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Gonadotropins

Normal Physiology
  • François P. Pralong
  • William F. CrowleyJr.
Part of the Contemporary Endocrinology book series (COE, volume 3)

Abstract

The gonadotropins, luteinizing hormone (LH) and follicle stimulating hormone (FSH), are dimeric pituitary glycoprotein hormones controlling gonadal steroidogenesis and gametogenesis. Their biosynthesis and secretion is tightly regulated by counterbalancing positive inputs from the hypothalamus (GnRH) and negative feedback from the gonads (steroid and peptide hormones). The differential secretion of FSH vs LH can also be achieved by local factors produced within the pituitary gland itself (activin, in-hibin, and follistatin). Integration of these different signals by the gonadotrope results in the coordinate secretion of LH and FSH, ultimately promoting sexual maturation and normal reproductive function. Therefore, gonadotropins play a central role in reproductive biology.

Keywords

Luteinizing Hormone Follicle Stimulate Hormone Luteinizing Hormone Receptor Gonadotropin Secretion Luteinizing Hormone Surge 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Pierce JG, Parsons TE Glycoprotein hormones: structure and function. Ann Rev Biochem 1981, 50:465–495.PubMedGoogle Scholar
  2. 2.
    Gharib SD, Wierman ME, Shupnik MA, Chin WW. Molecular biology of the pituitary gonadotropins. Endocrine Rev 1990; 11:177–199.Google Scholar
  3. 3.
    Baenziger JU, Green ED. Pituitary glycoprotein hormone oligosaccharides: structure, synthesis and function of the asparagine-linked oligosaccharides on lutropin, follitropin and thyrotropin. Biochem Biophys Acta 1988; 947:287–306.PubMedGoogle Scholar
  4. 4.
    Naylor SL, Chin WW, Goodman HM, Lalley PA, Grzeschik KH, Sakaguchi AY. Chromosome assignment of genes encoding the alpha and beta subunits of glycoprotein hormones in man and mouse. Som Cell Genetics 1983; 9:757–770.Google Scholar
  5. 5.
    Fiddes JC, Goodman HM. Isolation, cloning and sequence analysis of the cDNA for the a-subunit of human chorionic gonadotropin. Nature 1979; 281:351–356.PubMedGoogle Scholar
  6. 6.
    Boorstein WR, Vamvakopoulos NC, Fiddes JC. Human chorionic gonadotropin b-subunit is encoded by at least eight genes arranged in tandem and inverted pairs. Nature 1982; 300:419–422.PubMedGoogle Scholar
  7. 7.
    Julier C, Weil D, Couillin P, et al. The beta chorionic gonadotropin-beta luteinizing gene cluster maps to human chromosome 19. Human Genetics 1984; 67:174–177.Google Scholar
  8. 8.
    Talmadge K, Boorstein WR, Fiddes JC. The human genome contains seven genes for the beta-subunit of chorionic gonadotropin but only one gene for the beta-subunit of luteinizing hormone. DNA 1983; 2:281–289.PubMedGoogle Scholar
  9. 9.
    Sawitzke AL, Griffin J, Odel WD. Purified preparations of human luteinizing hormone are contaminated with small amounts of chorionic gonadotropin-like material. J Clin Endocrinol Metab 1991; 72:841–846.PubMedGoogle Scholar
  10. 10.
    Jameson JL, Becker CB, Lindell CM, Habener JE Human follicle-stimulating hormone b-subunit gene encodes multiple messenger ribonucleic acids. Mol Endocrinol 1988; 2:806–815.PubMedGoogle Scholar
  11. 11.
    Watkins PC, Eddy R, Beck AK, et al. DNA sequence and regional assignment of the human follicle-stimulating hormone beta-subunit gene to the short arm of human chromosome 11. DNA 1987; 6:205–212.PubMedGoogle Scholar
  12. 12.
    Whitcomb RW, O’Dea LS, Finkelstein JS, Heavern DM, Crowley WF, Jr. Utility of free alpha-subunit as an alternative neuroendocrine marker of gonadotropin-releasing hormone (GnRH) stimulation of the gonadotroph in the human: evidence from normal and GnRH-deficient men. J Clin Endocrinol Metab 1990; 70:1654–1661.PubMedGoogle Scholar
  13. 13.
    Morell AG, Gregoriadis G, Scheinberg IH, Hickman J, Ashwell G. The role of sialic acid in determining the survival of glycoproteins in the circulation. J Biol Chem 1971; 246:1461–1467.PubMedGoogle Scholar
  14. 14.
    Fiete D, Srivastava V, Hindsgaul O, Baenziger JU. A hepatic reticuloendothelial cell receptor specific for SO4–4GalNAcbl,4GlcNAcbl,2Mana that mediates rapid clearance of lutropin. Cell 1991; 67:1103–1110.PubMedGoogle Scholar
  15. 15.
    Baenziger JU, Kumar S, Brodbeck RM, Smith PL, Beranek MC. Circulatory half-life but not interaction with the lutropin/chorionic gonadotropin receptor is modulated by sulfation of bovine lutropin oligosaccharides. Proc Natl Acad Sci USA 1992; 89:334–338.PubMedGoogle Scholar
  16. 16.
    Veldhuis JD, Johnson ML. In Vivo dynamics of luteinizing hormone secretion and clearance in man: assessment by deconvolution mechanics. J Clin Endocrinol Metab. 1988; 66: 1291–1300.PubMedGoogle Scholar
  17. 17.
    Yen SSC, Llerena O, Little B, Pearson OH. Disappearance rates of endogenous luteinizing hormone and dhorionic gonadotropin in man. J Clin Endocrinol Metab. 1968; 28:1763–1767.PubMedGoogle Scholar
  18. 18.
    Urban RJ, Padmanabhan V, Beitins I, Veldhuis JD. Metabolic clearance of human follicle-stimulating hormone assessed by radioimmunoassay, immunoradiometric assay, and in Vitro Sertoli cell bioassay. J Clin Endocrinol Metab 1991; 73:818–823.PubMedGoogle Scholar
  19. 19.
    Yen SSC, Llerena LA, Pearson OH, Littell AS. Disappearance rates of endogenous follicle-stimulating hormone in serum following surgical hypophysectomy in man. J Clin Endocrinol. 1970; 30:325–329.Google Scholar
  20. 20.
    Matzuk MM, Boime I. Mutagenesis and gene transfer define site-specific roles of the gonadotropin oligosacharides. Biol Repro. 1989; 40:48–53.Google Scholar
  21. 21.
    Sairam MR, Bhargavi GN. A role for the glycosylation of the alpha-subunit in the transduction of biological signal in glycoprotein hormones. Science 1985; 229:65–67.PubMedGoogle Scholar
  22. 22.
    Keutmann HT, Mcllroy PJ, Bergert ER, Ryan RJ. Chemically deglycosylated human chorionic gonadotropin subunits: characterization and biological properties. Biochemistry 1983; 22:3067–3072.PubMedGoogle Scholar
  23. 23.
    Stanton PG, Pozvek G, Burgon PG, Robertson DM, Hearn MTW. Isolation and characterization of human LH isoforms. J Endocrinol 1993; 138:529–543.PubMedGoogle Scholar
  24. 24.
    Strollo F, Harlin J, Hernandez-Montes H, Robertson DM, Zaidi AA, Diczfalusy E. Qualitative and quantitative differences in the isoelectrofocusing profile of biologically active lutropin in the blood of normally menstruating and post-menopausal women. Acta Endocrinol 1981; 97: 166–175.PubMedGoogle Scholar
  25. 25.
    Lucky AW, Rich BH, Rosenfield RL, Fang VS, Roche-Bender N. LH bioactivity increases more than immunoreactivity during puberty. J Ped 1980; 97:205–513.Google Scholar
  26. 26.
    Keutmann HT. Receptor-binding regions in human glycoprotein hormones. Mol Cell Endocrinol 1992; 86:C1–C6.PubMedGoogle Scholar
  27. 27.
    Lapthorn AJ, Harris DC, Littlejohn A et al. Crystal structure of human chorionic gonadotropin. Nature 1994;369:455–461.PubMedGoogle Scholar
  28. 28.
    Matsuo H, Baba Y, Nair RM, Arimura A, Schally AV. Structure of the porcine LH- and FSH-releasing hormone. I. The proposed amino acid sequence. Biochem Biophys Res Commun 1971; 43:1334–1339.PubMedGoogle Scholar
  29. 29.
