Cruciform Transitions Assayed Using a Psoralen Crosslinking Method: Applications to measurements of DNA torsional tension
It is now established that cruciform structures can form in supercoiled DNA at sites of inverted repeat sequences. Several different methods have been developed for detecting cruciforms, including: scission of the DNA with nucleases specific for the unpaired bases at the termini of cruciform arms (Lilley, 1980, 1981; Panayotatos et al; Singleton et al,); scission with restriction enzymes (Geliert et al; Courey et al); electrophoretic resolution of DNA containing or not containing a cruciform (Mizuuchi et al; Geliert et al); electron microscopic observation (Mizuuchi et al).
KeywordsEnthalpy EDTA Agarose Bromide Electrophoresis
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