Skip to main content

Radioimmunoassays and ELISAs: Peptides

  • Chapter
Immunological Techniques in Insect Biology

Part of the book series: Springer Series in Experimental Entomology ((SSEXP))

Abstract

It is hardly an exaggeration to state that the introduction of the radioimmunoassay (RIA) more than 25 years ago has been the single most important methodological advance in endocrinology in the past 50 years. The ability to measure notoriously small amounts of hormone in blood samples has decisively shaped our current concepts regarding endocrine mechanisms, especially in vertebrates. The impact of this technique on invertebrate endocrinology is of a more recent date. The introduction of the ecdysone RIA 16 years ago (Borst and O’Connor, 1972; see Chapter 6) has had enormous consequences for the development of arthropod endocrinology.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 84.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Reference

  • Abramowitz, A.A., Hisaw, F.L., and Papandrea, D.N. (1944). The occurrence of a diabetogenic factor in the eyestalk of crustaceans. Biol. Bull. 86:1–5.

    Article  CAS  Google Scholar 

  • Beenakkers, A.T.M. (1983). Regulation of lipid metabolism. In: Invertebrate Endocrinology Vol, I. Endocrinology of Insects. R.G.H. Downer and H. Laufer (Eds.). Alan R. Liss, NewYork, pp. 441–450.

    Google Scholar 

  • Benoit, R., Bohlen, P., Ling, N., et al. (1982). Presence of somatostatin-28-(1–12) in hypothalamus and pancreas. Proc. Natl. Acad. Sci. USA 79:917–921.

    Article  PubMed  CAS  Google Scholar 

  • Benson, J.A., Sullivan, P.T., Watson, W.N., III, and Augustine, G.J. Jr. (1981). The neuropeptide proctolin acts directly on Limulus cardiac muscle to increase the amplitude of contraction. Brain Res. 213:449–454.

    Article  PubMed  CAS  Google Scholar 

  • Borst, D.W., and O’Connor, J.D. (1972). Arthropod molting hormone: Radioimmune assay. Science 178:418–419.

    Article  PubMed  CAS  Google Scholar 

  • Bishop, C.A., O’Shea, M., and Miller, R.J. (1981). Neuropeptide proctolin: Immuno-cytochemical detection and neuronal localization in insect central nervous system. Proc. Natl. Acad. Sci. USA 78:5899:5902.

    Article  PubMed  CAS  Google Scholar 

  • Dircksen, H., Zahnow, C., Gaus, G., Keller, R., Rao, K.R., and Riehm, I.P. (1987). The ultrastructure of nerve endings containing pigment dispersing hormone (PDH) in crustacean sinus glands identified by an antiserum against a synthetic PDH. Cell Tissue Res. 250:377–387.

    Article  CAS  Google Scholar 

  • Fernlund, P. (1976). Structure of a light adapting hormone from the shrimp, Pandalus borealis. Biochim. Biophys. Acta 439:17–25.

    CAS  Google Scholar 

  • Frechet, P., Roth, J., and Neville, D.M. (1971). Monoiodoinsulin: Demonstration of its biological activity and binding to fat cells and liver membranes. Biochem. Biophys. Res. Commun. 43:400–405.

    Article  Google Scholar 

  • Holets, Y.R., Hokfelt, J., Ude, J., Eckert, M., and Hansen, S. (1984). Coexistence of proctolin with TRH and 5-HT in the rat CNS. Soc, Neurosci. Abstr. 10(2): 692.

    Google Scholar 

  • Keller, R., and Kegel, G, (1984). Studies on crustacean neuropeptides by use of high performance liquid chromatography. In: Biosynthesis, Metabolism and Mode of Action of Invertebrate Hormones. J.A. Hoffmann and M. Porchet (Eds.). Springer-Verlag, Heidelberg, pp. 145–154.

    Google Scholar 

  • Keller, R., and Sedlmeier, D., (1987). A metabolic hormone in crustaceans: The hyperglycemic neuropeptides. In: Invertebrate Endocrinology Vol. II. R.G.H. Downer and H. Laufer (Eds.). Alan R. Liss, New York (in press).

    Google Scholar 

  • Keller, R., Jaros, P.P., and Kegel, G., (1985). Crustacean hyperglycemic neuropeptides. Am. Zool. 25:207–221.

    CAS  Google Scholar 

  • Kingan, T., and Titmus, M. (1983). Radioimmunologic detection of proctolin in arthropods. Comp. Biochem. Physiol. 74C: 75–78.

    CAS  Google Scholar 

  • Kishida, Y., Olsen, B.R., Berg, R.A., and Prockop, D.J. (1975). Two improved methods for preparing ferritin-protein conjugates for electron microscopy. J. Cell Biol. 64:331–339.

    Article  PubMed  CAS  Google Scholar 

  • Kleinholz, L.H., and Keller, R. (1973). Comparative studies in crustacean neurosecretory hyperglycemic hormones. I. The initial survey. Gen. Comp. Endocrinol. 21:554–564.

    Article  PubMed  CAS  Google Scholar 

  • Kleinholz, L.H., and Keller, R. (1979). Endocrine regulation in crustacea. In: Hormones and Evolution, Vol. I. E.J.W. Barrington (Ed.). Academic Press, New York, pp. 159–214.

    Google Scholar 

  • Kleinholz, L.H., Rao, K.R., Riehm, J.P., Tarr, G.E., Johnson, L., and Norton, S. (1986). Isolation and sequence analysis of a pigment dispersing hormone from eyestalks of the crab, Cancer magister. Biol. Bull. 170:135–143.

    Article  Google Scholar 

  • Kopec, S. (1922). Studies on the necessity of the brain for the inception of insect metamorphosis. Biol. Bull. Woods Hole 42:322–342.

