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Time-Resolved Fluorescence Immunoassay

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Immunological Techniques in Insect Biology

Part of the book series: Springer Series in Experimental Entomology ((SSEXP))

Abstract

For more than 20 years, immunological techniques have been applied extensively to measure hormone titers quantitatively, based on the highly specific and sensitive binding of antigens to antibodies. Originally, radioisotopes were used for labeling the hormone or the antibody to measure the hormone titers via radioimmunoassay (RIA) or immunoradiometric assay (IRMA). Although this approach can still be of great value, applications of radioisotopes involve several disadvantages like potential health and environmental hazards and particularly also the often relatively short half-life of the radiolabel itself and the process of radiolysis causing deterioration of the labeled compounds.

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Authors
  1. Rob C. H. M. Oudejans
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  2. Hans Voshol
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  3. Thomas K. F. Schulz
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  4. Ad M. T. Beenakkers
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Editors and Affiliations

  1. Department of Biology, University of North Carolina, CB 3280, Coker Hall, 27599-3280, Chapel Hill, North Carolina, USA

    Lawrence I. Gilbert

  2. Department of Entomology, University of California, 92521, Riverside, California, USA

    Thomas A. Miller  & Thomas A. Miller  & 

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© 1988 Springer-Verlag New York Inc.

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Oudejans, R.C.H.M., Voshol, H., Schulz, T.K.F., Beenakkers, A.M.T. (1988). Time-Resolved Fluorescence Immunoassay. In: Gilbert, L.I., Miller, T.A. (eds) Immunological Techniques in Insect Biology. Springer Series in Experimental Entomology. Springer, New York, NY. https://doi.org/10.1007/978-1-4612-3798-3_3

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  • DOI: https://doi.org/10.1007/978-1-4612-3798-3_3

  • Publisher Name: Springer, New York, NY

  • Print ISBN: 978-1-4612-8356-0

  • Online ISBN: 978-1-4612-3798-3

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