Immunocytochemical Analysis of the Cellular Composition of Atherosclerotic Lesions in the Human Aorta
This report presents the results of immunocytochemical analyses of the cellular composition of atherosclerotic lesions in the human aorta. Aortae obtained at autopsy were immunostained with monoclonal antibodies against human alveolar macrophages (HAM-56), muscle actin (HHF35), the T-200 antigen of lymphocytes and monocytes, and the factor VIII related antigen of endothelial cells. Based on the immunostaining characteristics, three broad categories of lesions were observed. These include: the fibro-fatty lesion, the fibrous lesion, and the advanced lesion. The advantages of using monoclonal antibody techniques over traditional morphologic and histochemical approaches are also discussed.
KeywordsSmooth Muscle Cell Atherosclerotic Lesion Foam Cell Cellular Composition Immunocytochemical Analysis
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- 5.Geer JC, Haust MD (1972) Smooth Muscle Cells in Atherosclerosis. Monographs on Atherosclerosis, Volume 2. Pollak OJ, Simms HS, Kirk SE (eds) Basel-New York: S Karger.Google Scholar
- 6.Huff HF, Heideman CL, Ganbotz JW, Schff DW, Titus JL, Gotto Jr AM (1978) Correlation of apolipoprotein B retention with the structure of atherosclerotic plaques from human aortas. Lab Invest 38:560–567.Google Scholar