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Immunodiagnostic Approaches to the Detection of M. leprae Infection in Leprosy

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Progress in Vaccinology

Part of the book series: Progress in Vaccinology ((VACCINOLOGY,volume 2))

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Abstract

Clinical symptoms aided by smear and histological examination are the conventional parameters used for the diagnosis of leprosy. These criteria, however, have self-imposed limitations, and it is sometimes difficult to diagnose the very early stage of the disease. Similarly, it is difficult to determine inactivity in some cases. Another important problem associated with epidemiologic studies of leprosy is the lack of reliable information on the occurrence of subclinical infection in leprosy, with the result that these studies are based mainly on the occurrence of manifest disease. There is sufficient evidence indicating that in leprosy, as in tuberculosis, there are far more persons with subclinical infection in any given area than those with overt disease. It is for this reason that the detection of subclinical leprosy infections are of paramount importance. Such detection may be useful for establishing the extent of infection and the course of the disease, as well as instituting proper immuno- or chemoprophylaxis.

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References

  1. Abe M: Specific serodiagnostic tests for leprosy, in Chatterjee BR (ed): Window on Leprosy. Wardha, India, Gandhi Memorial Leprosy Foundation, 1978, pp 235–241.

    Google Scholar 

  2. Abe M, Izumi S, Saito T, Mathur SK: Early serodiagnosis of leprosy by indirect immunofluorescence. Lepr India 1976; 48: 272–276

    PubMed  CAS  Google Scholar 

  3. Abe M, Minagawa F, Yoshino Y, et al: Fluorescent antibody absorption (FLA- ABS) test for detecting subclinical infection with Mycobacterium leprae. Int J Lepr 1980; 48: 109–119.

    CAS  Google Scholar 

  4. Abe M, Yoshino Y: Antigenic specificity of M. leprae by indirect immunofluorescence. Int J Lepr 1978; 46: 119.

    Google Scholar 

  5. Atlaw T, Kozbor D, Roder JC: Human monoclonal antibodies against Mycobacterium leprae. Infect Immun 1985; 49: 104–110.

    PubMed  CAS  Google Scholar 

  6. Bharadwaj VP: Immunodiagnostic approaches to the early detection of subclinical infection in leprosy. Ann Natl Acad Med Sei (India) 1985; 21: 128–139.

    Google Scholar 

  7. Bharadwaj VP, Desikan KV: Fluorescent leprosy antibody absorption (FLA-ABS) test for early serodiagnosis of leprosy. Int J Lepr 1979; 47 (suppl): 391.

    Google Scholar 

  8. Bharadwaj VP, Ramu G, Desikan KV: Fluorescent leprosy antibody absorption (FLA-ABS) test for early serodiagnosis of leprosy. Lepr India 1981; 53: 518–524.

    PubMed  CAS  Google Scholar 

  9. Bharadwaj VP, Ramu G, Desikan KV: A preliminary report on subclinical infection in leprosy. Lepr India 1982; 54: 220–227.

    PubMed  CAS  Google Scholar 

  10. Bharadwaj VP, Ramu G, Desikan KV: Immunoepidemiological studies on subclinical infection in leprosy in the household contacts in India—a preliminary report. Working paper for the 7th Joint US-Japan Conference on Leprosy Research. Sendai, Japan, 25–27 August 1982.

    Google Scholar 

  11. Bharadwaj VP, Ramu G, Desikan KV, Katoch K: Studies on subclinical in¬fection in leprosy, in Proceedings of the Workshop on Serological Tests for Detecting Sub-clinical Infection in Leprosy. Tokyo, Sasakawa Memorial Health Foundation, 1983, pp 29–35.

    Google Scholar 

  12. Bharadwaj VP, Ramu G, Desikan KV, Katoch K: Extended studies on subclinical infection in leprosy. Indian J Lepr 1984; 56: 807–812.

    PubMed  CAS  Google Scholar 

  13. Bharadwaj VP, Katoch K, Ramu G, et al: Seroepidemiological studies in subsided cases of multi-and paucibacillary types of leprosy using FLA-ABS test. Indian J Lepr 1987; 59: 30–35.

    PubMed  CAS  Google Scholar 

  14. Brett SJ, Draper P, Payne SN, Rees RJW: Serological activity of a characteristic phenolic glycolipid from Mycobacterium leprae in sera from patients with leprosy and tuberculosis. Clin Exp Immunol 1983; 52: 271–279.