    McCann SM, Mizunuma H, Samson WK. Differential hypothalamic control of FSH secretion: a review. Psychoneuroendocrinol 1983; 8:299–308.Google Scholar
  30. 30.
    Carroll RS, Corrigan AZ, Gharib SD, Vale W, Chin WW. Inhibin, activin, and follistatin: regulation of follicle-stimulating hormone messenger ribonucleic acid levels. Mol Endocrinol 1989; 3:1969–1976.PubMedGoogle Scholar
  31. 31.
    Rivier C, Rivier J, Vale W. Inhibin-mediated feedback control of follicle-stimulating hormone secretion in the female rat. Science 1986; 234:205–208.PubMedGoogle Scholar
  32. 32.
    Majumdar SS, Mikuma N, Ishwad PC et al. Replacement with recombinant human inhibin immediately after orchidectomy in the hypophysiotropically clamped male rhesus monkey (Macaca mulatta) maintains follicle-stimulating hormone (FSH) secretion and FSH beta messenger ribonucleic acid levels at precastration values. Endocrinology 1995; 136:1969–1977.PubMedGoogle Scholar
  33. 33.
    Christensen RB, Forage RG, Steiner RA, Bremner WJ. Effects of castration and recombinant human inhibin administration on circulating levels of inhibin and gonadotropins in adult male monkeys. J Andrology 1994; 15:5–31.Google Scholar
  34. 34.
    DePaolo LV, Shimonaka M, Schwall RH, Ling N. In vivo comparison of the follicle-stimulating hormone-suppressing activity of follistatin and inhibin in ovariectomized rats. Endocrinology 1991; 128:668–674.PubMedGoogle Scholar
  35. 35.
    Meunier H, Rivier C, Evans RM, Vale W Gonadal and extragonadal expression of inhibin alpha, beta-A, and beta-B subunits in various tissues predicts diverse functions. Proc Natl Acad Sci 1988; 85:247–251.PubMedGoogle Scholar
  36. 36.
    Weiss J, Harris PE, Halvorson LM, Crowley WF, Jr., Jameson JL. Dynamic regulation of follicle-stimulating hormone-beta messenger ribonucleic acid levels by activin and gonadotropin-releasing hormone in perifused rat pituitary cells. Endocrinology 1992; 131:1403–1408.PubMedGoogle Scholar
  37. 37.
    Roberts V, Meunier H, Vaughan J, et al. Production and regulation of inhibin subunits in pituitary gonadotropes. Endocrinology 1989; 124:552–554.PubMedGoogle Scholar
  38. 38.
    Kaiser UB, Lee BL, Carroll RS, Unabia G, Chin WW, Childs GV. Follistatin gene expression in the pituitary: localization in gonadotropes and folliculostellate cells in diestrous rats. Endocrinology 1992; 130:3048–3056.PubMedGoogle Scholar
  39. 39.
    Kogawa K, Nakamura T, Sugino K, Takio K, Titani K, Sugino H. Activin-binding protein is present in the pituitary. Endocrinology 1991; 128:1434–1440.PubMedGoogle Scholar
  40. 40.
    Halvorson LM, Weiss J, Bauer-Dantoin AC, Jameson JL. Dynamic regulation of pituitary follistatin messenger ribonucleic acids during the rat estrous cycle. Endocrinology 1994; 134:1247–1253.PubMedGoogle Scholar
  41. 41.
    Kirk SE, Dalkin AC, Yasin M, Haisenleder DJ, Marshall JC. Gonadotropin-releasing hormone pulse frequency regulates expression of pituitary follistatin messenger ribonucleic acid: a mechanism for differential gonadotrope function. Endocrinology 1994; 135:876–880.PubMedGoogle Scholar
  42. 42.
    Belchetz PE, Plant TM, Nakai Y, Keogh EJ, Knobil E. Hypophysial responses to continuous and intermittent delivery of hypopthalamic gonadotropin-releasing hormone. Science 1978; 202:631–633.PubMedGoogle Scholar
  43. 43.
    Stojilkovic SS, Reinhart J, Catt KJ. Gonadotropin-releasing hormone receptors: structure and signal transduction pathways. Endocrine Rev 1994; 15:462–499.Google Scholar
  44. 44.
