Spatial Distribution of Chromosomes in Human Mitotic Cells
The highest level of chromosome organization concerns the threedimensional in vivo arrangement of chromosomes. The 3-D distribution of chromosomes is most readily investigated with mitotic cells, since individual chromosomes can be distinguished, especially in metaphase. The problem being addressed therefore differs from those involving the lower levels of chromosome structure. These relate to the interaction of histones with DNA to form nucleosomes and the coiling of the nucleosome filament into the chromatin fiber. An intermediate level of structure relates to metaphase chromosome substructure—that is, the mode of folding the chromatin fiber into the characteristic morphology of a metaphase chromosome.
KeywordsCitrate Shrinkage Dehydration Glutaraldehyde Uranyl
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