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Cellular Toxicology

  • M. Duane Enger
Chapter
Part of the Reviews of Environmental Contamination and Toxicology book series (RECT, volume 102)

Abstract

Cultured cells complement animals as experimental and test subjects in basic and applied toxicology. Their use presents obvious advantages as well as disadvantages, which in both instances are ascribable to their uniformity and simplicity relative to experimental animals. Commonly used animal cells are available from central repositories, such as the American Type Culture Collection and the Camden Repository (NIGMS Human Mutant Cell Repository). They can be established in culture relatively quickly and require little in the way of space and instrumentation for maintenance and use. Commercial sources provide prepared growth media and sera or growth factors. Once established in culture, they may be exposed to well-defined and known concentrations of toxic agents and can be as readily removed from such exposure or switched to another chemical environment or growth state. A large number of replicates are easily accommodated. Ease of manipulation and exact definition of exposure thus represent big advantages. The time and cost incurred relative to that associated with animal experimentation are much less. Also, the effects of a toxicant may be ascribed to direct action, as the opportunities for indirect effects due to altered neurological, hormonal, or immune functions do not exist.

Keywords

Gene Amplification Intercellular Communication Normal Human Bronchial Epithelial Buthionine Sulfoximine Cell BioI 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag New York Inc. 1988

Authors and Affiliations

  • M. Duane Enger
    • 1
  1. 1.Zoology DepartmentIowa State UniversityAmesUSA

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