Skip to main content
SpringerLink
Log in

The Mechanism and Control of Pre-mRNA Splicing

  • Chapter
Molecular Biology of Chromosome Function

  • 121 Accesses

Abstract

Gene expression in eukaryotes requires that primary transcripts of structural genes be modified in a variety of ways before translation as mature mRNAs. One of the most demanding, in terms of mechanism, of these required modifications is the process of splicing, by which noncontiguous stretches of sequences (exons) are brought into juxtaposition with the removal of intervening sequences (introns). Since splicing was discovered several years ago [1,2], enormous strides have been made in the progress of our understanding of this exacting process. This progress has encompassed the basic mechanism or pathway of the reactions involved, the sequences within the pre-mRNA that direct splicing, the factors required and their interactions with the primary transcript, and, recently, the mechanism and control of alternative splicing. References 3 and 4 present related reviews.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
eBook
USD 16.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 16.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Similar content being viewed by others

References

  1. Berget, S.M., Moore, C., and Sharp, P.A. (1977) Proc. Natl. Acad. Sci. USA 74, 3171–3175.

    PubMed  CAS  Google Scholar 

  2. Chow, L.T., Gelinas, R.E., Broker, T.R., and Roberts, R.J. (1977) Cell 12, 1–8.

    PubMed  CAS  Google Scholar 

  3. Green, M.R. (1986) Annu. Rev. Genet. 20, 671–708.

    PubMed  CAS  Google Scholar 

  4. Padgett, R.A., Grabowski, P.J., Konarksa, M.M., Sellers, S., and Sharp, P.A. (1986) Annu. Rev. Biochem. 55, 898–903.

    Google Scholar 

  5. Padgett, R.A., Hardy, S.F., and Sharp, P.A. (1983) Proc. Natl. Acad. Sci. USA 80, 5230–5234.

    PubMed  CAS  Google Scholar 

  6. Hernandez, N., and Keller, W. (1983) Cell 35, 89–99.

    PubMed  CAS  Google Scholar 

  7. Krainer, A.R., Maniatis, T., Ruskin, B., and Green, M.R. (1984) Cell 36, 993–1005.

    PubMed  CAS  Google Scholar 

  8. Lin, R.-J., Newman, A.J., Cheng, S.-C., and Abelson, J. (1985) J. Biol. Chem. 260, 14780–14792.

    PubMed  CAS  Google Scholar 

  9. Grabowski, P., Padgett, R.A., and Sharp, P.A. (1984) Cell 37, 415–427.

    PubMed  CAS  Google Scholar 

  10. Ruskin, B., Krainer, A.R., Maniatis, T., and Green, M.A. (1984) Cell 38, 317–331.

    PubMed  CAS  Google Scholar 

  11. Konarska, M.M., Grabowski, P.J., Padgett, R.A., and Sharp, P.A. (1985) Nature 313, 552–557.

    PubMed  CAS  Google Scholar 

  12. Rodriguez, J.R., Pikielny, C.W., and Rosbash, M. (1984) Cell 39, 603–610.

    PubMed  CAS  Google Scholar 

  13. Domdey, H., Apostol, B., Lin, R.-J., Newman, A., Brody, E., and Abelson, J. (1984) Cell 39, 611–621.

    PubMed  CAS  Google Scholar 

  14. Zeitlin, S., and Efstratiadis, A. (1984) Cell 39, 589–602.

    PubMed  CAS  Google Scholar 

  15. Mount, S. (1982) Nucleic Acids Res. 10, 459–472.

    PubMed  CAS  Google Scholar 

  16. Breathnach, R., Benoist, C., O’Hare, K., Gannon, F., and Chambon, P. (1978) Proc. Natl. Acad. Sci. USA 75, 4853–4857.

