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Purification of A1 Adenosine Receptor of Rat Brain Membranes by Affinity Chromatography

  • H. Nakata
Conference paper

Abstract

Adenosine receptors are one of the receptors which are coupled with adenylate cyclase system via G proteins. A1 adenosine receptor (ADOR) which is coupled to adenylate cyclase in the inhibitory manner has been well characterized pharmacologically and biochemically in various tissues. However, more detailed molecular characterization of ADOR has been difficult mainly due to the lack of a purified receptor preparation. Recently, an affinity chromatography system was developed for the purification of ADOR from rat brain membranes using xanthine amine congener (XAC) as an immobilized ligand (Nakata, 1989a), although only 150-fold purification of ADOR was achieved in that study. In this report, an improvement of the affinity chromatography and the purification of ADOR from rat brain membranes to apparent homogeneity are presented (Nakata, 1989b).

Keywords

Adenosine Receptor Apparent Homogeneity Adenylate Cyclase System Immobilize Ligand Typical ADOR 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Nakata, H., 1989a, Affinity chromatography of A1 adenosine receptors of rat brain membranes, Mol. Pharmacol. 35:780–786.Google Scholar
  2. Nakata, H., 1989b, Purification of A1 adenosine receptor from rat brain membranes, J. Biol. Chem. in press.Google Scholar
  3. Stiles, G.L., and Jacobson, K.A., 1988, High affinity acylating antagonists for the A1 adenosine receptor: identification of binding subunit, Mol. Pharmacol. 34:724–728.PubMedGoogle Scholar

Copyright information

© Springer-Verlag New York Inc. 1990

Authors and Affiliations

  • H. Nakata

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