Immunocytochemical Analysis of the Cellular Composition of Atherosclerotic Lesions in the Human Aorta

  • Toyohiro Tsukada
  • Michael E. Rosenfeld
  • Allen M. Gown
  • Russell Ross
Conference paper


This report presents the results of immunocytochemical analyses of the cellular composition of atherosclerotic lesions in the human aorta. Aortae obtained at autopsy were immunostained with monoclonal antibodies against human alveolar macrophages (HAM-56), muscle actin (HHF35), the T-200 antigen of lymphocytes and monocytes, and the factor VIII related antigen of endothelial cells. Based on the immunostaining characteristics, three broad categories of lesions were observed. These include: the fibro-fatty lesion, the fibrous lesion, and the advanced lesion. The advantages of using monoclonal antibody techniques over traditional morphologic and histochemical approaches are also discussed.


Smooth Muscle Cell Atherosclerotic Lesion Foam Cell Cellular Composition Immunocytochemical Analysis 
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Copyright information

© Springer-Verlag New York Inc. 1990

Authors and Affiliations

  • Toyohiro Tsukada
  • Michael E. Rosenfeld
  • Allen M. Gown
  • Russell Ross

There are no affiliations available

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