Effects of Chemoexcitatory Agents on K+ Currents of Isolated Type I Cells from Neonatal Rat Carotid Body

  • C. Peers
  • J. O’Donnell


The ability of the carotid bodies to detect changes in arterial oxygen, carbon dioxide, and pH levels is well documented, and there is much evidence to support the idea that the catecholamine-containing type I glomus cells of the carotid body are the primary chemosensory element (1). However, the cellular mechanisms by which the carotid body transduces a change in arterial gas or pH levels into altered carotid sinus nerve activity are almost completely unknown. As ion channels play fundamental roles in stimulus-response coupling in all sensory cells studied thus far, it is of obvious importance to investigate the classes of ion channels present in type I cells and their possible roles in chemotransduction. Here we report electrophysiological studies of ionic currents recorded in isolated neonatal rat type I cells using the whole-cell patch-clamp technique (2).


Current Amplitude Carotid Body Potassium Current Bath Application Bathing Medium 
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  1. 1.
    Fidone SJ, Gonzalez C (1986). Initiation and control of chemoreceptor activity in the carotid body. In: Fishman AP, Cherniack NS, Widdicome JG, Geiger SR (Eds). Handbook of Physiology III: The Respiratory System, Vol. 2. part 1, Bethesda MD: American Physiological Society, pp 247–312.Google Scholar
  2. 2.
    Hamill OP, Marty A, Neher E, Sakmann B, Sigworth FJ (1981). Improved patch clamp techniques for high-resolution current recording from cells and cell-free membrane patches. Pflügers Archiv 391: 85–100.PubMedCrossRefGoogle Scholar
  3. 3.
    Anichkov SV, Belen’kii ML (1963). Pharmacology of the Carotid Body Chemoreceptors, Oxford: Pergamon Press, pp 176–186.Google Scholar
  4. 4.
    Ashcroft FM (1988). Adenosine 5’-triphosphate-sensitive potassium channels. Ann Rev Neurosci 11: 97–118.PubMedCrossRefGoogle Scholar
  5. 5.
    Nye PCG, Maxwell DL, Quirk P, Cook C (1989). The carotid body and almitrine bismesylate. In: Eyzaguirre et al. (Eds). Arterial Chemoreception, New York: Springer-Verlag, pp. 199–206 (this volume).Google Scholar
  6. 6.
    Biscoe TJ, Purves MJ, Sampson SR (1970). The frequency of nerve impulses in single carotid body chemoreceptor afferent fibres recorded in vivo with intact circulaton. J Physiol (Lond) 208: 121–131.Google Scholar
  7. 7.
    Iwatsuki N, Petersen OH (1985). Inhibition of Ca2+-activated K+ channels in pig pancreatic acinar cells by Ba2+, Ca2+, quinine and quinidine Biochim Biophys Acta 819: 249–257.CrossRefGoogle Scholar
  8. 8.
    Armstrong CM, Swenson RP, Taylor SR (1982). Block of squid axon K channels by internally and externally applied barium ions. J Gen Physiol 80: 663–682.PubMedCrossRefGoogle Scholar
  9. 9.
    O’Donnell J, Ashcroft FM, Brown HF, Nye PCG. In: Lahiri S, Forster RE, Davies RO, Pack AI (Eds). Chemoreceptors and Reflexes in Breathing. New York: Oxford University Press, 1989, pp 133–138.Google Scholar
  10. 10.
    Ashcroft FM, O’Donnell J (1987). Tolbutamide, an inhibitor of ATP-sensitive K channels, excites carotid body chemoreceptors in the cat. J Physiol (Lond) 394: 66 P.Google Scholar
  11. 11.
    Delpiano MA, Heschler J, Acker H, Pietruschka F (1988). Ionic channels in rabbit carotid body Type-I cells. Pflügers Archiv suppl 1. 411: 134.Google Scholar
  12. 12.
    López-Barneo J, López-López J, Urena J, Gonzalez C (1988). Chemotransduction in the carotid body: K+ current modulated by Po2 in type I chemoreceptor cells. Science 241: 580–582.PubMedCrossRefGoogle Scholar
  13. 13.
    Duchen MR, Caddy KWT, Kirby GC, Patterson DL, Ponte J, Biscoe TJ (1988). Biophysical studies of the cellular elements of the rabbit carotid body. Neurosci 26: 291–311.CrossRefGoogle Scholar

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© Springer-Verlag New York Inc. 1990

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  • C. Peers
  • J. O’Donnell

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