli, H., Burnashev, N., Sakmann, B., and Seeburg, P. H. (1992) Heteromeric NMDA receptors: molecular and functional distinction of subtypes. Science 256, 1217–1221.

    Article  PubMed  CAS  Google Scholar 

  • Mori, H., Masaki, H., Yamakura, T., and Mishina, M. (1992) Identification by mutagenesis of a magnesium block site of the NMDA receptor channel. Nature 358, 673–675.

    Article  PubMed  CAS  Google Scholar 

  • Moriyoshi, K., Masu, M., Ishii, T., Shigemoto, R., Mizuno, N., and Nakanishi S. (1991) Molecular cloning and characterization of the rat NMDA receptor. Nature 354, 31–36.

    Article  PubMed  CAS  Google Scholar 

  • Murphy, S. N., Thayer, S. A., and Miller, R. J. (1987) The effects of excitatory amino acids on intracellular calcium in single mouse striatal neurons in vitro. J. Neurosci. 7,4145–4158.

    PubMed  CAS  Google Scholar 

  • Paoletti, Neyton, J., and Ascher, P. (1995) Glycine independent and subunit specific potentiation of NMDA responses by extracellular Mg+2. Neuron 15, 1109–1120.

    Article  PubMed  CAS  Google Scholar 

  • Porter, R. H. and Greenamyre, J. T. (1995) Regional variations in the pharmacology of NMDA receptor channel blockers: implications for therapeutic potential. J. Neurochem. 64, 614–623.

    Article  PubMed  CAS  Google Scholar 

  • Pritchett, D. B., Sontheimer, H., Gorman, C. M., Kettenmann, H., Seeburg, P. H., and Schofield, P. R. (1989) Transient expression shows ligand gating and allosteric potentiation of GABAA receptor subunits. Science 242, 1306–1308.

    Article  Google Scholar 

  • Rajdev, S. and Reynolds, I. J. (1992) Effects of monovalent and divalent cations on 3(+)-125I-iododizolcipine binding to the N-methyl-D-aspartate receptor of rat brain membranes. J. Neurochem. 58, 1469–1476.

    Article  PubMed  CAS  Google Scholar 

  • Randall, R. D. and Thayer, S. A. (1992) Glutamate-induced calcium transient triggers delayed calcium overload and neurotoxicity in rat hippocampal neurons. J. Neurosci. 12, 1882–1895.

    PubMed  CAS  Google Scholar 

  • Ransom, R. W. and Deschenes, N. L. (1990) Polyamines regulate glycine interaction with the N-methyl-D-aspartate receptor. Synapse 5, 294–298.

    Article  PubMed  CAS  Google Scholar 

  • Reynolds, I. J. (1990) Arcaine is a competitive antagonist of the polyamine site on the NMDA receptor. Eur. J. Pharmacol. 177, 215,216.

    Google Scholar 

  • Reynolds, I. J. (1994) [3H]CGP 39653 binding to the agonist site of the N-methyl-D-aspartate receptor is modulated by Mg2+ and polyamines independently of the arcaine-sensitive polyamine site. J. Neurochem. 62, 54–62.

    Article  PubMed  CAS  Google Scholar 

  • Reynolds, I. J., and Miller, R. J. (1989) Ifenprodil is a novel type of N-methyl-D-aspartate receptor antagonist: interaction with polyamines. Mol. Pharmacol. 36, 758–765.

    PubMed  CAS  Google Scholar 

  • Reynolds, I. J., Murphy, S. N., and Miller, R. J. (1987) [3H]labeled MK-801 binding to the excitatory amino acid receptor complex from rat brain is enhanced by glycine. Proc. Natl. Acad. Sci. USA 84, 7744–7748.

    Article  PubMed  CAS  Google Scholar 

  • Reynolds, I. J. and Palmer, A. M. (1991) Regional variations in 3H-MK801 binding to rat brain N-methyl-D-aspartate receptors. J. Neurochem. 56, 1731–1740.

    Article  PubMed  CAS  Google Scholar 

  • Rock, D. M. and Macdonald, R. L. (1992) The polyamine spermine has multiple actions on N-methyl-D-aspartate receptor single-channel currents in cultured cortical neurons. Mol. Pharmacol. 41, 83–88.

    PubMed  CAS  Google Scholar 

  • Sacaan, A. I. and Johnson, K. M. (1990) Spermine enhances binding to the glycine site associated with the N-methyl-D-aspartate receptor complex. Mol. Pharmacol. 36, 836–883.