    Levine JE, Pau KY, Ramirez VD, Jackson GL. Simultaneous measurement of luteinizing hormone-releasing hormone and luteinizing hormone release in unanesthetized, ovariectomized sheep. Endocrinology 1982; 111:.Google Scholar
  45. 45.
    Clarke IJ, Cummins JT. The temporal relationship between gonadotropin releasing hormone (GnRH) and luteinizing hormone (LH) secretion in ovariectomized ewes. Endocrinology 1982; 111: 1737–1739.PubMedGoogle Scholar
  46. 46.
    Marshall JC, Dalkin AC, Haisenleder DJ, Paul SJ, Ortolano GA, Kelch RP Gonadotropin-releasing hormone pulses: regulators of gonadotropin synthesis and ovulatory cycles. [Review]. Rec Prog Horm Res 1991;47:155–187.PubMedGoogle Scholar
  47. 47.
    Crowley WF, Jr., Whitcomb RW, Jameson JL, Weiss J, Finkelstein JS, O’Dea LS. Neuroendocrine control of human reproduction in the male. [Review]. Rec Prog Horm Res 1991; 47:27–62.PubMedGoogle Scholar
  48. 48.
    Winters SJ, Troen P. Pulsatile secretion of immunoreactive alpha-subunit in man. J Clin Endocrinol Metab 1985; 60:344–348.PubMedGoogle Scholar
  49. 49.
    Marshall JC, Kelch RP Gonadotropin-releasing hormone: role of pulsatile secretion in the regulation of reproduction. N Engl J Med 1986; 315:1459–1468.PubMedGoogle Scholar
  50. 50.
    Knobil E. Patterns of hormonal signals and hormone action. N Engl J Med 1981; 305:1582, 1583.PubMedGoogle Scholar
  51. 51.
    Handelsman DJ, Boylan LM. Pharmacodynamics of gonadotropin-releasing hormone (GnRH). II. Pattern of GnRH delivery alters pituitary luteinizing hormone secretion in women. J Clin Endocrinol Metab 1988; 67:175–179.PubMedGoogle Scholar
  52. 52.
    Gross KM, Matsumoto AM, Bremner WJ. Differential control of luteinizing hormone and follicle-stimulating hormone secretion by luteinizing hormone-releasing hormone pulse frequency in man. J Clin Endocrinol Metab 1987; 64:675–680.PubMedGoogle Scholar
  53. 53.
    Gross KM, Matsumoto AM, Berger RE, Bremner WJ. Increased frequency of pulsatile luteinizing hormone-releasing hormone administration selectively decreases follicle-stimulating hormone levels in men with idiopathic azoospermia. Fert. Sterility 1986; 45:392–396.Google Scholar
  54. 54.
    Wildt L, Hausier A, Marshall G, et al. Frequency and amplitude of gonadotropin-releasing hormone stimulation and gonadotropin secretion in the rhesus monkey. Endocrinology 1981; 109:376–385.PubMedGoogle Scholar
  55. 55.
    Haisenleder DJ, Dalkin AC, Ortolano GA, Marshall JC, Shupnik MA. A pulsatile gonadotropin-releasing hormone stimulus is required to increase transcription of the gonadotropin subunit genes: evidence for differential regulation of transcription by pulse frequency in vivo. Endocrinology 1991; 128:509–517.PubMedGoogle Scholar
  56. 56.
    Nippoldt TB, Reame NE, Kelch RP, Marshall JC. The roles of estradiol and progesterone in decreasing luteinizing hormone pulse frequency in the luteal phase of the menstrual cycle. J Clin Endocrinol Metab 1989; 69:67–76.PubMedGoogle Scholar
  57. 57.
    Hall JE, Schoenfeld DA, Martin KA, Crowley WF, Jr. Hypothalamic gonadotropin-releasing hormone secretion and follicle-stimulating hormone dynamics during the luteal-follicular transition. J Clin Endocrinol Metab 1992; 74:600–607.PubMedGoogle Scholar
  58. 58.
    Spratt DI, Finkelstein JS, Butler JP, Badger TM, Crowley WF, Jr. Effects of increasing the frequency of low doses of gonadotropin-releasing hormone (GnRH) on gonadotropin secretion in GnRH-deficient men. J Clin Endocrinol Metab 1987; 64: 1179–1186.PubMedGoogle Scholar
  59. 59.
