HLA-Dw and HLA-DP Typing of the Reference Panel of B-Lymphoblastoid Cell Lines

Abstract

Cellular typing for HLA-Dw and HLA-DPw within the Tenth International Histocompatibility Workshop was incorporated into the Workshop component on T-cell Recognition of HLA Class II Molecules. Separate Workshop components on Dw and DPw typing for identification of new specificities using homozygous typing cells (HTC) or primed lymphocyte typing (PLT) were not designed as part of the Tenth Workshop. It was, however, essential that accurate information was obtained for HLA-Dw and HLA-DPw specificities for the Reference Panel of B- Lymphoblastoid Cell Lines. A working group consisting of the authors of this paper was established for this purpose. Whenever possible, any residual PBL from the cell line donors was collected and distributed to several interested laboratories for cellular typing studies. In the case of DP, typing was also performed using the B-cell lines themselves. DP typing was performed by four groups: Sell and Eckels, Hartzman and Robbins, Ødum and Svejgaard, and Farrell and Honeyman. The results of the DP typing studies are shown in Table 1. Consensus assignments of HLA-Dw and HLA-DPw specificities are incorporated into Table 4B in the paper entitled “Description of the Reference Panel of B-Lymphoblastoid Cell Lines for Factors of the HLA System” by Yang et al. (this volume).

Participating Laboratory: US7HAN,¹ US2BAC,² USIHJH,³ SCASV,⁴ ANZBSH,⁵ US7NFL,⁶ US2MBC,⁷