Mechanism for Dual Regulation of the Particulate cGMP-Inhibited cAMP Phosphodiesterase in Rat Adipocytes by Isoproterenol and Insulin

  • C. J. Smith
  • E. Degerman
  • V. Vasta
  • H. Tornqvist
  • P. Belfrage
  • V. C. Manganiello
Conference paper


Lipolysis is dually regulated in the rat adipocyte: it is promoted by agents which increase cellular cAMP and antagonized by agents which decrease the synthesis and/or increase degradation of cAMP. The receptor-dependent activations and inhibitions of adenylate cyclase are mediated by the respective GTP-binding transductional proteins, Gs and Gi (Gilman, 1987). Thus, catecholamines, ACTH, and glucagon activate adenylate cyclase and effect the breakdown of triglyceride to glycerol and free fatty acids, via cAMP-dependent protein kinase- (A-kinase) mediated phosphorylation of a hormone-sensitive lipase (Fain, 1980; figure 1). Conversely, basal adenylate cyclase in the rat adipocyte appears to be under tonic inhibition by adenosine, an antilipolytic agent, since incubation of cells with adenosine deaminase (ADA; Schwabe et al., 1975; Honnor et al., 1985) or with pertussis toxin (which inactivates Gi; Gilman, 1987) promotes increases in cellular cAMP, A-kinase and/or lipolysis (Olansky ei al., 1983; Smith ei al., 1989).


Adenylate Cyclase Pertussis Toxin Cellular cAMP cAMP Phosphodiesterase Antilipolytic Action 
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Copyright information

© Springer-Verlag New York Inc. 1990

Authors and Affiliations

  • C. J. Smith
  • E. Degerman
  • V. Vasta
  • H. Tornqvist
  • P. Belfrage
  • V. C. Manganiello

There are no affiliations available

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