To date, the HLA class I alloantigenic determinants have been defined by classic serology. Methods such as one dimensional isoelectric focusing (1D-IEF) were used predominantly for the biochemical definition of these antigens from B-lymphoblastoid cell lines (B-LCL) by immunoprecipitation with respective monoclonal anti bodies (1). The data defined HLA-A,B products by comparative analyses. Non-A and non-B locus products have not yet been identified by gel electrophoresis.
Segregation Analysis Anti Body Neuraminidase Treatment Respective Antigen Chain Serum
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Yang SY, Morishima Y, Collins NY, et al. Comparison of one-dimensional IEF patterns for serologically detectable HLA-A and B allotypes. Immunogenetics 1984; 19:217.PubMedCrossRefGoogle Scholar
Neefjes JJ, Doxiadis I, Stam NJ, Beckers CJ, Ploegh HL. An analysis of class I antigens of man and other species by one- dimensional IEF and immunoblotting. Immunogenetics 1986;23:164.PubMedCrossRefGoogle Scholar