Fetal Calf Serum: Eliciting Phosphorylation of Ca++/Calmodulin-Dependent Protein Kinase II in Cultured Rat Granulosa Cells

  • Takashi Ohba
  • Yasutaka Ohta
  • Kohji Miyazaki
  • Hitoshi Okamura
  • Eishichi Miyamoto
Part of the Serono Symposia USA book series (SERONOSYMP)


It is well known that a number of factors affect the proliferation, differentiation, and steroidogenesis of cultured rat granulosa cells. Two multifunctional protein kinases, such as cAMP-dependent protein kinase and protein kinase C, are thought to mediate intracellular signals in granulosa cells. Follicle stimulating hormone (FSH) induces granulosa cell differentiation and the steroidogenesis, which are mediated by the action of cAMP-dependent protein kinase (1–3). In contrast, phorbol ester inhibits FSH actions, such as cAMP formation, LH receptor expression, cholesterol side-chain cleavage enzyme synthesis, and progesterone production (4–7) via the activation of protein kinase C.


Granulosa Cell Luteal Cell Progesterone Production Culture Granulosa Cell Dibutyryl Adenosine 
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Copyright information

© Springer-Verlag New York, Inc. 1991

Authors and Affiliations

  • Takashi Ohba
  • Yasutaka Ohta
  • Kohji Miyazaki
  • Hitoshi Okamura
  • Eishichi Miyamoto

There are no affiliations available

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