    PubMed  CAS  Google Scholar 

  15. Caldwell HD, Kirchheimer WF, Buchanan FM: Identification of Mycobacterium leprae specific protein antigen and its possible application for Serodiagnosis of leprosy. Int J Lepr 1979; 47: 477–488.

    CAS  Google Scholar 

  16. Chatteijee BR: Is early diagnosis of leprosy in an Indian village not possible? Lepr India 1973; 45: 225–227.

    Google Scholar 

  17. Chatteijee BR, Thomas J, Taylor CE, Naidu GN: Epidemiological findings of a longitudinal study in a defined population in Jhalda, West Bengal, in Pro¬ceedings of All India Leprosy Workers Conference: Silver Jubilee and Centenary of Hansen’s Bacillus Discovery, Sevagram, India, abstract, 1973, p 5.

    Google Scholar 

  18. Cho SN, Yanagihara DL, Hunter SW, et al: Serological specificity of phenolic glycolipid I from Mycobacterium leprae and use in serodiagnosis of leprosy. Infect Immun 1983; 41: 1077–1083.

    PubMed  CAS  Google Scholar 

  19. Dharmendra: Detection of sub-clinical infection in leprosy (editorial). Lepr India 1982; 54: 193–203.

    PubMed  CAS  Google Scholar 

  20. Dharmendra: Lepromin test, in Dharmendra (ed): Leprosy, Vol 2. Bombay, Samant, 1985, pp 999–1066.

    Google Scholar 

  21. Dharmendra: Immuno-epidemiological surveys for detection of subclinical infection, in Dharmendra (ed): Leprosy, Vol 2. Bombay, Samant, 1985, pp 1207–1222.

    Google Scholar 

  22. Douglas JT, Naka SO, Lee JW: Development of an ELISA for detection of antibody in leprosy. Int J Lepr 1984; 52: 19–25.

    CAS  Google Scholar 

  23. Douglas JT, Worth RM: Field evaluation of an ELISA test to detect antibody in leprosy patients and their contacts. Int J Lepr 1984; 52: 26–33.

    CAS  Google Scholar 

  24. Douglas JT, Worth RM, Murray CJ, et al: ELISA techniques with application to leprosy, in Proceedings of the Workshop on Serological Tests for Detecting Sub-clinical Infection in Leprosy. Tokyo, Sasakawa Memorial Health- Foundation, 1983, pp 85–90.

    Google Scholar 

  25. Figuerdo N, Desai SD: Positive bacillary findings in the skin of contacts of leprosy patients. Indian J Med Sci 1949; 4: 253–262.

    Google Scholar 

  26. Gillis TP, Buchanan TM: Production and partial characterisation of monoclonal antibodies to Mycobacterium leprae. Infect Immun 1982; 37: 172–178.

    PubMed  CAS  Google Scholar 

  27. Godal T: Immunological detection of sub-clinical infection in leprosy. Lepr India 1975; 47: 30–41.

    Google Scholar 

  28. Godal T, Negassi K: Subclinical infection in leprosy. Br Med J. 1973; 2: 557–560.

    Article  Google Scholar 

  29. Harboe M, Closs O, Bjorvatn B, et al: Antibody responses in rabbits to immunization with Mycobacterium leprae. Infect Immun 1977; 18: 792–805.

    PubMed  CAS  Google Scholar 

  30. Harboe M, Closs O, Bjune G, et al: Mycobacterium leprae specific antibodies detected by radio-immunoassay. Scand J Immunol 1978; 7: 11–120.

    Article  Google Scholar 

  31. Hunter SW, Brennan PJ: A novel phenolic glycolipid from Mycobacterium leprae possibly involved in immunogenicity and pathogenicity J Bacteriol 1981; 147: 728–735.

    PubMed  CAS  Google Scholar 

  32. Ivanyi J, Sinha S, Aston R, et al: Definition of species specific and cross reacting antigenic determinants of M. leprae using monoclonal antibodies. Clin Exp Immunol 1983; 52: 528–536.

    PubMed  CAS  Google Scholar 

  33. Jagannath C, Sengupta DN: Serology of leprosy. I. Indirect haemagglutination test with stabilised, sensitized red cells. Lepr India 1981; 53: 507–512.