    Article  Google Scholar 

  • Mangerich, S., Keller, R., Dircksen, H., Rao, K.R., and Riehm, J.P. (1987). Localization of pigment-dispersing hormone (PDH) and coexistence with FMRFamide immunoreactivity in the eyestalks of two decapod crustaceans. Cell Tissue Res. 250:365–375.

    Article  Google Scholar 

  • Martin, R., and Voigt, K.H. (1987). The neurosecretory system of the Octopus vena cava: A neurophemal organ. Experientia 43:537–543.

    Article  CAS  Google Scholar 

  • Moshitzky, P., Yamashiro, D., Stuve, L., Ramachandran, J., and Applebaum, S.W. (1987). Determination of locust AKH-I by radioimmunoassay and the identification of an AKH-like factor in the locust brain. Insect Biochem. 17:765–769.

    Article  CAS  Google Scholar 

  • Nagasawa, H., Kataoka, H., Isogai, A., et al. (1986). Amino acid sequence of prothoracicotropic hormone of the silkworm Bombyx mori. Proc. Natl. Acad. Sci. USA 83:5840–5843.

    Article  PubMed  CAS  Google Scholar 

  • Quackenbush, L.S., and Fingerman, M, (1985). Enzyme-linked immunosorbent assay of black pigment dispersing hormone from the fiddler crab, Uca pugilator. Gen. Comp. Endocrinol. 57:438–445.

    Article  CAS  Google Scholar 

  • Rao, K.R. (1985). Pigmentary effectors. In: The Biology of Crustacea, Vol. 9. D.E. Bliss and L.H. Mantel (Eds.). Academic Press, New York, pp. 395–462.

    Google Scholar 

  • Rao, K.R., Riehm, J.R., Zahnow, C.A., et al. (1985). Characterization of a pigment-dispersing hormone in eyestalks of the fiddler crab Uca pugilator. Proc. nail Acad. Sci USA 82:5319–5323.

    Article  PubMed  CAS  Google Scholar 

  • Schooneveld, H., and Tesser, G.I. (1983). Adipokinetic hormone and AKH-like peptide demonstrated in the corpora cardiaca and nervous system of Locusta migratoria by immunocytochemistry. Cell Tissue Res. 230:67–76.

    Article  PubMed  CAS  Google Scholar 

  • Schooneveld, H., and Veenstra, J.A. (1985). Insect neuroendocrine cells and neurons containing various adipokinetic hormone (AKH)-immunoreactive substances. In: Neurosecretion and the Biology of Neuropeptides. H. Kobayashi et al., (Eds.). Japan Science Society press, Tokyo, pp. 425–434.

    Google Scholar 

  • Schwarz, T.L., Lee, G.M.M., Siwicki, K., Standaert, D.G., and Kravitz, E.A. (1984). Proctolin in the lobster: The distribution, release;and chemical characterization of a likely neurohormone. J. Neurosci. 4:1300–1311.

    PubMed  CAS  Google Scholar 

  • Siegert, K., Morgan, P., and Mordue, W. (1985). Primary structures of locust adipokinetic hormones II. Biol. Chem, Hoppe-Seyler 366:723–727.

    Article  CAS  Google Scholar 

  • Siwicki, K.K., and Bishop, C.A. (1986). Mapping of procto linlike immunoreactivity in the nervous systems of lobster and crayfish. J. Comp. Neurol. 243:435–453.

    Article  PubMed  CAS  Google Scholar 

  • Skowsky, W.R., and Fisher, D.A. (1972). The use of thyroglobulin to induce antigenicity to small molecules. J. Lab. Clinf Med. 80:134–144.

    CAS  Google Scholar 

  • Stangier, J., Dircksen, H., and Keller, R. (1986). Identification and immunocytochemical localization of proctolin in pericardial organs of the shore crab, Carcinus maenas. Peptides 7:67–72.

    Article  PubMed  CAS  Google Scholar 

  • Starratt, A.N., and Brown, B.E., (1975). Structure of the pentapeptide proctolin, a proposed neurotransmitter in insects. Life Sci. 17:1253–1256.

    Article  PubMed  CAS  Google Scholar 

  • Stone, J.V., Mordue, W., Batley, K.E., and Morris, H.R. (1976). Structure of locust adipokinetic hormone, a neurohormone that regulates lipid utilisation during flight. Nature 263:207–211.

    Article  PubMed  CAS  Google Scholar 

  • Vaitukaitis, J.L., (1981). Production of antisera with small doses of immunogen: Multiple intradermal injections. In: Methods in Enzymology Vol. 73. J.J. Langone and H. Van Vunakis (Eds.). Academic Press, New York, pp. 46–52.

    Google Scholar 

  • Webster, S.G., and Keller, R. (1986). Purification, characterization and amino acid composition of the putative moult-inhibiting hormone (MIH) of Carcinus maenas (Crustacea, Decapoda). J. Comp. Physiol. B 156:617–624.

    Google Scholar 

Download references

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 1988 Springer-Verlag New York Inc.

About this chapter

Cite this chapter

Keller, R. (1988). Radioimmunoassays and ELISAs: Peptides. In: Gilbert, L.I., Miller, T.A. (eds) Immunological Techniques in Insect Biology. Springer Series in Experimental Entomology. Springer, New York, NY. https://doi.org/10.1007/978-1-4612-3798-3_8

Download citation

  • DOI: https://doi.org/10.1007/978-1-4612-3798-3_8

  • Publisher Name: Springer, New York, NY

  • Print ISBN: 978-1-4612-8356-0

  • Online ISBN: 978-1-4612-3798-3

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics