RFLP Standardization Report for DR Beta/SstI

Abstract

The DR beta/SstI system identified the 28 fragments as shown in Figure 1 and Table 1. Some close fragments from St. fragment no. 8 to no. 14 were difficult to analyze even in the long migration condition. St. fragment no. 4, no. 22, and no. 25 were found only in a single laboratory. Two fragments (no. 22 and no. 25) are DQ-dominant. Cross-hybridizing bands are shown in Table 2. Of the 28 fragments, 18 are dominantly DR-specific. St. fragment no. 16 and no. 17 are DR-DO codominant. Six fragments are DQ-dominant and four are DP- dominant. Fine bands (St. fragment no. 1, no. 6, no. 7, no. 14, and no. 28) showed no cross-hybridization with other beta probes. The fragments absence/presence data on each cell line with the DRβ probe are shown in Table 3. St. fragment no. 12 (11.59 kb) and no. 16 (6.15kb) are highly correlated with DR1 and DRw15, respectively. St. fragment no. 2 (21.12kb), no. 14 (10.95kb), and no. 17 (5.19kb) are shown the common banding pattern in each of the 72 core cell lines. Twenty-two cell lines showed these three bands, including all but one of the DRw53 cells.

Participating Laboratories: JAPTSU,¹ JAPSAS,² BENREE³