    PubMed  CAS  Google Scholar 

  34. Kim Do II, Lee H-Y: Some experimental studies on the fluorescent leprosy antibody absorption (FLA-ABS) tests for leprosy patients and household contacts, in Proceedings of the Workshop on Serological Tests for Detecting Subclinical Infection in Leprosy. Tokyo., Sasakawa Memorial Health Foundation, 1983, pp 49–54.

    Google Scholar 

  35. Kronvall G, Stanford JL, Walsch GP: Studies of mycobacterial antigens with special reference to Mycobacterium leprae. Infect Immun 1976; 13: 1132–1139.

    PubMed  CAS  Google Scholar 

  36. Kumar S, Moudgill KD, Band AH, et al: A dot enzyme immunoassay for detection of IgM antibodies against phenolic glycolipid-I in sera from leprosy patients. Indian J Lepr 1986; 58: 185–190.

    PubMed  CAS  Google Scholar 

  37. Melsom R, Harboe M, Duncan ME, Bergsiwick M: IgA and IgM antibodies against Mycobacterium leprae in the cord sera and in patients with leprosy: an indicator of intrauterine infection in leprosy. Scand J Immunol 1981; 14: 342–352.

    Article  Google Scholar 

  38. Melsom R, Harboe M, Duncan ME: IgA, IgM and IgG anti M. leprae antibodies in babies of leprosy mothers during the first two years of life. Clin Exp Immunol 1982: 49: 532–542.

    PubMed  CAS  Google Scholar 

  39. Melsom R, Harboe M, Myrvang B, et al: Immunoglobulin class specific an¬tibodies to M. leprae in leprosy patients, including the indeterminate group and healthy contacts as a step in the development of methods for serodiagnosis of leprosy. Clin Exp Immunol 1982; 47: 225–233.

    PubMed  CAS  Google Scholar 

  40. Melsom R, Harboe M, Naafs B: Class specific anti-M. leprae antibodies assay in lepromatous (BL-LL) leprosy patients, during the first two to four years of DDS treatment. Int J Lepr 1982; 50: 271–281.

    CAS  Google Scholar 

  41. Melsom R, Naafs B, Harboe M, Closs O: Antibody activity against Mycobacterium leprae antigen 7 during the first year of DDS treatment in lepromatous (BL-LL) leprosy. Lepr Rev 1978; 49: 17–29.

    PubMed  CAS  Google Scholar 

  42. Petchelai B, Khupulsup KS, Sampattavanich S, et al: Serodiagnosis of leprosy by an indirect haemagglutination test, in Proceedings of the Workshop on Serological Tests for Detecting Subclinical Infection in Leprosy. Tokyo, Sasakawa Memorial Health Foundation, 1983, pp 77–78.

    Google Scholar 

  43. Qinixue WU, Ganyun YE, Xinyu L, et al: A preliminary study on serological activity of a phenolic glycolipid from Mycobacterium leprae in sera from patients with leprosy, tuberculosis and normal controls. Lepr Rev 1986; 57: 129–136.

    Google Scholar 

  44. Ralhan R, Band AH, Roy A, et al: An enzyme immunoassay titrating IgM antibody against phenolic glycolipid for diagnosis of lepromatous leprosy. Indian J Med Res 1985; 82: 110–115.

    PubMed  CAS  Google Scholar 

  45. Ramu G, Bharadwaj VP, Katoch K, Desikan KV: Studies on the healthy contacts of leprosy patients, in Proceedings of the Workshop on Serological Tests for Detecting Subclinical Infection in Leprosy. Tokyo, Sasakawa Memorial Health Foundation, 1983, pp 37–43.

    Google Scholar 

  46. Rees RJW: The significance of lepromin reaction in man. Prog Allergy 1964; 8: 224–237.

    Google Scholar 

  47. Reggiardo Z, Vazquez E, Schnaper L: ELISA tests for antibodies against mycobacterial glycolipids. J Immunol Methods 1980; 34: 55–60.

    Article  PubMed  CAS  Google Scholar 

  48. Rojas Espinosa O, Estrada-Para S, Serranomirado SA, Latapi E: Antimy- cobacterial antibodies in diffused lepromatous leprosy detected by counter- immunoelectrophoresis. Int J Lepr 1976; 44: 448–452.

