Advertisement

Relationship of Tobacco Mosaic Virus Gene Expression to Movement Within the Plant

  • William O. Dawson

Abstract

Tobacco mosaic virus (TMV) is the type member of the tobamovirus group that is characterized by 18 × 300 nm virions made up of about 2000 units of a single structural protein and one molecule of plus-sense RNA of approximately 6400 nucleotides. TMV has a wide host range and occurs throughout the world causing substantial crop losses. The virus replicates in almost every cell of the plant and accumulates to unusually high titers. Disease generally is caused by abnormal chloroplast development in infected plants resulting in leaves that are deficient in photosynthetic capacity and exhibit typical “mosaic” symptoms of light and dark green leaves.

Keywords

Coat Protein Tobacco Mosaic Virus Coat Protein Gene Wild Type Virus Alfalfa Mosaic Virus 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Beachy, R. N., and Zaitlin, M. (1977). Characterization and in vitro translation of the RNAs from less-than-full-length, virus related, nucleoprotein rods present in tobacco mosaic virus preparations. Virology 81, 160–169.PubMedCrossRefGoogle Scholar
  2. Blum, H., Gross, H. J., and Beier, H. (1989). The expression of the TMV-specific 30-kDa protein is strongly and selectively enhanced by Actinomycin. Virology 169, 51–61.PubMedCrossRefGoogle Scholar
  3. Dawson, W. O., Bubrick, P., and Grantham, G. L. (1988). Modifications of thetobacco mosaic virus coat protein gene affecting replication, movement, and symptomatology. Phytopathology 78, 783–789.CrossRefGoogle Scholar
  4. Dawson, W. O., Lewandowski, M., Hilf, M. E., Bubrick, P., Raffo, A. J., Shaw, J. J., Grantham, G. L., and Desjardins, P. R. (1989). A tobacco mosaic virus-hybrid expresses and loses and added gene. Virology 172, 285–292.PubMedCrossRefGoogle Scholar
  5. Deom, C. M., Oliver, M. J., and Beachy, R. N. (1987). The 30-kilodalton gene product of tobacco mosaic virus potentiates virus movement. Science 237, 389–393.PubMedCrossRefGoogle Scholar
  6. Culver, J. N., and Dawson, W. O. (1989). Tobacco mosaic virus coat protein: an elicitor of the hypersensitive reaction but not required for the delevopment of mosaic symptoms in Nicotiana sylvestris. Virology 173, 755–758.PubMedCrossRefGoogle Scholar
  7. French, R., Janda, M., and Ahlquist, P. (1986). Bacterial gene inserted in an engineered RNA virus: efficient expression in monocotyledenous plant cells. Science 231, 1294–1297.PubMedCrossRefGoogle Scholar
  8. Godefroy-Colburn, T., Gagey, M., Berna, A., and Stussi-Garaud, C. (1986). A nonstructural protein of alfalfa mosaic virus in the walls of infected tobacco cells. J. Gen.Virol. 67, 2233–2239.CrossRefGoogle Scholar
  9. Hunter, T., Hunt, T., Knowland, J., and Zimmern, D. (1976). Messenger RNA for the coat protein of tobacco mosaic virus. Nature (London) 260, 759–764.PubMedCrossRefGoogle Scholar
  10. Ishikawa, M., Meshi, T., Motoyoshi, F., Takamatsu, N., and Okada, Y. (1986). In vitro mutagenesis of the putative replicase genes of tobacco mosaic virus. Nucl. Acids Res. 14, 8291–8305.PubMedCrossRefGoogle Scholar
  11. Lehto, K., Bubrick, P., and Dawson, W. O. (1990 a). Time course of TMV 30K protein accumulation in intact leaves. Virology 174, 290–293.PubMedCrossRefGoogle Scholar
  12. Lehto, K., and Dawson, W. O. (1990 a). Changing the start codon context of the 30K gene of tobacco mosaic virus from “weak” to “strong” does not increase expression. Virology 173, 169–174.CrossRefGoogle Scholar
