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Ribonucleoproteins and the Structure of Nascent Transcripts

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Molecular Biology of Chromosome Function

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Abstract

Many ultrastructural and biochemical findings suggest that pre-mRNA is packaged during transcription into a repeating array of regular ribonucleoprotein particles by the six “core” proteins of 40S nuclear ribonucleoprotein particles (40S hnRNP particles). If true, then pre-mRNA is packaged in a fashion not totally dissimilar to the nucleosomal packaging of DNA. While the packaging of nascent transcripts into a repeating “ribonucleosome” structure [1,2] seems to complicate our present view of RNA splicing (i.e., splice site recognition, spliceosome assembly, lariat formation, excision, and ligation), there is considerable evidence that all these events do occur while RNA exists in a highly packaged state. On this point it is worth noting that biological systems have worked through the structural and sequence recognition problems created by the packaging of DNA into nucleosomes. More important, it now seems likely that the events of RNA processing can be understood in mechanistic detail because much progress has recently been made in the ability to isolate and study the fundamental repeating element of nascent transcripts and to monitor the biochemical events of RNA packaging in vitro. A better knowledge of monoparticle structure is a prerequisite to a detailed understanding of the events of RNA processing and will help to clarify present attempts to “characterize” the spliceosome complex, a structure quite different from the 40S hnRNP particles that function to package the bulk of nascent pre-mRNA transcripts and prevent the formation of heteroduplex RNA.

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Authors
  1. Stanley F. Barnett
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  2. Stephanie J. Northington
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  3. Wallace M. LeStourgeon
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Editor information

Editors and Affiliations

  1. Department of Biochemistry, University of Minnesota Medical School, Minneapolis, MN, 55455, USA

    Kenneth W. Adolph

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Barnett, S.F., Northington, S.J., LeStourgeon, W.M. (1989). Ribonucleoproteins and the Structure of Nascent Transcripts. In: Adolph, K.W. (eds) Molecular Biology of Chromosome Function. Springer, New York, NY. https://doi.org/10.1007/978-1-4612-3652-8_12

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  • DOI: https://doi.org/10.1007/978-1-4612-3652-8_12

  • Publisher Name: Springer, New York, NY

  • Print ISBN: 978-1-4612-8192-4

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