    Moenter SM, Caraty A, Locatelli A, Karsch FJ. Pattern of gonadotropin-releasing hormone (GnRH) secretion leading up to ovulation in the ewe: existence of a preovulatory GnRH surge. Endocrinology 1991; 129:1175–1182.PubMedGoogle Scholar
  60. 60.
    Caraty A, Antoine С, Delaleu В, et al. Nature and bioactivity of gonadotropin-releasing hormone (GnRH) secreted during the GnRH surge. Endocrinology 1995; 136:3452–3460.PubMedGoogle Scholar
  61. 61.
    Kaplan SL, Grumbach MM. The ontogenesis of human foetal hormones. II. Luteinizing hormone (LH) and follicle stimulating hormone (FSH). Acta Endocrinol 1976; 81:808–829.PubMedGoogle Scholar
  62. 62.
    Castillo RH, matteri RL, Dumesic DA. Luteinizing hormone synthesis in cultured fetal human pituitary cells exposed to gonadotropin-releasing hormone. J Clin Endocrinol Metab. 1992; 75:318–322.PubMedGoogle Scholar
  63. 63.
    Resko JA, Ellinwood WE. Negative feedback regulation of gonadotropin secretion by androgens in fetal rhesus macaques. Biol Repro 1985; 33:346–352.Google Scholar
  64. 64.
    Rabinovici J, Goldsmith PC, Roberts VJ, Vaughan J, Vale W, Jaffe RB. Localization and secretion of inhibin/activin subunits in the human and. J Clin Endocrinol Metab 1991; 73:1141–1149.PubMedGoogle Scholar
  65. 65.
    Rannikki AS, Zhang FP, Huhtaniemi IT. Ontogeny of follicle-stimulating hormone receptor gene expression in the rat testis and ovary. Mol Cell Endocrinol 1995; 107:199–208.PubMedGoogle Scholar
  66. 66.
    Waldhauser F, Weissenbacher G, Frisch H, Pollak A. Pulsatile secretion of gonadotropins in early infancy. Eur J Pediatr 1981; 137:71–74.PubMedGoogle Scholar
  67. 67.
    Jakacki RI, Kelch RP, Sauder SE, Lloyd JS, Hopwood NJ, Marshall JC. Pulsatile secretion of luteinizing hormone in children. J Clin Endocrinol Metab 1982; 55:453–58.PubMedGoogle Scholar
  68. 68.
    Terasawa E. Control of luteinizing hormone-releasing hormone pulse generation in nonhuman primates. [Review]. Cell Molecular Neurobiol 1995; 15:141–164.Google Scholar
  69. 69.
    Bourguignon JP, Gerard A, Alvarez GML, Franchimont P. Control of pulsatile secretion of gonadotrophin releasing hormone from hypothalamic expiants. [Review]. Human Reprod 1993; 2:18–22.Google Scholar
  70. 70.
    Boyar R, Finkelstein J, Roffwarg H, Kapen S, Weitzman E, Hellman L. Synchronization of augmented luteinizing hormone secretion with sleep during puberty. N Engl J Med 1972; 287:582–586.PubMedGoogle Scholar
  71. 71.
    Lucky AW, Rich BH, Rosenfield RL, Fang WS, Roche-Bender N. LH bioactivity increases more than immunoreactivity during puberty. J Ped 1980; 97:205–213.Google Scholar
  72. 72.
    Sherman BM, Korenman SG. Hormonal characteristics of the human menstrual cycle throughout reproductive life. J Clin Inv 1975; 55:699–706.Google Scholar
  73. 73.
    Zumoff B, Strain GW, Kream J, et al. Age variation of the 24–hour mean plasma concentrations of androgens, and gonadotropins in normal adult men. J Clin Endocrinol Metab 1982; 54:534–538.PubMedGoogle Scholar
  74. 74.
    Veldhuis JD, Urban RJ, Lizarralde G, Johnson ML, Iranmesh A. Attenuation of luteinizing hormone secretory burst amplitude as a proximate basis for the hypoandrogenism of healthy aging in men. J Clin Endocrinol Metab 1992; 75:707–713.PubMedGoogle Scholar
  75. 75.