    PubMed  CAS  Google Scholar 

  17. Mount, S., and Steitz, J. (1983) Nature 303, 380–381.

    PubMed  CAS  Google Scholar 

  18. Aebi, M., Hornig, H., Padgett, R.A., Reiser, J., and Weissmann, C. (1986) Cell 47, 555–565.

    PubMed  CAS  Google Scholar 

  19. Wieringa, B., Hofer, E., and Weissman, C. (1984) Cell 37, 915–925.

    PubMed  CAS  Google Scholar 

  20. Van Santen, V.L., and Spritz, R.A. (1985) Proc. Natl. Acad. Sci. USA 82, 2885–2889.

    PubMed  Google Scholar 

  21. Fu, X.-Y., Ge, H., and Manley, J.L. (1988) EMBO J. 7, 809–817.

    PubMed  CAS  Google Scholar 

  22. Reed, R., and Maniatis, T. (1985) Cell 41, 95–105.

    PubMed  CAS  Google Scholar 

  23. Ruskin, B., and Green, M.R. (1985) Nature 317, 732–734.

    PubMed  CAS  Google Scholar 

  24. Langford, C.J., Klinz, F.-J., Donath, C., and Gallwitz, D. (1984) Cell 36, 645–653.

    PubMed  CAS  Google Scholar 

  25. Teem, J.L., Abovich, N., Kaufer, N.F., Schwindinger, W.F., Warner, J.R., Levy, A., Woolford, J., Leer, R.J., Van Raamsdonk-Duin, N.N.C., Nager, W.H., Planta, R.J., Schultz, L., Friesen, J.D., Fried, H., and Roshbash, N. Nucleic Acids Res. 12, 8295–8312.

    Google Scholar 

  26. Gallwitz, D. (1982) Proc. Natl. Acad. Sci. USA 79, 3493–3497.

    PubMed  CAS  Google Scholar 

  27. Parker, R., and Guthrie, C. (1985) Cell 41, 107–118.

    PubMed  CAS  Google Scholar 

  28. Newman, A.J., Lin, R.-J., Cheng, S.-C., and Abelson, J. (1985) Cell 42, 335–344.

    PubMed  CAS  Google Scholar 

  29. Jacquier, A., and Roshbash, M. (1986) Proc. Natl. Acad. Sci. USA 83, 5835–5839.

    PubMed  CAS  Google Scholar 

  30. Vijayraghavan, V., Parker, R., Tamm, J., Iimura, Y., Rossi, J., Abelson, J., and Guthrie, C. (1986) EMBO J. 5, 1683–1695.

    PubMed  CAS  Google Scholar 

  31. Fouser, L.A., and Friesen, J.D. (1986) Cell 45, 81–93.

    PubMed  CAS  Google Scholar 

  32. Langford, C.J., and Gallwitz, D. (1983) Cell 33, 519–527.

    PubMed  CAS  Google Scholar 

  33. Fouser, L.A., and Friesen, J.D. (1987) Mol. Cell. Biol. 7, 225–230.

    PubMed  CAS  Google Scholar 

  34. Rymond, B.C., and Roshbash, M. (1985) Nature 317, 735–737.

    PubMed  CAS  Google Scholar 

  35. Noble, J.C.S., Pan, Z.-Q., Prives, C., and Manley, J.L. (1987) Cell 50, 227–236.

    PubMed  CAS  Google Scholar 

  36. Keller, E.B., and Noon, W.A. (1984) Proc. Natl. Acad. Sci. USA 81, 7417–7420.

    PubMed  CAS  Google Scholar 

  37. Hornig, H., Aebi, M., and Weissmann, C. (1986) Nature 324, 589–591.

    PubMed  CAS  Google Scholar 

  38. Padgett, R.A., Konarska, M.M., Aebi, M., Hronig, H., Weissmann, C., and Sharp, P.A. (1985) Proc. Natl. Acad. Sci. USA 82, 8349–8353.