    Google Scholar 

  • Sakurada, K., Masu, M., and Nakanishi, S. (1993) Alteration of calcium permeability and sensitivity to magnesium and channel blockers by a single amino acid substitution in the N-methyl-D-aspartate receptor. J. Biol. Chem. 268, 410–415.

    PubMed  CAS  Google Scholar 

  • Sakurai, S. Y., Penney, J. B., and Young, A. B. (1993) Regionally distinct N-methyl-D-aspartate receptors distinguished by quantitative autoradiography of [3H]MK 801 in rat brain. J. Neurochem. 60, 1344–1353.

    Article  PubMed  CAS  Google Scholar 

  • Segal, M. and Manor, D. (1992) Confocal microscopic imaging of [Ca++]. in cultured rat hippocampal neurons following exposure to N-methyl-D-aspartate. J. Physiol. 448, 655–676.

    PubMed  CAS  Google Scholar 

  • Sheng, M., Cummings, J., Roidan, L. A., Jan, Y. N., and Jan, L. Y. (1994) Changing subunit composition of heteromeric NMDA receptors during development of rat cortex. Nature 368, 144–147.

    Article  PubMed  CAS  Google Scholar 

  • Sine, S. and Claudio T. (1991) Gamma and delta subunits regulate the affinity and the cooperativity of ligand-binding to the acetylcholine receptor. J. Biol. Chem. 266, 19,369–19,377.

    Google Scholar 

  • Sonders, M. S., Barmettler, P., Lee, J. A., Kitahara, Y., Keana, J. F., and Weber, E. (1990) A novel photoaffinity ligand for the phencyclidine site of the N-methyl-D-aspartate receptor labels a Mr 120,000 polypeptide. J. Biol. Chem. 265, 6776–6781.

    PubMed  CAS  Google Scholar 

  • Standaert, D., Tesla, C. M., Penney, J. B., and Young, A. B. (1993) Alternately spliced isoforms of the NMDA R1 glutamate receptor subunit: differential expression in the basal ganglia of the rat. Neurosci. Lett. 152, 161–164.

    Article  PubMed  CAS  Google Scholar 

  • Stephenson, F. A., Duggan, M. J., and Pollard, S. (1990) The gamma 2 subunit is an integral component of the gamma aminobutyric acid receptor but the alpha 1 polypeptide is the principal site of the agonist benzodiazepine photoaffinity labelling reaction. J. Biol. Chem. 265, 21,160–21,165.

    Google Scholar 

  • Subramanian, S. and McGonigle, P. (1991) Quantitative autoradiographic characterization of the binding of (+)-5-methyl-10,11-dihydro-5H-dibenzo[a,d]cyclohepten-5,1-imine ([3H]-MK 801) in rat brain: regional effects of polyamines. J. Pharmacol. Exp. Ther. 256, 814–819.

    Google Scholar 

  • Sugihara, H., Moriyoshi, K., Ishii, T., Masu, M., and Nakanishi, S. (1992) Structure and properties of 7 isoforms of the NMDA receptor generated by alternative splicing. Biochem. Biophys. Res. Commun. 185, 826–832.

    Article  PubMed  CAS  Google Scholar 

  • Sullivan, J. M., Traynelis, S. F., Chen, H. S., Escobar, W., Heinemann, S. F., and Lipton, S. A. (1994) Identification of two cysteine residues that are required for redox modulation of the NMDA subtype of glutamate receptor. Neuron 13, 929–936.

    Article  PubMed  CAS  Google Scholar 

  • Tymianski, M., Charlton, M. P., Carlen, P. L., and Tator, C. H. (1993) Source specificity of early calcium neurotoxicity in cultured embryonic spinal neurons. J. Neurosci. 13, 2085–2104.

    PubMed  CAS  Google Scholar 

  • van Amsterdam, F. T., Gilbertyi, A., Mugnaini, M., and Ratti, E. (1992) 3-(+)-2-carboxypiperazin-4-yl propyl-1-phosphonic acid recognizes two N-methyl-D-aspartate binding sites in rat cerebral cortex membranes. J. Neurochem. 59, 1850–1855.

    Article  PubMed  Google Scholar 

  • Wafford, K. A., Bain, C. J., Le Bourdelles, B., Whiting, P. J., and Kemp, J. A. (1993) Preferential co-assembly of recombinant NMDA receptors composed of three different subunits. Neuroreport. 4, 1347–1349.

    Article  PubMed  CAS  Google Scholar 

  • Wafford, K. A., Kathoria, M., Bain, C. J., Marshall, G., Le Bourdelles, B., Kemp, J. A., and Whiting, P. J. (1995) Identification of amino acids in the N-methyl-D-aspartate receptor NR1 subunit that contribute to the glycine binding site. Mol. Pharmacol. 47, 374–380.

