• David Linstead


The history of our understanding of human urogenital trichomoniasis and the organism Trichomonas vaginalis, which is the causative agent of this disease, is intimately bound up with the history of efforts to cultivate the organism in vitro. Only with axenic cultivation did it become possible to show conclusively that T. vaginalis was the sole originator of the rather diverse signs and symptoms seen in human infections. The disease has been produced in parasite-free female subjects by inoculation of axenic cultures on a number of occasions, producing some of the pathologic changes characteristic of the naturally acquired infection. Early experiments of this type are described in TrusselPs classic book,1 while later experiments are noted by Honigberg.2


Axenic Culture Urea Plasma Urealyticum Serum Substitute Chick Embryo Extract Dibasic Potassium Phosphate 
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© Springer-Verlag New York Inc. 1990

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  • David Linstead

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