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Enzymes of DNA Precursor Synthesis and the Control of DNA Replication

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Molecular Biology of Chromosome Function

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Abstract

Although the enzymes of DNA precursor biosynthesis were originally described in the 1950s and 1960s, it is still timely to discuss these proteins with respect to their involvement in regulating DNA replication. This chapter will focus on current research that emphasizes the genetics and cell biology of these enzymes: How do the enzymes interact in cells, with each other, and with replication proteins? What controls reaction fluxes through individual enzymes in vivo? How do these fluxes vary through the cell cycle, and what factors control the variations? What are the effective concentrations of deoxyribonucleoside triphosphates (dNTPs*) at replication sites? How do variations in dNTP pools affect the rate and accuracy of DNA replication? What are the metabolic and genetic consequences of inhibiting a particular enzyme with an antimetabolite? Metabolic inhibitors provide useful probes for understanding compartmentation and control. In turn, this understanding could lead to the more effective use of antimetabolites as anticancer, antimicrobial, or antiviral agents.

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Abbreviations

rNMP, rNDP, rNTP:

ribonucleoside mono-, di-, and triphosphate, respectively

dNMP, dNDP, dNTP:

deoxyribonucleoside mono-, di-, and triphosphate, respectively

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Authors
  1. Christopher K. Mathews
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Editor information

Editors and Affiliations

  1. Department of Biochemistry, University of Minnesota Medical School, Minneapolis, MN, 55455, USA

    Kenneth W. Adolph

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© 1989 Springer-Verlag New York Inc.

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Mathews, C.K. (1989). Enzymes of DNA Precursor Synthesis and the Control of DNA Replication. In: Adolph, K.W. (eds) Molecular Biology of Chromosome Function. Springer, New York, NY. https://doi.org/10.1007/978-1-4612-3652-8_1

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  • DOI: https://doi.org/10.1007/978-1-4612-3652-8_1

  • Publisher Name: Springer, New York, NY

  • Print ISBN: 978-1-4612-8192-4

  • Online ISBN: 978-1-4612-3652-8

  • eBook Packages: Springer Book Archive

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