Segregation Analysis of HLA Class I Determinants by Biochemistry

  • Gabriele Frenz
  • Ilias Doxiadis
  • Udo Vögeler
  • Gabriele Doxiadis
  • Hans Grosse-Wilde
Conference paper

Abstract

To date, the HLA class I alloantigenic determinants have been defined by classic serology. Methods such as one dimensional isoelectric focusing (1D-IEF) were used predominantly for the biochemical definition of these antigens from B-lymphoblastoid cell lines (B-LCL) by immunoprecipitation with respective monoclonal anti bodies (1). The data defined HLA-A,B products by comparative analyses. Non-A and non-B locus products have not yet been identified by gel electrophoresis.

Keywords

Electrophoresis 

References

  1. 1.
    Yang SY, Morishima Y, Collins NY, et al. Comparison of one-dimensional IEF patterns for serologically detectable HLA-A and B allotypes. Immunogenetics 1984; 19:217.PubMedCrossRefGoogle Scholar
  2. 2.
    Neefjes JJ, Doxiadis I, Stam NJ, Beckers CJ, Ploegh HL. An analysis of class I antigens of man and other species by one- dimensional IEF and immunoblotting. Immunogenetics 1986;23:164.PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag New York 1989

Authors and Affiliations

  • Gabriele Frenz
  • Ilias Doxiadis
  • Udo Vögeler
  • Gabriele Doxiadis
  • Hans Grosse-Wilde

There are no affiliations available

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