Abstract
One-dimensional isoelectric focusing (1D-IEF) gel electrophoresis was used for the study of class I HLA molecules in the Class I HLA Biochemistry component of the Workshop. The method used is a modified version of the two-dimensional gel electrophoresis technique originally developed by O’Farrell (1). The Workshop technique utilized a vertical slab gel apparatus and IEF samples obtained from metabolically labeled cell lysates by immunoprecipitation using monoclonal antibodies. The immunoprecipitated HLA antigens are desialated by neuraminidase treatment to obtain a single, strongly expressed IEF band for each antigen.
Participating Laboratory: US7SYY1
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References
O’Farrell PH. High resolution two-dimensional electrophoresis of proteins. J Biol Chem 1975; 250: 4007.
Yang SY, Morishima Y, Collins NH, et al. Comparison of one dimensional IEF patterns for serologically detectable HLA-A and HLA-B allotypes. Immunogenetics 1984; 19: 217.
Parham P, Barnstable CJ, Bodmer WF. Use of a monoclonal antibody (w6/32) in structural studies of HLA-A, B, C antigens. J Immunol 1979; 123: 324.
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© 1989 Springer-Verlag New York
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Yang, S.Y. (1989). A Standardized Method for Detection of HLA-A and HLA-B Alleles by One-Dimensional Isoelectric Focusing (IEF) Gel Electrophoresis. In: Dupont, B. (eds) Immunobiology of HLA. Springer, New York, NY. https://doi.org/10.1007/978-1-4612-3552-1_66
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DOI: https://doi.org/10.1007/978-1-4612-3552-1_66
Publisher Name: Springer, New York, NY
Print ISBN: 978-1-4612-8154-2
Online ISBN: 978-1-4612-3552-1
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