  13. Lehto, K., and Dawson, W. O. (1990 b). Replication, stability, and gene expression of tobacco mosaic virus mutants with a second 30K ORF. Virology, in press.Google Scholar
  14. Lehto, K., Grantham, G. L., and Dawson, W. O. (1990 b). Insertion of sequences containing the coat protein subgenomic RNA promoter and leader in front of the tobacco mosaic virus 30K ORF delays its expression and causes defective cell-to-cell movement. Virology 174, 145–157.PubMedCrossRefGoogle Scholar
  15. Leonard, D. A., and Zaitlin, M. (1982). A temperature-sensitive strain of tobacco mosaic virus defective in cell-to-cell movement generates an altered viral-coded protein. Virology 117, 416–424.PubMedCrossRefGoogle Scholar
  16. Linstead, P. J., Hills, G. J., Plaskitt, K. A., Wilson, I. G., Harker, C. L. and Maule, A. J. (1988). The subcellular location of the gene 1 product of cauliflower mosaic virus is consistent with a function associated with virus spread. J. Gen. Virol. 69, 1809–1818.CrossRefGoogle Scholar
  17. Meshi, T., Watanabe, Y., Saito, T., Sugimoto, A., Maeda, T., and Okada, Y. (1987). Function of the 30kd protein of tobacco mosaic virus: involvement in cell-to-cell movement and dispensability for replication. EMBO J. 6, 2557–2563.PubMedGoogle Scholar
  18. Moser, O., Gagey, M.-J., Godfroy-Colburn, T., Ellwart-Tschurtz, M., Nitschko, H., and Mundry, K.-W. (1988). The fate of the transport protein of tobacco mosaic virus in systemic and hypersensitive tobacco hosts. J. Gen. Virol. 69, 1367–1373.CrossRefGoogle Scholar
  19. Nishiguchi, M., Motoyoshi, F., and Oshima, N. (1978). Behaviour of a temperature sensitive strain of tobacco mosaic virus: Its behaviour in tomato leaf epidermis. J. Gen. Virol. 39, 53–61.CrossRefGoogle Scholar
  20. Pelham, H. R. B. (1978). Leaky UAG termination codon in tobacco mosaic virus. Nature (London) 272, 469–471.PubMedCrossRefGoogle Scholar
  21. Rushing, A. E., Sunter, G., Gardiner, W. E., Dute, R. R., and Bisaro, D. M. (1987). Ultrastructural aspects of tomato golden mosaic virus infection in tobacco. Phytopatology 77, 1231–1236.CrossRefGoogle Scholar
  22. Siegel, A., Montgomery, V. H. I., and Kolacz, K. (1976). A messenger RNA for coat protein isolated from tobacco mosaic virus infected tissue. Virology 73, 363–371.PubMedCrossRefGoogle Scholar
  23. Siegel, A., Zaitlin, M., and Sehgal, O. P. (1962). The isolation of defective tobacco mosaic virus strains. Proc. Natl. Acad. Sci. USA 48, 1845–1851.PubMedCrossRefGoogle Scholar
  24. Stussi-Garaud, C., Garaud, J., Berna, A., and Godefroy-Colburn, T. (1987). In situ localization of an alfalfa mosaic virus non-structural protein in plant cell walls: correlation with virus transport. J. gen. Virol. 68, 1779–1784.CrossRefGoogle Scholar
  25. Watanabe, Y., Emori, Y., Ooshika, L, Meshi, T., Ohno, T., and Okada, Y. (1984). Synthesis of TMV specific RNAs and proteins at the early stage of infection in tobacco protoplasts: transient expression of the 30K protein and its mRNA. Virology 133, 18–24.PubMedCrossRefGoogle Scholar
  26. Watanabe, Y., Ooshika, I., Meshi, T., and Okada, Y. (1986). Subcellular localization of the 30K protein in TMV-inoculated tobacco protoplasts. Virology 152, 414–420.PubMedCrossRefGoogle Scholar
  27. Wellink, J., and van Kammen, A. (1989). Cell-to-cell transport of cowpea mosaic virus requires both 58K/48K proteins and the capsid proteins. J. Gen. Virol. 70, 2279–2286.CrossRefGoogle Scholar

Copyright information

© Springer-Verlag New York Inc. 1990

Authors and Affiliations

  • William O. Dawson
    • 1
  1. 1.Department of Plant Pathology and Genetics Graduate GroupUniversity of CaliforniaRiversideUSA

Personalised recommendations