    Dong J, Nishimori K, Kumar TR, Wang Y, Lu N, Guo Q, Matzuk MM. Transgenic mouse models to study folliculogenesis. Abstract. Int Symp on Life Cycle of the Ovarian Follicle 1995; Ft Lauderdale.Google Scholar
  76. 76.
    Sutherland EW, Robinson GA. Role of cyclic 3′-5′AMP in response to catecholamines and other hormones. Pharmacol. Rev 1966; 18:145–161.PubMedGoogle Scholar
  77. 77.
    Loosfelt H, Misrahi M, Atger M, et al. Cloning and sequencing of porcine LH-hCG receptor cDNA: variants lacking transmembrane domain. Science 1989; 245:525–528.PubMedGoogle Scholar
  78. 78.
    McFarland KC, Sprengel R, Phillips HS, et al. Lutropin-choriogonadotropin receptor: an unusual member of the G protein-coupled receptor family. Science 1989; 245:494–499.PubMedGoogle Scholar
  79. 79.
    Sprengel L, Braun T, Nikolics K, Segaloff DL, Seeburg PH. The testicular receptor for follicle stimulating hormone: structure and functional expression of cloned cDNA. Mol Endocrinol 1990; 4:525–530.PubMedGoogle Scholar
  80. 80.
    Leung PCK, Steele GL. Intracellular signaling in the gonads. Endocrine Rev 1992; 13:476–498.Google Scholar
  81. 81.
    Tsai-Morris CH, Buczko E, Wang W, Xie XZ, Dufau ML. Structural organization of the rat luteinizing hormone (LH) receptor gene. J Biol Chem 1991; 266:11355–11359.PubMedGoogle Scholar
  82. 82.
    Heckert LL, Daley IJ, Griswold MD. Structural organization of the follicle-stimulating hormone receptor gene. Mol Endocrinol 1992; 6:70–80.PubMedGoogle Scholar
  83. 83.
    Segaloff DL, Ascoli M. The lutropin/choriogonadotropin receptor… 4 years later. Endocrine Rev 1993; 14:324–347.Google Scholar
  84. 84.
    Lei ZM, Rao CV, Kornyei JL, Licht P, Hiatt ES. Novel expression of human chorionic gonadotropin/luteinizing hormone receptor gene in brain. Endocrinology 1993; 132:2262–2270.PubMedGoogle Scholar
  85. 85.
    Mellon PL, Windle JJ, Goldsmith PC, Padula CA, Roberts JL, Weiner RI. Immortalization of hypothalamic GnRH neurons by genetically targeted tumorigenesis. Neuron 1990; 5:1–10.PubMedGoogle Scholar
  86. 86.
    Lei ZM, Rao CV. Novel presence of luteinizing hormone/human chorionic gonadotropin (hCG) receptors and the down-regulating action of hCG on gonadotropin-releasing hormone gene expression in immortalized hypothalamic GT1–7 neurons. Mol Endocrinol 1994; 8:1111–1121.PubMedGoogle Scholar
  87. 87.
    Melrose PA. In vitro evidence for short-loop gonadotropin feedback on gonadotropin-releasing hormone neurons harvested from adult male rats. Endocrinology 1987; 121:200–204.PubMedGoogle Scholar
  88. 88.
    Padmanabhan V, Evans NP, Dahl GE, McFadden KL, Mauger DT, Karsch FJ. Evidence for short or ultrashort loop negative feedback of gonadotropin-releasing hormone secretion. Neuroendocrinology 1995; 62:248–258.PubMedGoogle Scholar
  89. 89.
    Geola FL, Frumar AM, Tataryn IV, et al. Biological effects of various doses of conjugated equine estrogens in postmenopausal women. J Clin Endocrinol Metab 1980; 51:620–625.PubMedGoogle Scholar
  90. 90.
    Finkelstein JS, O’Dea LS, Whitcomb RW, Crowley WF, Jr. Sex steroid control of gonadotropin secretion in the human male. II. Effects of estradiol administration in normal and gonadotropin-releasing hormone-deficient men. J Clin Endocrinol Metab 1991; 73:621–628.PubMedGoogle Scholar
  91. 91.
