Spatial Distribution of Chromosomes in Human Mitotic Cells

  • Kenneth W. Adolph

Abstract

The highest level of chromosome organization concerns the threedimensional in vivo arrangement of chromosomes. The 3-D distribution of chromosomes is most readily investigated with mitotic cells, since individual chromosomes can be distinguished, especially in metaphase. The problem being addressed therefore differs from those involving the lower levels of chromosome structure. These relate to the interaction of histones with DNA to form nucleosomes and the coiling of the nucleosome filament into the chromatin fiber. An intermediate level of structure relates to metaphase chromosome substructure—that is, the mode of folding the chromatin fiber into the characteristic morphology of a metaphase chromosome.

Keywords

Citrate Shrinkage Dehydration Glutaraldehyde Uranyl 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Adolph, K.W. (1988) In Chromosomes and Chromatin, Vol. II, 3–27, Adolph, K.W. (ed.). CRC Press, Boca Raton, Florida.Google Scholar
  2. 2.
    Adolph, K.W. (1988) In Chromosomes and Chromatin, Vol. III, 59–85, Adolph, K.W. (ed.). CRC Press, Boca Raton, Florida.Google Scholar
  3. 3.
    Adolph, K.W. (1981) Eur. J. Cell Biol. 24, 146–153.PubMedGoogle Scholar
  4. 4.
    Rosenfeld, A., and Avinash, C.K. (1982) Digital Picture Processing, 261–264. Academic Press, New York.Google Scholar
  5. 5.
    Castleman, K.R. (1979) Digital Image Processing, 316–317. Prentice Hall, New York.Google Scholar
  6. 6.
    Schmid, C.W., and Jelinek, W.R. (1982) Science 216, 1065–1070.PubMedCrossRefGoogle Scholar
  7. 7.
    White, J.H., Cozzarelli, N.R., and Bauer, W.R. (1988) Science 241, 323–327.PubMedCrossRefGoogle Scholar
  8. 8.
    Goodwin, G.H., Nicolas, R.H., Cockerill, P.N., Zavou, S., and Wright, C.A. (1985) Nucleic Acids Res. 13, 3561–3579.PubMedCrossRefGoogle Scholar
  9. 9.
    Balczon, R.D., and Brinkley, B.R. (1987). J. Cell Biol. 105, 855–862.PubMedCrossRefGoogle Scholar
  10. 10.
    Gartier, S.A., and Riggs, A.D. (1983) Annu. Rev. Genet. 17, 155–190.CrossRefGoogle Scholar
  11. 11.
    Hennig, W. (1985) Adv. Genet. 23, 179–234.PubMedCrossRefGoogle Scholar
  12. 12.
    Hotta, Y., and Stern, H. (1984) Chromosoma 89, 127–137.CrossRefGoogle Scholar
  13. 13.
    Busby, S., and Bakken, A.H. (1980) Chromosoma 79, 84–104.CrossRefGoogle Scholar
  14. 14.
    Risley, M.S., Einheber, S., and Bumcrot, D.A. (1986) Chromosoma 94, 217–227.PubMedCrossRefGoogle Scholar
  15. 15.
    Zakian, V.A., Blanton, H.M., and Wetzel, L. (1986) In Extrachromosomal Elements in Lower Eukaryotes, 493–498, Wicker, R.B., Hinnebusch, A., Gunsalus, I.C., Lambowitz, A.M., and Hollaender, A. (eds.). Plenum Press, New York.Google Scholar
  16. 16.
    Jamrich, M. (1986) In Chromosome Structure and Function, 221–242, Risley, M.S. (ed.). Van Nostrand Reinhold, New York.Google Scholar
  17. 17.
    Heslop-Harrison, J.S., Smith, J.B., and Bennett, M.D. (1988) Chromosoma 96, 119–131.CrossRefGoogle Scholar
  18. 18.
    Hochstrasser, M., Mathog, D., Gruenbaum, Y., Saumweber, H., and Sedat, J.W. (1986) J. Cell Biol. 102, 112–123.PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag New York Inc. 1989

Authors and Affiliations

  • Kenneth W. Adolph

There are no affiliations available

Personalised recommendations