    PubMed  CAS  Google Scholar 

  39. Ruskin, B., Green, J.M., and Green M.R. (1985) Cell 41, 833–834.

    PubMed  CAS  Google Scholar 

  40. Jacquier, A., Rodriguez, J.R., and Roshbash, M. (1985) Cell 43, 423–430.

    PubMed  CAS  Google Scholar 

  41. Cellini, A., Parker, R., McMahon, J., Guthrie, C., and Rossi, J. (1986) Mol. Cell. Biol. 6, 1571–1578.

    PubMed  CAS  Google Scholar 

  42. Pikielny, C.W., Teem, J.L., and Roshbash, M. (1983) Cell 34, 395–402.

    PubMed  CAS  Google Scholar 

  43. Cellini, A., Felder, E., and Rossi, J. (1986) EMBO J. 5, 1023–1030.

    PubMed  CAS  Google Scholar 

  44. Brietbart, R.E., Andreadis, A., and Nadal-Ginard, B. (1987) Annu. Rev. Biochem. 56, 467–495.

    Google Scholar 

  45. Birnstiel, M.L., ed. (1988). Structure and Function of Major and Minor Small Nuclear Ribonucleoprotein Particles. Springer-Verlag, Berlin, 216 pp.

    Google Scholar 

  46. Lerner, M.R., Boyle, J.A., Mount, S., Wolin, S.L., and Steitz, J.A. (1980) Nature 283, 220–224.

    PubMed  CAS  Google Scholar 

  47. Rogers, J., and Wall, R. (1980) Proc. Natl. Acad. Sci. USA 77, 1877–1879.

    PubMed  CAS  Google Scholar 

  48. Mount, S.M., Petterson, I., Hinterberger, M., Karmas, A., and Steitz, J.A. (1983) Cell 33, 509–518.

    PubMed  CAS  Google Scholar 

  49. Black, D.L., Chabot, B., and Steitz, J.A. (1985) Cell 42, 737–750.

    PubMed  CAS  Google Scholar 

  50. Chabot, B., and Steitz, J.A. (1987) Mol. Cell. Biol. 7, 281–293.

    PubMed  CAS  Google Scholar 

  51. Chabot, B., and Steitz, J.A. (1987) Mol. Cell. Biol. 7, 698–707.

    PubMed  CAS  Google Scholar 

  52. Kramer, A., Keller, W., Appel, B., and Luhrmann, R. (1984) Cell 38, 299–307.

    PubMed  CAS  Google Scholar 

  53. Fradin, A., Jove, R., Hemenway, C., Keiser, H.D., Manley, J.L., and Prives, C. (1984) Cell 37, 927–936.

    PubMed  CAS  Google Scholar 

  54. Padgett, R.A., Mount, S.M., Steitz, J.A., and Sharp, P.A. (1983) Cell 35, 101–107.

    PubMed  CAS  Google Scholar 

  55. Zhuang, Y., and Weiner, A.M. (1986) Cell 46, 827–835.

    PubMed  CAS  Google Scholar 

  56. Ruskin, B., and Green, M.R. (1985) Cell 43, 131–142.

    PubMed  CAS  Google Scholar 

  57. Zillman, M., Rose, S.D., and Berget, S.M. (1987) Mol. Cell. Biol. 7, 2877–2883.

    Google Scholar 

  58. Ares, M. (1986) Cell 47, 49–59.

    PubMed  CAS  Google Scholar 

  59. Ruskin, B., Zamore, P.D., and Green, M.R. (1988) Cell 52, 207–219.

    PubMed  CAS  Google Scholar 

  60. Tazi, J., Alibert, C., Temsamani, J., Reveillaud, I., Cathala, G., Burnel, C., and Jeanteur, P. (1986) Cell 47, 755–766.

    PubMed  CAS  Google Scholar 

  61. Gerke, V., and Steitz, J.A. (1986) Cell 47, 973–984.

    PubMed  CAS  Google Scholar 

  62. Parker, R., Siliciano, P.G., and Guthrie, C. (1987) Cell 49, 229–239.

    PubMed  CAS  Google Scholar 

  63. Van der Veen, R., Arnberg, A.C., Van der Horst, G., Bonen, L., Tabak, H.F., and Grivell, L.A. (1986) Cell 44, 225–234.

