Binding Patterns of the Monoclonal Antibodies in the 10th Workshop 2-D Gel Analysis of Class II Antigens

Abstract

The monoclonal antibodies used for the Workshop two-dimensional (2-D) gel study were selected to satisfy several criteria. In order to use them for the 2-D gel study and the study of alloreactive T-cell clones, mAbs with demonstrated ability to immunoprecipitate class II molecules and block alloreactive T-cell clones were given highest priority. Additional antibodies were selected that had unique polymorphic specificities or unique α or β chain specificity. A listing of antibodies with the laboratory of origin is given in Table 1. Their reported specificity at the time of submission is indicated, but in many cases the results of serologic and biochemical analysis during this Workshop 2-D gel study suggested that they are broader or more limited than was expected (as indicated by the asterisk). Additional polymorphic antibodies were submitted, but these preparations were found to have weak reactivity in the serologic screening and were not analyzed further. In order to set the priority for choosing mAbs to use for immunoprecipitation in the 2-D gel study, they were examined for binding to the Workshop core cell lines using a quantitative indirect immunofluorescence assay.

Participating Laboratories: US7KN0¹, US7NFL²