Mechanism Regulating Recruitment of CD11b/CD18 to the Cell Surface is Distinct From That Which Induces Adhesion in Homotypic Neutrophil Aggregation
In response to activation by certain stimuli, neutrophils undergo changes in surface properties that result in their formation of specific cellular aggregates (1). This phenomenon is being intensively studied as a model of induced homotypic cell-to-cell interaction. Evidence that heterodimeric glycoproteins of the Leu-CAM family are involved in mediating neutrophil aggregation rests on several findings; (i) most monoclonal antibodies (MAb) to CD18, the common 95 kD beta subunit (β2) abolish neutrophil aggregation (2); (ii) several of the MAb to CD11b, the 165 kD alpha subunit (also referred to as αM, CR3, or MAC-1 inhibit this response (2,3); and (iii) neutrophils from individuals genetically deficient in these molecules cannot be stimulated to aggregate (4).
KeywordsSuperoxide Integrin HEPES Dition Fluorescein
Unable to display preview. Download preview PDF.
- 3.Buyon JP, Hopkins P, Abramson SB, Winchester R: Differential participation of epitopes on alpha and beta chains of the LFA family in neutrophil aggregation as defined by workshop monoclonal antibodies (MoAbs), in McMichael AJ et al. (eds); Leukocyte Typing HI. White Cell Differentiation Antigens. Oxford, Oxford University Press, pp 844–848, 1987.Google Scholar
- 4.Anderson DC, Schmalstieg FC, Finegold MJ, Hughes BJ, Rothlein R, Miller LJ, Kohl S, Tosi MF, Jacobs RL, Waldrop TC, Goldman AS, Shearer WT, Springer TA: The severe and moderate phenotypes of heritable Mac-1, LFA-1 deficiency: Their quantitive definition and relation to leukocyte dysfunction and clinical features. J Infect Dis 152:668, 1985.PubMedCrossRefGoogle Scholar
- 6.Jones D: Characterization of a new mobilizable Mac-1 (CD11/CD18) pool that co-localizes with gelatinase in human neutrophils, in Springer TA et al. (eds.), Leukocyte Adhesion Molecules. New York, Springer Verlag, ch 8, 1989.Google Scholar
- 8.Arnaout MA, Spits H, Terhorst C, Pitt J, Todd, RF III: Deficiency of a leukocyte surface glycoprotein (LFA-1) in two patients with Mol deficiency: Effects of cell activation on Mo1/LFA-1 surface expression in normal and deficient leukocytes. J Clin Invest 74:1291–1300, 1984.PubMedCrossRefGoogle Scholar
- 10.O’Shea JJ, Brown EJ, Seligmann BE, Metcalf JA, Frank MM, Gallin JI: Evidence for distinct intracellular pools of receptors for C3b and C3bi in human neutrophil. J Immunol 134:2580–2587, 1984.Google Scholar
- 13.Bernstein ID, Self S: Joint report of the myeloid section of the Second International Workshop on Human Leukocyte Differentiation Antigens, in Rein-herz EL, Haynes BF, Nadler LM, Bernstein ID (eds): Leukocyte Typing II Vol. 3. New York, Springer-Verlag pp 1–25, 1986.Google Scholar
- 20.Buyon JP, Abramson SB, Phillips M, Slade SG, Weissmann G, Winchester RJ: Phosphorylation of the alpha and beta chains of the CD11/CD18 integrin family: constitutive and activated states. Clin Res 37:425A, 1989.Google Scholar