    PubMed  Google Scholar 

  64. Schmelzer, C., and Muller, M.W. (1987) Cell 51, 753–762.

    PubMed  CAS  Google Scholar 

  65. Van der Veen, R., Kwakman, J.H., and Grivell, L.A. (1987) EMBO J. 6, 3827–3831.

    PubMed  Google Scholar 

  66. Couto, J.R., Tamm, J., Parker, R., and Guthrie, C. (1987) Genes Devel. 1, 445–455.

    PubMed  CAS  Google Scholar 

  67. Pikielny, C.W., Rymond, B.C., and Roshbash, M. (1986) Nature 324, 341–345.

    PubMed  CAS  Google Scholar 

  68. Chabot, B., Black, D.L., LeMaster, D.M., and Steitz, J.A. (1985) Science 230, 1344–1349.

    PubMed  CAS  Google Scholar 

  69. Berget, S.M., and Robberson, B.L. (1986) Cell 46, 691–696.

    PubMed  CAS  Google Scholar 

  70. Black, D.L., and Steitz, J.A. (1986) Cell 46, 697–704.

    PubMed  CAS  Google Scholar 

  71. Kretzner, L., Rymond, B.C., and Roshbash, M. (1987) Cell 50, 593–602.

    PubMed  CAS  Google Scholar 

  72. Siliciano, P.G., Brow, D.A., Roiba, H., and Guthrie, C. (1987) Cell 50, 585–592.

    PubMed  CAS  Google Scholar 

  73. Patterson, B., and Guthrie, C. (1987) Cell 49, 613–624.

    PubMed  CAS  Google Scholar 

  74. Grabowski, P.J., Seiler, S.R., and Sharp, P.A. (1985) Cell 42, 345–353.

    PubMed  CAS  Google Scholar 

  75. Frendeway, D., and Keller, W. (1985) Cell 42, 355–367.

    Google Scholar 

  76. Bindereif, A., and Green, M.R. (1986) Mol. Cell. Biol. 6, 2582–2592.

    PubMed  CAS  Google Scholar 

  77. Brody, E., and Abelson, J. (1985) Science 228, 963–967.

    PubMed  CAS  Google Scholar 

  78. Bindereif, A., and Green, M.R. (1987) EMBO J. 6, 2415–2424.

    PubMed  CAS  Google Scholar 

  79. Konarska, M.M., and Sharp, P.A. (1987) Cell 49, 763–774.

    PubMed  CAS  Google Scholar 

  80. Lamond, A.I., Konaraka, M.M., Grabowski, P.J., and Sharp, P.A. (1988) Proc. Natl. Acad. Sci. USA 85, 411–415.

    PubMed  CAS  Google Scholar 

  81. Cheng, S.-C., and Abelson, J. (1987) Genes Devel. 1, 1014–1027.

    PubMed  CAS  Google Scholar 

  82. Grabowski, P.J., and Sharp, P.A. (1985) Science 223, 1294–1299.

    Google Scholar 

  83. Konarska, M.M., and Sharp, P.A. (1985) Cell 48, 845–855.

    Google Scholar 

  84. Lamond, A.I., Konarska, M.M., and Sharp, P.A. (1987) Genes Devel. 1, 532–543.

    PubMed  CAS  Google Scholar 

  85. Breitbart, R.E., and Nadal-Ginard, B. (1987) Cell 49, 793–803.

    PubMed  CAS  Google Scholar 

  86. Noble, J.C.S., Prives, C., and Manley, J.L. (1986) Nucleic Acids. Res. 14, 1219–1235.

    PubMed  CAS  Google Scholar 

  87. Schmitt, P., Gattoni, R., Keohavong, P., and Stevenin, J. (1987) Cell 50, 31–39.

    PubMed  CAS  Google Scholar 

  88. Eperon, L.P., Estibeivo, J.P., and Eperon, I.C. (1986) Nature 324, 280–282.

    PubMed  CAS  Google Scholar 

  89. Zhuang, Y., Leung, H., and Weiner, A.M. (1987) Mol. Cell. Biol. 7, 3018–3020.

    PubMed  CAS  Google Scholar 

  90. Fu, X.-Y., Colgan, J., and Manley, J.L. (1988) Mol. Cell. Biol. 8, 3582–3590.

    PubMed  CAS  Google Scholar 

  91. Falkendahl, S., Parker, V.P., and Davidson, N. (1985) Proc. Natl. Acad. Sci. USA 82, 449–453.

    Google Scholar 

  92. Rozek, C.E., and Davidson, N. (1986) Proc. Natl. Acad. Sci. USA 82, 2128–2132.

    Google Scholar 

  93. Bernstein, S.I., Hansen, C.J., Becker, K.D., Wassenberg, D.R., Roche, E.S., Donady, J.J., and Emerson, C.P. (1986) Mol. Cell. Biol. 6, 2511–2519.