    Finkelstein JS, Whitcomb RW, O’Dea LS, Longcope C, Schoenfeld DA, Crowley WF, Jr. Sex steroid control of gonadotropin secretion in the human male. I. Effects of testosterone administration in normal and gonadotropin-releasing hormone-deficient men. J Clin Endocrinol Metab 1991; 73:609–620.PubMedGoogle Scholar
  92. 92.
    Bagatell CJ, Dahl KD, Bremner WJ. The direct pituitary effect of testosterone to inhibit gonadotropin secretion in men is partially mediated by aromatization to estradiol. J Andrology 1994; 15: 15–21.Google Scholar
  93. 93.
    Moenter SM, Caraty A, Locatelli A, Karsch FJ. Pattern of gonadotropin-releasing hormone (GnRH) secretion leading up to ovulation in the ewe: existence of a preovulatory GnRH surge. Endocrinology 1991; 129:1175–1182.PubMedGoogle Scholar
  94. 94.
    Kolp LA, Pavlou SN, Urban RJ, Rivier JC, Vale WW, Veldhuis JD. Abrogation by a potent gonadotropin-releasing hormone antagonist of the estrogen/progesterone-stimulated surge-like release of luteinizing hormone and follicle-stimulating hormone in postmenopausal women. J Clin Endocrinol Metab 1992; 75:993–997.PubMedGoogle Scholar
  95. 95.
    Shivers BD, Harlan RE, Morrell JI, Pfaff DW Absence of estradiol concentration in cell nuclei of LHRH-immunoreactive neurones. Nature 1983; 304:345–347.PubMedGoogle Scholar
  96. 96.
    Winters SJ, Janick JJ, Loriaux DL, Sherins RJ. Studies on the role of sex steroids in the feedback control of gonadotropin concentrations in men. II. Use of the estrogen antagonist, clomiphen citrate. J Clin Endocrinol Metab 1979; 48:222–227.PubMedGoogle Scholar
  97. 97.
    Sherins RJ, Loriaux DL. Studies on the role of sex steroids in the feedback control of FSH concentrations in men. J Clin Endocrinol Metab 1973; 36:886–893.PubMedGoogle Scholar
  98. 98.
    McCullagh DR. Dual endocrine activity of testes. Science 1932; 76:19.PubMedGoogle Scholar
  99. 99.
    Mason AJ, Hayflick JS, Ling N, et al. Complementary DNA sequences of ovarian follicular fluid inhibin show precursor structure and homology with transforming growth factor-beta. Nature 1985; 318:659–663.PubMedGoogle Scholar
  100. 100.
    Vale W, Rivier C, Hsueh A. Chemical and biological characterization of the inhibin family of protein hormones. Rec Prog Horm Res 1988; 44:1–34.PubMedGoogle Scholar
  101. 101.
    McLachlan RI, Robertson DM, Burger HG, de KDM. The radioimmunoassay of bovine and human follicular fluid and serum inhibin. Mol Cell Endocrinol 1986; 46:175–185.Google Scholar
  102. 102.
    Schneyer AL, Mason AJ, Burton LE, Ziegner JR, Crowley WF, Jr. Immunoreactive inhibin alpha-subunit in human serum: implications for radioimmunoassay. J Clin Endocrinol Metab 1990; 70: 1208–1212.PubMedGoogle Scholar
  103. 103.
    DeKretser DM, McLachlan RI, Robertson DM, Burger HG. Serum inhibin levels in normal men and men with testicular disorders. J Clin Endocrinol Metab 1989; 70:1414–1419.Google Scholar
  104. 104.
    Groome N, O’Brien M. Immunoassays for inhibin and its subunits: further applications of the synthetic peptide approach. J Immunol Methods 1993; 165:167–176.PubMedGoogle Scholar
  105. 105.
    Lambert-Messerlian GM, Hall JE, Sluss PM, et al. Relatively low levels of dimeric inhibin circulate in men and women with polycystic ovarian syndrome using a specific two-site enzyme-linked immunosorbent assay. J Clin Endocrinol Metab 1994; 79:45–5.PubMedGoogle Scholar

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© Humana Press Inc. 1997

Authors and Affiliations

  • François P. Pralong
  • William F. CrowleyJr.

There are no affiliations available

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