Electrical Activity of Mouse Carotid Body as Monitored by the Fluorescent Probe diS-C3-5: Effects of Different Agents

  • A. Gual
  • C. Eyzaguirre
Conference paper


Voltage-sensitive dyes have allowed measurement of the potential difference across cell or organelles membranes, membrane vesicles, and cell complexes (1). This technique is based on the observation that changes in potential across membranes stained with certain dyes are accompanied by changes in the optical properties of the preparation; the data obtained by this method coincide with those obtained from the same cellular components with conventional methods (2). Some of these dyes respond to membrane potential changes in microseconds, whereas others, such as those used in this study, respond in seconds allowing the monitoring of slow electrical signals (1).


Carotid Body Fluorescence Change Fluorescence Increase Glomus Cell Membrane Potential Change 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Waggoner AS (1979). Dye indicators of membrane potential. Ann Rev Biophys Bioeng 8: 47–68.CrossRefGoogle Scholar
  2. 2.
    Cohen LB, Salzberg BM, Davila HV, Ross WN, Landowne D, Waggoner AS, Wang CH (1974). Changes in axon fluorescence during activity: molecular probes of membrane potential. J Membrane Biol 36: 1–36.CrossRefGoogle Scholar
  3. 3.
    Gual A (1984). Use of voltage-sensitive dyes in carotid body studies. In: DJ Pallot (Ed). The Peripheral Arterial Chemoreceptors. London, Croom Helm, pp 35–45.Google Scholar
  4. 4.
    Cabrini G, Verkman AS (1986). Potential-sensitive response mechanism of DiS-C3 5 in biological membranes. J Membrane Biol 92: 171–182.CrossRefGoogle Scholar
  5. 5.
    Clarke JA, de Burgh Daly M (1981). A comparative study of the distribution of carotid body type-I cells and periadventitial type-I cells in the carotid bifurcation regions of the rabbit, rat, guinea-pig, and mouse. Cell Tissue Res 220: 753–772.Google Scholar
  6. 6.
    Acker H, Eyzaguirre C, Goldman WF (1985). Redox changes in the mouse carotid body during hypoxia. Brain Res 330: 158–163.PubMedCrossRefGoogle Scholar
  7. 7.
    Acker H, Eyzaguirre C (1987). Light absorbance changes in the mouse carotid body during hypoxia and cyanide poisoning. Brain Res 409: 380–385.PubMedCrossRefGoogle Scholar
  8. 8.
    Baron M, Eyzaguirre C (1977). Effects of temperature on some membrane characteristics of carotid body cells. Am J Physiol 233: C35–C46.PubMedGoogle Scholar
  9. 9.
    Gallego R, Eyzaguirre C, Monti-Bloch L (1979). Thermal and osmotic responses of arterial receptors. J Neurophysiol 42: 665–680.PubMedGoogle Scholar
  10. 10.
    Sato M, Yoshizaki K, Koyano H. (1989). Veratridine stimulation of sodium influx in carotid body cells from newborn rabbits in primary culture. Brain Res 504: 132–135.PubMedCrossRefGoogle Scholar
  11. 11.
    Graves C, Sachs G (1982). Quantitation of corneal endothelial potentials using a carbocyanine dye. Biochim Biophys Acta 685: 27–31.PubMedCrossRefGoogle Scholar
  12. 12.
    Oyama Y, Walker JL, Eyzaguirre C (1986). Intracellular potassium activity, potassium equilibrium potential and membrane potential of carotid body glomus cells. Brain Res 381: 405–408.PubMedCrossRefGoogle Scholar
  13. 13.
    Almaraz L, Gonzalez C, Obeso A (1986). Effects of high potassium on the release of [3H1-dopamine from the cat carotid body in vitro. J Physiol (Lond) 379: 293–307.Google Scholar
  14. 14.
    Eyzaguirre C, Zapata P (1968). Pharmacology of pH effects on carotid body chemoreceptors in vitro. J Physiol (Lond) 195: 589–607.Google Scholar
  15. 15.
    López-Barneo J, Lopez-López JR, Urena J, Gonzalez C (1988). Chemotransduction in the carotid body: K+ current modulated by Po, in type I chemoreceptor cells. Science 241: 580–582.Google Scholar
  16. 16.
    Hayashida Y, Eyzaguirre C (1979). Voltage noise of carotid body type I cells. Brain Res 167: 189–194.PubMedCrossRefGoogle Scholar
  17. 17.
    Eyzaguirre C, Monti-Bloch L, Woodbury JW (1990). Effects of putative neurotransmitters of the carotid body on its own glomus cells. Eur J. Neurosci 2: 77–88.PubMedCrossRefGoogle Scholar
  18. 18.
    Dinger B, Gonzalez C, Yoshizaki K, Fidone S (1985). Localization and function of cat carotid body nicotinic receptors. Brain Res 339: 295–304.PubMedCrossRefGoogle Scholar
  19. 19.
    Goldman WF, Eyzaguirre C (1984). The effect of dopamine on glomus cell membranes in the rabbit. Brain Res 321: 337–340.PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag New York Inc. 1990

Authors and Affiliations

  • A. Gual
  • C. Eyzaguirre

There are no affiliations available

Personalised recommendations