    PubMed  CAS  Google Scholar 

  94. Ulfendahl, P.J., Pettersson, U., and Akusjarvi, G. (1985) Nucleic Acids Res. 13, 6299–6315.

    PubMed  CAS  Google Scholar 

  95. Fu, X.-Y., and Manley, J.L. (1987) Mol. Cell. Biol. 7, 738–748.

    PubMed  CAS  Google Scholar 

  96. Manley, J.L., Fu, X.-Y., Noble, J.C.S., and Ge, H. (1987) In Molecular Biology of RNA: New Perspectives. Inouye, M., and Dudock, B.S. (eds.). Academic Press, New York, pp. 97–112.

    Google Scholar 

  97. Noble, J.C.S., Prives, C., and Manley, J.L. (1988) Genes Devel. 2, 1460–1475.

    PubMed  CAS  Google Scholar 

  98. Noble, J.C.S., Ge, H., Chaudhuri, M., and Manley, J.L. (1989) Mol. Cell. Biol. 9, in press.

    Google Scholar 

  99. Treisman, R., Cowie, A., Favaloro, J., Jat, P., and Kamen, R. (1981) J. Mol. Appl. Genet. 1, 83–92.

    PubMed  CAS  Google Scholar 

  100. Khoury, G., Gruss, P., Dhar, R., and Lai, C.-J. (1979) Cell 18, 85–92.

    PubMed  CAS  Google Scholar 

  101. Piatak, M., Subramanian, K.N., Roy, P., and Weissmann, S.M. (1981) J. Mol. Biol. 153, 589–618.

    PubMed  CAS  Google Scholar 

  102. Somasekhar, M.B., and Mertz, J.E. (1985) Nucleic Acids. Res. 13, 5591–5609.

    PubMed  CAS  Google Scholar 

  103. Plotch, S.J., and Krug, R.M. (1986) Proc. Natl. Acad. Sci. USA 83, 5444–5448.

    PubMed  CAS  Google Scholar 

  104. Solnick, D. (1985) Cell 43, 667–676.

    PubMed  CAS  Google Scholar 

  105. Solnick, D., and Lee, S.I. (1987) Mol. Cell. Biol. 7, 3194–3198.

    PubMed  CAS  Google Scholar 

  106. Kuhne, T., Wieringa, B., Reiser, J., and Weissman, C. (1983) EMBO J. 2, 727–733.

    PubMed  CAS  Google Scholar 

  107. Lang, K.M., and Spritz, R.A. (1983) Science 220, 1351–1355.

    PubMed  CAS  Google Scholar 

  108. Reed, R., and Maniatis, T. (1986) Cell 46, 681–690.

    PubMed  CAS  Google Scholar 

  109. Lund, E., and Dahlberg, J.E. (1987) Genes Devel. 1, 39–46.

    PubMed  CAS  Google Scholar 

  110. Lund, E., Kahan, B., and Dahlberg, J.E. (1985) Science 229, 1271–1274.

    PubMed  CAS  Google Scholar 

Download references

Authors
  1. Jonathan C. S. Noble
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. James L. Manley
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Department of Biochemistry, University of Minnesota Medical School, Minneapolis, MN, 55455, USA

    Kenneth W. Adolph

Rights and permissions

Reprints and permissions

Copyright information

© 1989 Springer-Verlag New York Inc.

About this chapter

Cite this chapter

Noble, J.C.S., Manley, J.L. (1989). The Mechanism and Control of Pre-mRNA Splicing. In: Adolph, K.W. (eds) Molecular Biology of Chromosome Function. Springer, New York, NY. https://doi.org/10.1007/978-1-4612-3652-8_11

Download citation

  • DOI: https://doi.org/10.1007/978-1-4612-3652-8_11

  • Publisher Name: Springer, New York, NY

  • Print ISBN: 978-1-4612-8192-4

  • Online ISBN: 978-1-4612-3652-8

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation