Specificity and Regulation of CD18-Dependent Adhesions
The ability of polymorphonuclear leukocytes (PMN) to adhere to other cells and substrates is under strict physiological regulation. This is best illustrated by considering PMN circulating within the vasculature. These cells show negligible affinity for the endothelial cells (EC) which surround them. When PMN encounter chemotactic factors this situation is rapidly altered, and the adhesion of PMN to EC is dramatically enhanced. In normal circumstances, stimulated PMN soon break their adhesion to EC and either return to the circulation or leave the vasculature by diapedesis. Thus, PMN possess the means of transiently making then breaking adhesion to EC and other substrates in response to specific stimuli.
KeywordsPhorbol Myristate Acetate Phorbol Myristate Acetate Vitronectin Receptor Fibronectin Receptor Neural Crest Cell Migration
Unable to display preview. Download preview PDF.
- 8.Tuomanen EI, Saukkonen K, Sande S, Cioffe C, Wright SD: Reduction of inflammation, tissue damage, and mortality in bacterial meningitis in rabbits treated with monoclonal antibodies against adhesion-promoting receptors of leukocytes. J Exp Med (in press), 1989.Google Scholar
- 9.Todd III RF, Freyer DR: The CD11/CD18 leukocyte glycoprotein deficiency. Hem/Onc Clin N Am 2:13–31, 1988.Google Scholar
- 14.Law SKA, Gagnon J, Hildreth JE, Wells CE, Willis AC, Wong AJ: The primary structure of the beta-subunit of the cell surface adhesion glycoproteins LFA-1, CR3 and p150,95 and its relationship to the fibronectin receptor. EMBO 7 6:915,1987.Google Scholar
- 28.Ross GD, Thompson RA, Walport MJ, Springer TA, Watson JV, Ward RHR, Lida J, Newman SL, Harrison RA, Lachmann PJ: Characterization of patients with an increased susceptibility to bacterial infections and a genetic deficiency of leukocyte membrane complement receptor type 3 and the related membrane antigen LFA-1. Blood 66:882–890, 1985.PubMedGoogle Scholar
- 31.Wright SD, Jong MTC: Adhesion-promoting receptors on human macrophages recognize E. coli by binding to lipopolysaccharide. J Exp Med 164:1867–1888, 1986.Google Scholar
- 32.Morrison DC, Ulevitch JR: The effects of bacterial endotoxins on host mediating systems. Am J Pathol 93:527–617, 1978.Google Scholar
- 35.Suzuki S, Oldberg A, Hayman EG, Pierschbacher MD, Ruoslahti E: Complete amino acid sequence of human vitronectin deduced from cDNA. Similarity of cell attachment sites in vitronectin and fibronectin. EMBO 74:2519–2524, 1985.Google Scholar
- 37.Lo SK, Wright SD: CR3 mediates binding of PMN to endothelial cells (EC) via its RGD binding, not the LPS binding site. FASEB J 2:A991, 1988.Google Scholar
- 38.Marlin SD, Springer TA. 1987. Purified intercellular adhesion molecule-1 (ICAM-1) is a ligand for lymphocyte function-associated antigen (LFA-1). Cell 51:813–819, 1987.Google Scholar
- 40.Sanderson CJ: 1976. The mechanism of T cell mediated cytotoxicity. II. Morphological studies of cell death by time-lapse microcinematography. Proc R Soc Lond Ser B 92:241–255, 1976.Google Scholar
- 41.Boucaut JC, Darribere T, Poole TJ, Aoyama H, Yamada KM, Thiery JP: Biologically active synthetic peptides as probes of embryonic development: A competitive peptide inhibitor of fibronectin function inhibits gastrulation in amphibian embryos and neural crest cell migration in avian embryos. J Cell Biol 99:1822–1830, 1984.PubMedCrossRefGoogle Scholar
- 57.Hermanowski-Vosatka A, Detmers PA, Goetze O, Silverstein SC, Wright SD: Clustering of binding sites on the plasma membrane enhances cell-cell adhesion. J Biol Chem 263:17,822–17,827, 1988.Google Scholar