Flow Cytometric Analysis of Class I and II MHC Antigens on Ovine Luteal Cell Types

  • Nicholas Kenny
  • James R. Herman
  • B. George Barisas
  • Deborah A. Roess
Part of the Serono Symposia USA book series (SERONOSYMP)


Secretion of progesterone by the corpus luteum (CL) is essential for the maintenance of early pregnancy in many species (1). The identification and characterization of two functionally and morphologically distinct types of steroidogenic luteal cells has provided several insights into the control of CL function (2). However, cells of the immune system may also play significant roles in the function and control of this ephemeral endocrine gland. Macrophages, lymphocytes, mast cells, monocytes, and eosinophils are found in CL of a variety of species (3). Their presence may indicate some role for the immune system in CL function.


Major Histocompatibility Complex Major Histocompatibility Complex Class Corpus Luteum Estrous Cycle Luteal Cell 
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  1. 1.
    Auletta FJ, Flint APF. Mechanisms controlling corpus luteum function in sheep, cows, nonhuman primates, and women especially in relation to the time of luteolysis. Endocr Rev 1988; 9: 88 – 105.PubMedCrossRefGoogle Scholar
  2. 2.
    Niswender GD, Schwall RH, Fitz TA, Farin CE, Sawyer HR. Regulation of luteal function in domestic ruminants: New concepts. Recent Prog Horm Res 1985; 41: 101 – 51.PubMedGoogle Scholar
  3. 3.
    Adashi EY. Editorial: Cytokine-mediated regulation of ovarian function: En-counters of a third kind. Endocrinology 1989; 124: 2043 – 4.PubMedCrossRefGoogle Scholar
  4. 4.
    Guillet J-G, Lai M-Z, Briner TJ, Buus S, Sette A, Grey HM, Smith JA, Gefter ML. Immunological self, nonself discrimination. Science 1987; 235: 865 – 70.PubMedCrossRefGoogle Scholar
  5. 5.
    Edidin M. Function by association? MHC antigens and membrane receptor complexes. Immunol Today 1988; 9: 218 – 9.PubMedCrossRefGoogle Scholar
  6. 6.
    Daar AS, Fuggle SV, Fabre JW, Ting A, Morris PJ. The detailed distribution of MHC class II antigens in normal human organs. Transplantation 1984; 38: 293 – 8.PubMedCrossRefGoogle Scholar
  7. 7.
    Zauderer M. Origin and significance of autoreactive T cells. Adv Immunol 1989; 45: 417 – 37.PubMedCrossRefGoogle Scholar
  8. 8.
    Bottazzo GF, Pujol-Borrel R, Hanafusa T, Feldman R. Role of aberrant HLADR expression and antigen presentation in induction of endocrine autoimmunity. Lancet 1983;ii:1115–8.Google Scholar
  9. 9.
    Lobel BL, Levy E. Enzymatic correlates of development, secretory function and regression of follicles and corpora lutea in the bovine ovary, EL Formation, development, and involution of corpora lutea. Acta Endocrinol 1968; 59 (suppl 132): 35 – 51.Google Scholar
  10. 10.
    Hume DA, Halpan D, Charlton H, Gordon S. The mononuclear phagocyte system of the mouse defined by immunohistochemical localization of antigen F4/ 80: Macrophages of endocrine organs. Proc Natl Acad Sci USA 1984; 81: 4174 – 7.CrossRefGoogle Scholar
  11. 11.
    Adams EC, Hertig AT. Studies on the human corpus luteum, I. Observations on the ultrastructure of development and regression of the luteal cells during the menstrual cycle. J Cell Biol 1969; 41: 696 – 715.PubMedCrossRefGoogle Scholar
  12. 12.
    Paavola LG. The corpus luteum of the guinea pig, IV. Fine structure of macrophages during pregnancy and postpartum luteolysis, and the phagocytosis of luteal cells. Am J Anat 1979; 154: 337 – 64.CrossRefGoogle Scholar
  13. 13.
    Bagavandoss P, Wiggins RC, Kunkel L, Remick DG, Keyes PL. Tumor necrosis factor production and accumulation of inflammatory cells in the corpus luteum of pseudopregnancy and pregnancy in rabbits. Biol Reprod 1990; 42: 367 – 76.PubMedCrossRefGoogle Scholar
  14. 14.
    Nett TM, McClellan MC, Niswender GD. Effects of prostaglandins on the ovine corpus luteum: Blood flow, secretion of progesterone and morphology. Biol Reprod 1976; 15: 66 – 78.PubMedCrossRefGoogle Scholar
  15. 15.
    Fairchild DL, Pate JL. Interferon-y induction of major histocompatibility complex antigens on cultured bovine luteal cells. Biol Reprod 1989; 40: 453 – 7.PubMedCrossRefGoogle Scholar
  16. 16.
    Tabibzadeh SS, Bettica A, Gerber MA. Variable expression of la antigens in human endometrium and in chronic endometritis. Am J Clin Pathol 1986; 86: 153 – 60.PubMedGoogle Scholar
  17. 17.
    Tabibzadeh SS, Gerber MA, Satyaswaroop PG. Induction of HLA-DR antigen expression in human endometrial epithelial cells in vitro by recombinant γ-interferon. Am J Pathol 1986; 125: 90 – 6.PubMedGoogle Scholar
  18. 18.
    Charriere J. Immune mechanisms in autoimmune thyroiditis. Adv Immunol 1989; 46: 263 – 334.CrossRefGoogle Scholar
  19. 19.
    Londei M, Lamb JR, Bottazzo GF, Feldmann M. Epithelial cells expressing aberrant MHC class II determinants can present antigen to cloned human T- cells. Nature 1984; 312: 639 – 41.PubMedCrossRefGoogle Scholar
  20. 20.
    Fairchild DL, Pate JL. MHC antigen expression on bovine luteal cells varies during the estrous cycle. Biol Reprod 1989;40 (suppl l):236a.Google Scholar
  21. 21.
    Harrison LM, Kenny N, Niswender GD. Progesterone production, LH receptors, and oxytocin secretion by ovine luteal cell types on days 6, 10, and 15 of the estrous cycle and day 25 of pregnancy. J Reprod Fertil 1987; 79: 539 – 48.PubMedCrossRefGoogle Scholar
  22. 22.
    Gogolin-Ewens KJ, Mackay CR, Mercer WR, Brandon MR. Sheep lymphocyte antigens (OLA), I. Major histocompatibility complex class I molecules. Immu-nology 1985; 56: 717 – 22.Google Scholar
  23. 23.
    Puri N, Mackay CR, Brandon MR. Sheep lymphocyte antigens (OLA), n. MHC class two molecules. Immunology 1985; 56: 725 – 33.PubMedGoogle Scholar
  24. 24.
    Herman JR, Boullier JA, Roess DA, Parr RO, Barisas BG. Examination of cytokinetics and ploidy obtained from paraffin-embedded prostatic tissue. Cytometry, 1990.Google Scholar

Copyright information

© Springer-Verlag New York, Inc. 1991

Authors and Affiliations

  • Nicholas Kenny
  • James R. Herman
  • B. George Barisas
  • Deborah A. Roess

There are no affiliations available

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