Advertisement

Segregation Analysis of HLA Class I Determinants by Biochemistry

  • Gabriele Frenz
  • Ilias Doxiadis
  • Udo Vögeler
  • Gabriele Doxiadis
  • Hans Grosse-Wilde
Conference paper

Abstract

To date, the HLA class I alloantigenic determinants have been defined by classic serology. Methods such as one dimensional isoelectric focusing (1D-IEF) were used predominantly for the biochemical definition of these antigens from B-lymphoblastoid cell lines (B-LCL) by immunoprecipitation with respective monoclonal anti bodies (1). The data defined HLA-A,B products by comparative analyses. Non-A and non-B locus products have not yet been identified by gel electrophoresis.

Keywords

Segregation Analysis Anti Body Neuraminidase Treatment Respective Antigen Chain Serum 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. 1.
    Yang SY, Morishima Y, Collins NY, et al. Comparison of one-dimensional IEF patterns for serologically detectable HLA-A and B allotypes. Immunogenetics 1984; 19:217.PubMedCrossRefGoogle Scholar
  2. 2.
    Neefjes JJ, Doxiadis I, Stam NJ, Beckers CJ, Ploegh HL. An analysis of class I antigens of man and other species by one- dimensional IEF and immunoblotting. Immunogenetics 1986;23:164.PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag New York 1989

Authors and Affiliations

  • Gabriele Frenz
  • Ilias Doxiadis
  • Udo Vögeler
  • Gabriele Doxiadis
  • Hans Grosse-Wilde

There are no affiliations available

Personalised recommendations