    CAS  Google Scholar 

  49. Samuel NM, Adiga RB: Detection of antibodies in the sera of leprosy patients and contacts by immunoabsorbent assay (ELISA), in Proceedings of the Workshop on Serological Tests for Detecting Subclinical Infection in Leprosy. Tokyo, Sasakawa Memorial Health Foundation, 1983, pp 55–68.

    Google Scholar 

  50. Shepard CC, Saitz EW: Lepromin and tuberculin reactivity in adults not exposed to leprosy. J Immunol 1967; 99: 637–645.

    PubMed  CAS  Google Scholar 

  51. Sinha S, Sengupta U, Ramu G, Ivanyi J: A serological test for leprosy based on competitive inhibition of monoclonal antibody binding to MY2a determinant of M. leprae. Trans R Soc Trop Med Hyg 1983; 77: 869–871.

    Article  PubMed  CAS  Google Scholar 

  52. Sinha S, Sengupta U, Ramu G, Ivanyi J: Serological survey of leprosy and control subjects by a monoclonal antibody based immunoassay. Int J Lepr 1985; 53: 33–38.

    CAS  Google Scholar 

  53. Stanford JL, Rook GAW, Convit J, et al: Preliminary taxonomic studies on the leprosy bacillus. Br J Exp Pathol 1975; 56: 570–585.

    Google Scholar 

  54. Taylor CE, Elliston EP, Gideon H: Asymptomatic infection in leprosy. Int J Lepr 1965; 33: 716–731.

    PubMed  Google Scholar 

  55. Vithayasai V, Songsiri S, Vithayasai P, Nelson K: Serological study of leprosy by enzyme linked immunoabsorbent assay, in Proceedings of the Workshop on Serological Tests for Detecting Subclinical Infection in Leprosy. Tokyo, Sasakawa Memorial Health Foundation, 1983, pp 79–86.

    Google Scholar 

  56. Waters MFR: Significance of the lepromin test in tuberculin negative volunteers permanently resident in a leprosy free area. Presented at the Tenth International Leproy Congress, Bergen, 1973, p 81 (abstract).

    Google Scholar 

  57. Yoder L, Naffs B, Harboe M, Bjune G: Antibody activity against Mycobacterium leprae antigens in leprosy: studies on the variation of antibody content throughout the spectrum and the effect of DDS treatment and relapse in BT leprosy. Lepr Rev 1979; 50: 113–121.

    PubMed  CAS  Google Scholar 

  58. Young DB, Buchanan TM: The phenolic glycolipid from Mycobacterium leprae: use in serological tests, in Proceedings of the Workshop on Serological Tests for Detecting Subclinical Infection in Leprosy. Tokyo, Sasakawa Memorial Health Foundation, 1983; pp 19–24.

    Google Scholar 

  59. Young DB, Buchanan TM: A serological test for leprosy using a glycolipid specific for Mycobacterium leprae. Science 1983; 221: 1057–1059.

    Article  PubMed  CAS  Google Scholar 

  60. Young DB, Fohn MJ, Khanolkar SR, Buchanan TM: A spot test for detection of antibodies to phenolic glycolipid I. Lepr Rev 1985; 56: 193–198.

    PubMed  CAS  Google Scholar 

  61. Young DB, Khanolkar SR, Barg LL, Buchanan TM: Generation and characterisation of monoclonal antibodies to the phenolic glycolipid of Mycobacterium leprae. Infect Immun 1984; 43: 183–188.

    PubMed  CAS  Google Scholar 

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Authors
  1. V. P. Bharadwaj
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  2. Kiran Katoch
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Editor information

Editors and Affiliations

  1. National Institute of Immunology, JNU Complex, Shahid Jeet Singh Marg, 110 067, New Delhi, India

    G. P. Talwar

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© 1989 Springer-Verlag New York Inc.

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Bharadwaj, V.P., Katoch, K. (1989). Immunodiagnostic Approaches to the Detection of M. leprae Infection in Leprosy. In: Talwar, G.P. (eds) Progress in Vaccinology. Progress in Vaccinology, vol 2. Springer, New York, NY. https://doi.org/10.1007/978-1-4612-3508-8_33

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  • DOI: https://doi.org/10.1007/978-1-4612-3508-8_33

  • Publisher Name: Springer, New York, NY

  • Print ISBN: 978-0-387-96734-9

  • Online ISBN: 978-1-4612-3508-8

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