    PubMed  CAS  Google Scholar 

  • Watanabe, M., Inoue, Y., Sakimura, K., and Mishina, M. (1992) Developmental changes in distribution of NMDA receptor channel subunit mRNAs. Neuroreport 3, 1138–1140.

    Article  PubMed  CAS  Google Scholar 

  • Williams, K. (1993) Ifenprodil discriminates subtypes of the N-methyl-D-aspartate receptor: selectivity and mechanisms at recombinant heteromeric receptors. Mol. Pharmacol. 44, 851–859.

    PubMed  CAS  Google Scholar 

  • Williams, K. C., Hanna, J., and Molinoff, P. B. (1991) Developmental changes in the sensitivity of the N-methyl-D-aspartate receptor to polyamines. Mol. Pharmacol. 40, 774–782.

    PubMed  CAS  Google Scholar 

  • Williams, K., Kashiwagi, K., Fukuchi, J., and Igarashi, K. (1995) An acidic amino acid in the N-methyl D-aspartate receptor is important for spermine stimulation. Mol. Pharmacol. 48, 1087–1098.

    PubMed  CAS  Google Scholar 

  • Williams, K. C., Russell, S. L., Shen, Y. M., and Molinoff, P. B. (1993) Developmental switch in the expression of NMDA receptors occurs in vivo and in vitro. Neuron 10, 267–278.

    Article  PubMed  CAS  Google Scholar 

  • Williams, K. C., Zappia, A. M., Pritchett, D. B., Shen, Y. M., and Molinoff, P. B. (1994) Sensitivity of the N-methyl-D-aspartate receptor to polyamines is controlled by NR2 subunits. Mol. Pharmacol. 45, 803–809.

    PubMed  CAS  Google Scholar 

  • Yoneda, Y., Suzuki, T., and Ogita, K. (1994) Differential profiles of binding of a radio-labeled agonist and antagonist at a glycine recognition domain on the N-methyl-D-aspartate receptor ionophore complex in rat brain. J. Neurochem. 62, 102–112.

    Article  PubMed  CAS  Google Scholar 

  • Yoneda, Y., Suzuki, T., Ogita, K., and Han, D. (1993) Support for radiolabeling of a glycine recognition domain on the N-methyl-D-aspartate receptor ionophore complex by 5,7-[3H]dichlorokyurenate in rat brain. J. Neurochem. 60, 634–645.

    Article  PubMed  CAS  Google Scholar 

  • Wong, E. H. F., Knight, A. R., and Woodruff, G. N. (1988) [3H]MK-801 labels a site on the N-methyl-D-aspartate receptor channel complex in rat brain membranes. J. Neurochem. 50, 274–281.

    Article  PubMed  CAS  Google Scholar 

  • Zhang, L., Zheng, X., Paupard, M. C., Wang, A. P., Santchi, L., Friedman, L. K., Zukin, R. S., and Bennett, M. V. (1994) Spermine potentiation of recombinant N-methyl-D-aspartate receptors is affected by subunit composition. Proc. Natl. Acad. Sci. USA 91, 10,883–10,887.

    Google Scholar 

  • Zheng, X., Zhang, L., Durand, G. M., Bennett, M. V., and Zukin, R. S. (1994) Mutagenesis rescues spermine and Zn2+ potentiation of recombinant NMDA receptors. Neuron 12, 811–818.

    Article  PubMed  CAS  Google Scholar 

Download references

Authors
  1. David R. Lynch
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Michael J. Gallagher
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Shelley J. Lenz
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Norifusa J. Anegawa
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Elfrida L. Grant
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Department of Pharmacology, University of Nebraska Medical Center, Omaha, NE, USA

    Daniel T. Monaghan

  2. National Institute on Deafness and Other Communication Disorders, National Institutes of Health, Bethesda, MD, USA

    Robert J. Wenthold

Rights and permissions

Reprints and permissions

Copyright information

© 1997 Humana Press Inc.

About this chapter

Cite this chapter

Lynch, D.R., Gallagher, M.J., Lenz, S.J., Anegawa, N.J., Grant, E.L. (1997). Pharmacology of Recombinant NMDA Receptors. In: Monaghan, D.T., Wenthold, R.J. (eds) The Ionotropic Glutamate Receptors. The Receptors. Humana Press. https://doi.org/10.1007/978-1-4612-3962-8_13

Download citation

  • DOI: https://doi.org/10.1007/978-1-4612-3962-8_13

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-4612-8442-0

  • Online ISBN: 978-1-4612-3962-8

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation